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Tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as target

A protein vaccine and vaccine technology, applied in the field of tumor DNA vaccine and virus vector vaccine, can solve the problems of FAP gene silencing

Active Publication Date: 2016-08-24
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(4) Modification of FAP gene leads to FAP gene silencing

Method used

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  • Tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as target
  • Tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as target
  • Tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as target

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0020] 1. The preparation method and conditions of the above-mentioned fragment FAPα

[0021] 1. Preparation of FAPα

[0022] FAPα contains 2281 bases, and its GenBank number is NM_004460 (SEQ ID NO: 1). A pair of primers were designed according to its sequence, and the gene fragment of FAPα was amplified in the cDNA of the human intestinal cancer patient sample, and the sequence was the base sequence shown in SEQ NO:1. The specific method is as follows:

[0023] 1) Construction, identification and sequence analysis of pGEM-T-FAP

[0024] The PCR product of the amplified FAPα from the cDNA of the intestinal cancer patient sample was ligated with the pGEM-T-easy vector to obtain the pGEM-T-FAP plasmid containing FAPα. Confirmed to be correct by sequence analysis.

[0025] 2. The method of obtaining the recombinant vaccine and the corresponding conditions

[0026] 1. DNA vector vaccine

[0027] 1) Construction of CpVR-FAP

[0028] CpVR vector contains CpG motif, CMV promo...

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Abstract

The invention relates to the field of tumor DNA vaccines and virus vector vaccines with fibroblast activation protein alpha as a target, in particular to recombinant DNA carrier CpVR-FAP, recombinant adenovirus Ad-FAP and recombinant poxvirus MVA-FAP vaccines, DNA vaccines, virus vector vaccines and optimal combination of immune ways of the virus vector vaccines or application of combination of the vaccines and cyclophosphamide in preparation of anti-tumor vaccines.

Description

technical field [0001] The invention relates to the field of tumor DNA vaccine and virus vector vaccine. Specifically, the present invention relates to recombinant DNA vector CpVR-FAP, recombinant adenovirus Ad-FAP and recombinant poxvirus MVA-FAP vaccine, as well as the use of DNA vaccine and virus vector vaccine combination in preparing anti-tumor vaccine. Background technique [0002] Fibroblast activating protein α (FAPα) is an antigen molecule specifically expressed on the surface of tumor-associated fibroblasts (CAF), which belongs to the serine protease family and has collagenase and dipeptidyl peptidase activities. It plays an important role in the degradation and reconstruction of tumors, and is involved in tumor growth, invasion and metastasis. FAPα is selectively expressed in more than 90% of malignant epithelial tumors (such as breast cancer, ovarian cancer, lung cancer, colon cancer, pancreatic cancer, skin melanoma, etc.) etc.), in embryonic tissues, healing w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/00A61P35/00C12N15/85C12N7/01C12N15/861C12N15/863
CPCA61K39/0011A61K2039/53C12N7/00C12N15/85C12N15/86C12N2710/10021C12N2710/24021C12N2800/107
Inventor 张海红于湘晖孔维夏秋耿飞
Owner JILIN UNIV
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