30 results about "Propionyl-CoA" patented technology

Methods and cells for producing scattered branched-chain fatty acids are provided. For example, the invention provides a method for producing branched-chain fatty acid comprising a methyl on one or more even number carbons. The method comprises culturing a cell comprising an exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes the conversion of propionyl-CoA to methylmalonyl-CoA and / or an exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes the conversion of succinyl-CoA to methylmalonyl-CoA, under conditions allowing expression of the polynucleotide(s) and production of branched-chain fatty acid. The cell produces more branched-chain fatty acid comprising a methyl on one or more even number carbons than an otherwise similar cell that does not comprise the polynucleotide(s). A cell that produces branched-chain fatty acid and the branched-chain fatty acid also are provided.

The invention provides fermentative methods for producing n-propanol. The methods of the invention involve providing a suitable carbon source, a microorganism expressing the dicarboxylic acid pathway, reducing equivalents, and at least one gene coding for an enzyme that catalyzes the conversion of propionate / propionyl-CoA into n-propanol. The methods further involve contacting the carbon source and reducing equivalents with the microorganism under conditions favorable for the production of n-propanol. Also provided are methods for producing propylene and polypropylene from the n-propanol and microorganisms suitable for use in the methods of the invention.

The invention discloses recombination klebsiella pneumonia capable of co-producing 3-HP and P3HP, and a preparation method and application thereof. The recombination bacterium is obtained by introducing glycerol dehydratase gene, glycerol dehydratase reactivation enzyme gene, aldehyde dehydrogenase gene, propionyl coenzyme A synthetase gene and polyhydroxyalkanoate synthetase gene into a host recombination klebsiella pneumonia in which 1, 3-propylene glycol oxidoreductase gene and aldehyde reductase / alcohol dehydrogenase gene are knocked out. According to the technical scheme, the production cost of 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) is reduced, and 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) can be synthesized at the same time by taking a same thallus as the host.

The present invention relates to a method for treating and / or preventing Huntington disease and other polyglutamine diseases, comprising the step of administering an effective amount of a precursor of propionyl-CoA to an individual in need thereof.

The present invention relates to a method for treating and / or preventing Huntington disease and other polyglutamine diseases, comprising the step of administering an effective amount of a precursor of propionyl-CoA to an individual in need thereof.

Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.

The invention discloses recombination klebsiella pneumonia capable of co-producing 3-HP and P3HP, and a preparation method and application thereof. The recombination bacterium is obtained by introducing glycerol dehydratase gene, glycerol dehydratase reactivation enzyme gene, aldehyde dehydrogenase gene, propionyl coenzyme A synthetase gene and polyhydroxyalkanoate synthetase gene into a host recombination klebsiella pneumonia in which 1, 3-propylene glycol oxidoreductase gene and aldehyde reductase / alcohol dehydrogenase gene are knocked out. According to the technical scheme, the production cost of 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) is reduced, and 3-hydroxypropionic acid and poly(3-hydroxypropionic acid) can be synthesized at the same time by taking a same thallus as the host.

A microorganism capable of producing acrylic acid, comprising a genetic modification that increases activity of CoA acylating aldehyde dehydrogenase (ALDH) catalyzing conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxy propionyl-CoA (3-HP-CoA) and a genetic modification that increases activity of 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA in the microorganism in comparison with a cell that is not genetically engineered; as well as a method of producing the microorganism, and a method of producing acrylic acid using the same.

The invention discloses a Corynebacterium glutamicum and application thereof, belonging to the field of microorganisms. The Corynebacterium glutamicum was preserved in the China Center for Type Culture Collection on June 1, 2016, and the preservation number is CCTCC NO: M 2016303. The Corynebacterium glutamicum can accumulate more propionyl-CoA in cells, which is 15.8 times higher than that of ATCC13869, and can be used for the production of related metabolites using propionyl-CoA as a substrate. After the polyhydroxyalkanoate synthesis gene is introduced, the Corynebacterium glutamicum can synthesize PHBV, but the synthetic product is PHB in ATCC13869. The invention solves the current situation of producing PHBV by adding propionate or propionate from an exogenous source, realizes the direct production of PHBV in Corynebacterium glutamicum without adding propionate or propionate from an exogenous source, and reduces production costs.

The invention belongs to the technical field of bioengineering, and particularly discloses a new method for producing high-yield ethyl lactate by using a saccharomyces cerevisiae strain and application of the strain. High-yield ethyl lactate is produced through the simultaneous heterologous expression of lactate dehydrogenase, propionyl-CoA transferase and alcohol acyltransferase in saccharomycescerevisiae. Pyruvate decarboxylase is completely knocked out from an original strain, and lactate dehydrogenase is overexpressed to obtain the yeast strain with a certain capability of generating L-lactic acid; a strong promoter TEF1 is selected and adopted for overexpressing propionyl-CoA transferase, a strong promoter PGK1 is selected and adopted for overexpressing alcohol acyltransferase, witha homothallic gene HO as an integration site, a copy number of propionyl-CoA transferase is increased, the saccharomyces cerevisiae strain capable of significantly increasing the yield of ethyl lactate is obtained, and the yield of ethyl lactate obtained by using the saccharomyces cerevisiae strain reaches 353.1 mg / L; compared with the original strain, the yield of isoamyl alcohol is decreased by49.9%, the yield of phenylethyl alcohol is decreased by 59.2%, and the new method meets the higher requirements of yeast in the related field of liquor and has broad application prospects.

Provided are a recombinant microorganism capable of producing polylactate (PLA) or hydroxyalkanoate-lactate copolymers and a method of preparing PLA or hydroxyalkanoate-lactate copolymers using the same. The recombinant microorganism has both a gene encoding a propionyl-CoA transferase from Megasphaera elsdenii and a gene encoding a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate. A propionyl-CoA transferase from Megasphaera elsdenii is introduced into the recombinant microorganism to effectively provide lactyl-CoA, thereby enabling efficient preparation of PLA or PLA copolymers.

The present invention relates to the treatment and prevention of movement disorders with the administration of one or more propionyl-CoA precursors.

The invention relates to diaryl propionyl-N-methyl hydroxamic acid compounds which have a structural general formula shown in the specification. The diaryl propionyl-N-methyl hydroxamic acid compounds have a good function of inhibiting urease, and can be used for preparing medicaments for resisting gastritis, gastric ulcer, lithangiuria and the like. The invention also discloses a method for preparing the diaryl propionyl-N-methyl hydroxamic acid compounds.

The present invention provides a hydroxyacyl-coenzyme A dehydrogenase gene, an acyl-coenzyme A thiolase gene, genetically engineered strains and a use thereof. The hydroxyacyl-coenzyme A dehydrogenase gene encodes a protein (i) or (ii) as follows: (i) having an amino acid sequence according to SEQ ID NO 2; (ii) derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by (i) and having the same function as that of the protein of (i). The present invention constructs genetically engineered Mycobacterium strains lacking of a hydroxyacyl-coenzyme A dehydrogenase gene or an acyl-coenzyme A thiolase gene, which are used in the preparation of steroidal compounds, such as 1,4-BNA, 4-BNA, 9-OH-BNA, etc. Further, the invention improves the production efficiency and product quality of steroidal drug, improves the utilization of drug precursors, reduces the production costs, and provides the advantages of mild reaction conditions, environmentally friendly, and high economic and social benefits.

The invention provides recombinant bacteria for synthesizing succinic acid from a fixed amount of carbon dioxide, as well as a construction method and application of the recombinant bacteria, and belongs to the field of gene engineering and fermentation engineering. A recombinant bacteria host is escherichia coli, which can express a gene accADBC of acetyl-CoA carboxylase, a gene mcr of malonyl CoA reductase, a gene pcs of propionyl CoA synthase, a gene pcc of propionyl CoA carboxylase, a gene mcE of methylmalonyl-CoA isomerase, a gene mcM of methylmalonyl-CoA mutase and a gene sucCD of succinyl-coenzyme A synthase. According to the construction method, recombinant carriers pACYCDuet-accADBC-pcs and pETDuet-mcr-pcc-sucCD-mcEM are transformed into a host cell. The recombinant bacteria constructed through the construction method can adopt glucose serving as a unique carbon source to synthesize the succinic acid, and fermentation is performed for 48 hours, thus obtaining the succinic acid with the concentration of 6.06 g / L.

The invention relates to diaryl propionyl-N-methyl hydroxamic acid compounds which have a structural general formula shown in the specification. The diaryl propionyl-N-methyl hydroxamic acid compounds have a good function of inhibiting urease, and can be used for preparing medicaments for resisting gastritis, gastric ulcer, lithangiuria and the like. The invention also discloses a method for preparing the diaryl propionyl-N-methyl hydroxamic acid compounds.

The present invention discloses a method for treating a subject suffering from intermittent claudication (IC) wherein said subject takes an effective dose of propionyl L-carnitine or a pharmaceutically acceptable salt thereof and also engaging in a concomitant physical training program. The method is particular effective for patients with intermittent claudication at class II of the Leriche-Fontaine's classification. The method provides a relief from peripheral arterial disease symptoms, as shown by claudication-limited exercise tolerance, while also improving quality of life.