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37 results about "Isocitrate Dehydrogenase (NAD+)" patented technology
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Fused-bicyclic aryl quinolinone derivatives as mutant-isocitrate dehydrogenase inhibitors
The invention relates to inhibitors of mutant isocitrate dehydrogenase (mt-IDH) proteins with neomorphic activity useful in the treatment of cell-proliferation disorders and cancers, having the Formula:where A, B, U, V, Z, W1, W2, W3, and R1-R6 are described herein.
Owner:FORMA THERAPEUTICS INC
Fused-bicyclic aryl quinolinone derivatives as mutant-isocitrate dehydrogenase inhibitors
The invention relates to inhibitors of mutant isocitrate dehydrogenase (mt-IDH) proteins with neomorphic activity useful in the treatment of cell-proliferation disorders and cancers, having the Formula:where A, B, U, V, Z, W1, W2, W3, and R1-R6 are described herein.
Owner:FORMA THERAPEUTICS INC
Pyridin-2(1H)-one quinolinone derivatives as mutant-isocitrate dehydrogenase inhibitors
ActiveUS10005734B2Organic active ingredientsNervous disorderIsocitrate Dehydrogenase (NAD+)Isocitrate Dehydrogenase-I
The invention relates to inhibitors of mutant isocitrate dehydrogenase (mt-IDH) proteins with neomorphic activity useful in the treatment of cell-proliferation disorders and cancers, having the Formula:where A, B, W1, W2, W3, and R1-R8 are described herein.
Owner:FORMA THERAPEUTICS INC
Construction and applications of corynebacterium glutamicum mutant strain for producing L-homoserine
The invention discloses construction and applications of a corynebacterium glutamicum mutant strain for producing L-homoserine, and belongs to the technical field of fermentation engineering. Corynebacterium glutamicum ATCC 13032 is taken as a starting strain to knock out regulatory protein McbR, homoserine kinase, transport protein MetD, phosphoenolpyruvate carboxykinase; the expression of isocitrate dehydrogenase is down regulated; transport protein BrnFE, aspartic semialdehyde dehydrogenase and homoserine dehydrogenase are overexpressed; and the expression of aspartate kinase, pyruvate carboxylase and the aspartate kinase I derived from escherichia coli are enhanced. Shake flask culture can be performed on the mutant strain for 48 h, and the yield of L-homoserine can reach 8.8 g / L.
Owner:JIANGNAN UNIV
Cold-resistant gene engineering application method of rice OsICE2 gene
InactiveCN103290050AReduce expressionIncrease increaseFermentationGenetic engineeringBiotechnologyGenetically modified rice
The invention discloses a cold-resistant gene engineering application method of rice OsICE2 gene. The method includes: (1) gene cloning, (2) vector construction, (3) transgenosis, (4) analysis of the cold-resistant phenotype of OsICE2 by a transgenic technology, and (5) comparative analysis of a transgenic plant and a control plant in the cold-resistant physiological aspect. By the steps, the method determines the influence of OsICE2 gene on protein expression quantity under low temperature stress, and finds that a transgenic rice strain can slow down the reduction of protein expression quantity of RuBi sCO subunit binding-protein alpha subunit, RuBi sCO activase small isoform precursor, Chlorophyll a-b binding protein and isocitrate dehydrogenase under low temperature stress, and improves the expression increase range of 70 kDa heat shock-related protein.
Owner:HUAIYIN TEACHERS COLLEGE
Anti-tumor medicament based on alpha ketoglutaric acid and derivatives thereof
The invention belongs to the technical field of biomedicine and relates to an anti-tumor medicament based on alpha ketoglutaric acid and derivatives thereof. The derivatives comprise various ester derivatives, amide derivatives and ether derivatives; and the alpha ketoglutaric acid comprises chemical analogues and derivatives thereof. The results of in-vitro experiments indicate that the mutation of isocitrate dehydrogenase in gliomas results in the loss of enzyme activities, the alpha ketoglutaric acid capable of growing in cells is obvious reduced, the levels of anaerobic inductor factors capable of inducing tumors and promoting the growth of the tumors are obviously improved, and the phenomenon can be reversed by exogenously replenishing absorbable alpha ketoglutaric acid. Therefore, an absorbable alpha ketoglutaric acid compound can be used for treating the gliomas, pancreatic cancers, breast cancers, liver cancers, lung cancers, gastric cancers, colorectal cancers, colon cancer and melanomas.
Owner:FUDAN UNIV
Corynebacterium glutamicum capable of increasing lysine yield and constructing method of corynebacterium glutamicum
InactiveCN108441525AAchieve balanceBacteriaMicroorganism based processesSphaerotrichia divaricataStreptococcus mutans
The invention discloses corynebacterium glutamicum capable of increasing lysine yield and a constructing method of the corynebacterium glutamicum, and belongs to the technical field of genetic engineering. A genetic engineering method is adopted for substituting IDH (isocitrate dehydrogenase) coding gene icdCg in corynebacterium glutamicum RG for IDH coding gene icdSm in streptococcus mutans, andaccordingly, affinity of IDH to different redox cofactors is regulated, the problem of intracellular redox imbalance in the synthesis process of L-lysine is solved, and the L-lysine accumulation capacity of the strain is improved. Tank (5L fermentation tank) experiments show that L-lysine accumulation amount of the recombinant bacterium reaches 121.4 g / L. Redox cofactor affinity of IDH in corynebacterium glutamicum is successfully changed, problem of intracellular redox imbalance during synthesis of L-lysine is solved, and a new idea for breeding the L-Lysine high-yield strain is provided.
Owner:JIANGNAN UNIV
Double-aryl maleimide compound, pharmaceutically acceptable salt thereof, method for preparing double-aryl maleimide compound and pharmaceutically acceptable salt and application of double-aryl maleimide compound and pharmaceutically acceptable salt
The invention discloses a double-aryl maleimide compound, pharmaceutically acceptable salt thereof, a method for preparing the double-aryl maleimide compound and the pharmaceutically acceptable salt and application of the double-aryl maleimide compound and the pharmaceutically acceptable salt. The double-aryl maleimide compound and the pharmaceutically acceptable salt can be particularly used as isocitrate dehydrogenase 1 (IDH1) mutant inhibitors and can be applied to treating malignant tumor such as glioma and acute myeloid leukemia. The double-aryl maleimide compound, the pharmaceutically acceptable salt, the method and the application have the advantages that the double-aryl maleimide compound has efficiently selective IDH1 mutant inhibitory activity, and accordingly the double-aryl maleimide compound and the pharmaceutically acceptable salt can be used for treating the IDH1 mutant mediated malignant tumor such as the glioma and the acute myeloid leukemia; the double-aryl maleimide compound, the pharmaceutically acceptable salt and the method are reasonable in design, and the method is simple and practical.
Owner:ZHEJIANG UNIV OF TECH +1
Steroidal compounds as well as extraction method and application thereof
ActiveCN108440471AEnhanced inhibitory effectOrganic active ingredientsSteroidsIsocitrate dehydrogenasePhotochemistry
The invention belongs to the technical field of medicines, and discloses an extraction method for steroidal components in glossy ganoderma and an application of the steroidal components. The compoundshave structures represented by a formula (I) and a formula (II) shown in the description. The compounds and compositions of the compounds disclosed by the invention have an inhibitory effect on glycolysis first rate-limiting enzyme HK-2 or isocitrate dehydrogenase (IDH1), thereby having a significant anti-tumor effect; and the compounds have good research and development prospects.
Owner:SHENYANG PHARMA UNIVERSITY
Compounds and methods for the treatment of isocitrate dehydrogenase related diseases
Owner:THE BROAD INST INC +1
Method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi regulation
ActiveCN113136382AGrowth is not affectedBlocking tributary metabolic pathwaysMicroorganism based processesFermentationIsocitrate Dehydrogenase (NAD+)Gene expression level
The invention provides a method for synthesizing glyoxylic acid by utilizing corynebacterium glutamicum based on CRISPRi (clustered regularly interspaced short palindromic repeats i) regulation. According to the method, an acetaldehyde dehydrogenase coding gene of the corynebacterium glutamicum is replaced through a dCas9 expression cassette gene; a lactic dehydrogenase encoding gene of corynebacterium glutamicum is replaced by sgRNA transcription units of an isocitrate dehydrogenase encoding gene and a malic acid synthetase encoding gene, an isocitrate lyase encoding gene of the corynebacterium glutamicum is over-expressed to obtain a corynebacterium glutamicum engineering strain, and the engineering strain is cultured to ferment and synthesize glyoxylic acid. Corynebacterium glutamicum is used as a starting strain, a lactic dehydrogenase coding gene and an acetaldehyde dehydrogenase coding gene which are key enzymes of branch metabolism of the corynebacterium glutamicum are knocked out, a CRISPRi regulation system is established, expression levels of an isocitrate dehydrogenase coding gene and a malic acid synthase coding gene are reduced, and meanwhile, an isocitrate lyase coding gene is overexpressed, so that a method for biosynthesizing glyoxylic acid by using corynebacterium glutamicum is established.
Owner:HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Compounds and methods for the treatment of isocitrate dehydrogenase related diseases
The invention relates to compounds of Formula I or a pharmaceutically acceptable salt, ester or prodrug thereof:
Owner:THE BROAD INST INC +1
Isocitrate dehydrogenase, gene thereof, and use of the same in the treatment of obesity, hyperlipidemia, and fatty liver in lipid biosynthesis
The present invention relates to a cytosolic isocitrate dehydrogenase, its gene, and its use in the treatment of obesity, hyperlipidemia, and fatty liver. The expression of the IDPc gene and the concomitant increase in IDPc level bring about an increase in the cellular level of NADPH, which causes the lipid deposition in adipocytes, leading to obesity and fatty liver. A decrease in the cellular level of NADPH, resulting from the suppression of the gene expression of IDPc, has the effect of inhibiting the lipid deposition in adipocytes. Further, by taking advantage of the suppressive or inhibitory effects of isocitrate dehydrogenase inhibitors, pharmaceutically effective materials for the prophylaxis and treatment of obesity, hyperlipidemia and fatty liver can be developed.
Owner:TG LIFE IND +1
IDH gene dsRNA capable of improving termite control effect of metarhizium anisopliae
ActiveCN111849984AStrong specificityReduced resistance to Metarhizium anisopliae infectionBiocideAnimal repellantsBiotechnologyIsocitrate Dehydrogenase (NAD+)
The invention relates to IDH gene dsRNA capable of improving the termite control effect of metarhizium anisopliae. The sequence design of the dsRNA is derived from a key metabolic enzyme gene, namelyan isocitrate dehydrogenase (IDH) gene, in bodies of the eupolyphaga nigromaculata and the termites nigromaculata. An IDH dsRNA template is synthesized by utilizing a specific primer of the IDH gene,and then dsIDH is generated through transcription of a T7 in-vitro transcription system. After dsIDH is introduced into a termite body by adopting an RNAi technology, the IDH gene expression is reduced, the antifungal activity of the termite is remarkably reduced, the apoptosis rate is increased, the infection level is increased, the IDH activity is reduced, the ATP level is reduced, the movementdistance is reduced, and the death rate of the termite is remarkably increased. The dsIDH can significantly improve the control effect of metarhizium anisopliae on termites, is environmentally friendly and safe to people and livestock, and has good research and application prospects.
Owner:宜昌市金通白蚁防治有限公司 +1
Enzyme activity detection kit for alpha-oxoglutarate dependent enzyme and application thereof
PendingCN109929908AMicrobiological testing/measurementIsocitrate Dehydrogenase (NAD+)Mutated protein
The invention discloses an enzyme activity detection kit for alpha-oxoglutarate dependent enzyme and an application thereof. The kit includes one of reduced coenzyme II (NADPH) or reduced coenzyme I (NADH), an isocitrate dehydrogenase IDH mutant protein and buffer salt, and the IDH mutant protein refers to an IDH mutant protein having a novel function of catalyzing alpha-oxoglutarate (2-OG) obtained after mutation of a specific amino acid of IDH. As a preferred scheme, the kit further includes a metal ion that competes for ferrous ions. The invention provides a method for detecting the contentof the alpha-oxoglutarate with high efficiency and high precision, which can be used for detecting the enzymatic activity of alpha-oxoglutarate dependent enzyme, determining the enzyme kinetic parameters, and performing high-throughput drug screening by targeting the type of enzyme. The method solves the problems of complex operation, low accuracy, reproducibility, low flux, and difficulty in screening high-throughput drugs for the enzyme activity detection of the alpha-oxoglutarate dependent enzyme.
Owner:SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
Isoxazole derivative as mutated isocitrate dehydrogenase 1 inhibitor
ActiveCN106795146AStrong activity inhibitionInhibitionOrganic active ingredientsOrganic chemistryIDH1Limonoate dehydrogenase
It was discovered that a compound of general formula (I) that has an isoxazole skeleton has an excellent inhibitory activity on a mutated IDH1 protein, inhibits 2-HG production by the aforesaid protein and can effectively inhibit the proliferation of various tumors expressing the aforesaid protein. In general formula (I), R1, R2, R3, Y and Z are each as defined in claim 1.
Owner:DAIICHI SANKYO CO LTD +1
A kind of L-carnitine-producing Escherichia coli genetically engineered bacteria and its construction method and application
ActiveCN104928225BHigh copy numberEnhance and stabilize expressionBacteriaMicroorganism based processesBiotechnologyIsocitrate Dehydrogenase (NAD+)
Owner:武汉中科光谷绿色生物技术有限公司
Isoxazole derivatives as mutant isocitrate dehydrogenase 1 inhibitors
ActiveCN106795146BInhibitionStrong activity inhibitionOrganic active ingredientsOrganic chemistryIDH1Isocitrate Dehydrogenase (NAD+)
Owner:DAIICHI SANKYO CO LTD +1
Ammonia (ammonia ion) measuring method and ammonia (ammonia ion) diagnosing/measuring kit
InactiveCN102565375AMicrobiological testing/measurementColor/spectral properties measurementsIsocitrate Dehydrogenase (NAD+)Pyruvate synthesis
The invention relates to a method for measuring ammonia (ammonia ion) content through an enzymatic colorimetric method and an enzyme coupling reaction technique, and the composition and the constituents of a reagent. The measuring technical principle of the method is fulfilled through the serial catalytic reactions of ammonia kinase, pyruvate carboxylase, and isocitrate dehydrogenase. The invention further relates to an ammonia (ammonia ion) diagnosing / measuring kit. The method provided by the invention has the advantages of high sensitivity and small error, so that the method and the kit provided by the invention can be widely applied to clinical medicine / food inspections.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Glycine Determination Method and Glycine Determination Kit
InactiveCN102298021AMicrobiological testing/measurementColor/spectral properties measurementsGlycineGlutamate decarboxylase
The invention relates to a method for measuring glycine content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the series of glycine transaminase, glutamic acid decarboxylase and isocitrate dehydrogenase After the catalytic reaction is completed, the present invention also relates to a glycine assay kit. The determination method of the invention has high sensitivity and small error, so the determination method and the kit of the invention can be widely used in food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Utilize electrical energy to fix co 2 And engineering bacteria for synthesizing isopropanol and construction method
ActiveCN114561416BEfficient conversionBacteriaMicroorganism based processesBiotechnologyIsocitrate Dehydrogenase (NAD+)
The present invention provides a method for designing and constructing high-efficiency use of electrical energy to fix CO by using synthetic biotechnology. 2 And the method and application of engineering bacteria for synthesizing isopropanol. The method of designing and constructing engineering bacteria is to be able to utilize electric energy and CO. 2 electroactive microorganisms Geobacter sulfurreducens ACL is the chassis. In the chassis strain, isocitrate dehydrogenase and isopropanol synthesis pathway-related enzymes are efficiently expressed by plasmids. It has been verified by experiments that the strain constructed by this method can efficiently utilize electricity and CO. 2 Synthesis of isopropanol.
Owner:深圳中科翎碳生物科技有限公司
Method and kit for measuring glycine
InactiveCN102087291AMicrobiological testing/measurementColor/spectral properties measurementsGlycineEnzymatic Colorimetry
The invention relates to a method for measuring glycine content by utilizing a double amplification method, an enzyme colorimetric method and an enzyme linked method technology, and composition and components of reagents. The measuring technical principle is that the measurement is finished according to a series of catalytic reactions of glycine dehydrogenase, isocitrate lyase and isocitrate dehydrogenase. The invention also relates to a kit for measuring glycine. The measuring method has high sensitivity and small error; therefore, the measuring method and the kit can be widely applied to food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Homocysteine diagnosing/measuring reagent (kit) and homocysteine concentration measuring method
InactiveCN101750393AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsChemistryIsocitrate dehydrogenase
The invention relates to a homocysteine diagnosing / measuring reagent (kit) using the technologies of an enzyme colorimetric method and an enzyme linkage method. At the same time, the invention also relates to a homocysteine concentration measuring method, the reagent composition and reagent ingredients, which belong to the technical field of medical detection and measurement. The reagent (kit) mainly comprises the following ingredients: a buffer solution, reduced form cozymase, cozymase A, oxidized form ferredoxin, 2-oxoglutarate, homocysteine desulfhydrase, 2-oxobutyryl synthetase, isocitrate dehydrogenase and stabilizing agents. The method has the following steps: mixing samples and the reagent by the certain volume percent for making the samples and the reagent take a series of enzymatic reaction, and then, placing reactants under an ultraviolet / visible light analyzer to detect the dropping degree of the light absorbancy in the position with the main wave length of 340 nm, so the concentration of the homocysteine can be measured and calculated.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Ammonia (ammonium ion) determination method and ammonia (ammonium ion) diagnosis/determination kit
InactiveCN102565358AMicrobiological testing/measurementColor/spectral properties measurementsIsocitrate Dehydrogenase (NAD+)Propionyl-CoA carboxylase
The invention relates to an ammonia (ammonium ion) content determination method through utilizing enzymatic colorimetry and an enzyme-linked immunosorbant assay technology, and the composition and components of a reagent for the determination. According to the determination technology principle, the determination is completed according to a series of catalytic reactions of an ammonia kinase, a propionyl CoA carboxylase and an isocitrate dehydrogenase. The invention also relates to an ammonia (ammonium ion) diagnosis / determination kit. The determination method of the invention has the advantages of high sensitivity and small error, so the determination method and the kit of the invention can be widely applied to clinical medicine / food examination.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Isocitrate dehydrogenase (IDH) inhibitors
PendingCN114555597AOrganic active ingredientsOrganic chemistryDiseaseIsocitrate Dehydrogenase (NAD+)
Owner:ZHEJIANG METON PHARM CO LTD
Genetic and biochemical locus markers of inbred gerbils with cerebral ischemia and their application
ActiveCN107870190BEasy to operateFast intuitive homozygosityMaterial analysis by electric/magnetic meansAmylaseIsocitrate Dehydrogenase (NAD+)
The invention relates to a genetic biochemical site marker for cerebral ischemia inbred line meriones unguiculatus and application of the biochemical site marker. The genetic biochemical site marker for the cerebral ischemia inbred line meriones is selected from at least one of glucose-6-phosphate dehydrogenase-1, esterase-3, carbonic anhydrase-2, alkaline phosphatase-1, isocitrate dehydrogenase-1, malic enzyme-1, glucose phosphate translocase-1, renal catalase-2, peptidase-3, glucose phosphate isomerase-1, hemoglobin-beta chain, transferring, esterase-1, esterase-10, glycerol phosphate dehydrogenase-1, beta-glucuronidase-1, esterase-2, lactic dehydrogenase regulator-1, serum protein-1, amylase-1, esterase-6, esterase-8, esterase-9, esterase-4, catalase-1 and esterase-12.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Total bilirubin determination kit preparation method
PendingCN112986584APromote oxidation reactionPromote autoxidationBiological testingIsocitrate Dehydrogenase (NAD+)Active agent
The invention is applicable to the technical field of kit preparation, and provides a total bilirubin determination kit preparation method, which comprises the steps of: A, preparing a reagent 1, the reagent 1 comprising a citric acid buffer solution, a surfactant and triton, the components of the citric acid buffer solution comprising isocitrate dehydrogenase, lactic dehydrogenase, a stabilizer and a coenzyme; B, preparing a single reagent, wherein the single reagent comprises disodium hydrogen phosphate, monopotassium phosphate, sodium vanadate and disodium ethylene diamine tetraacetate; C, uniformly stirring and mixing the citric acid buffer solution and the single reagent, keeping the temperature at 37 DEG C for 3-10 minutes, adding the enzyme reagent, and uniformly mixing to obtain a reaction solution; and D, pouring the reaction liquid into a kit, wherein a substrate reagent is carried in the kit, and the substrate reagent comprises an oxidase substrate and a substrate buffer solution. Therefore, the influence of lipid turbidity on detection can be eliminated, so that the detection accuracy is improved.
Owner:潍坊泽成生物技术有限公司
Determination method of ammonia (ammonium ion) and diagnosis/determination reagent kit of ammonia (ammonium ion)
InactiveCN102539687AMicrobiological testing/measurementColor/spectral properties measurementsEnzymatic ColorimetryCoupling reaction
The invention relates to a determination method for determining ammonia (ammonium ion) content by utilizing an enzymatic colorimetric method and enzyme couple reaction technology, and composition and components of a reagent. The technology principle for the determination is that the determination is finished according to series catalytic reactions of ammonia kinase, biotin carboxylase and isocitrate dehydrogenase. The invention further relates to a diagnosis / determination reagent kit of ammonia (an ammonium ion). The determination method is high in sensitivity and small in errors, and accordingly the determination method and the reagent kit can be widely applied to clinical medicine / food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Glycine Determination Method and Glycine Determination Kit
InactiveCN102298031AMicrobiological testing/measurementColor/spectral properties measurementsChemistryIsocitrate dehydrogenase
The invention relates to a method for measuring glycine content using enzyme colorimetry and enzyme-linked method technology, the composition and components of reagents, and the technical principle of its measurement is based on the series of glycine transaminase, glutamic acid oxidase and isocitrate dehydrogenase After the catalytic reaction is completed, the present invention also relates to a glycine assay kit. The determination method of the invention has high sensitivity and small error, so the determination method and the kit of the invention can be widely used in food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Detecting method of ammonia (ammonium ion) and ammonia (ammonium ion) diagnosing/detecting kit
InactiveCN102565351AMicrobiological testing/measurementColor/spectral properties measurementsAdditive ingredientCarbamyl Phosphate
The invention relates to a detecting method of ammonia (ammonium ion) content by utilizing an enzymatic colorimetry and an enzyme-linked method, and composition and ingredients of reagents. The technology principle of detection is that the detection is completed according to the serial catalytic reaction of ammonia kinase, carbamyl phosphate synthetase and isocitrate dehydrogenase. The invention also relates to an ammonia (ammonium ion) diagnosing / detecting kit. The detecting method has high sensitivity and small error, so that the detecting method and the kit can be widely applied to clinical medical / food inspection.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
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