The invention relates to a genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of the biochemical site marker. The genetic biochemical site marker for the cerebral ischemia inbred line meriones is selected from at least one of glucose-6-phosphate dehydrogenase-1, esterase-3, carbonic anhydrase-2, alkaline phosphatase-1, isocitrate dehydrogenase-1, malic enzyme-1, glucose phosphate translocase-1, renal catalase-2, peptidase-3, glucose phosphate isomerase-1, hemoglobin-beta chain, transferring, esterase-1, esterase-10, glycerol phosphate dehydrogenase-1, beta-glucuronidase-1, esterase-2, lactic dehydrogenase regulator-1, serum protein-1, amylase-1, esterase-6, esterase-8, esterase-9, esterase-4, catalase-1 and esterase-12.