Total bilirubin determination kit preparation method

Abstract

The invention is applicable to the technical field of kit preparation, and provides a total bilirubin determination kit preparation method, which comprises the steps of: A, preparing a reagent 1, the reagent 1 comprising a citric acid buffer solution, a surfactant and triton, the components of the citric acid buffer solution comprising isocitrate dehydrogenase, lactic dehydrogenase, a stabilizer and a coenzyme; B, preparing a single reagent, wherein the single reagent comprises disodium hydrogen phosphate, monopotassium phosphate, sodium vanadate and disodium ethylene diamine tetraacetate; C, uniformly stirring and mixing the citric acid buffer solution and the single reagent, keeping the temperature at 37 DEG C for 3-10 minutes, adding the enzyme reagent, and uniformly mixing to obtain a reaction solution; and D, pouring the reaction liquid into a kit, wherein a substrate reagent is carried in the kit, and the substrate reagent comprises an oxidase substrate and a substrate buffer solution. Therefore, the influence of lipid turbidity on detection can be eliminated, so that the detection accuracy is improved.

Description

technical field [0001] The invention relates to the technical field of kit preparation, in particular to a preparation method of a total bilirubin assay kit. Background technique [0002] Total bilirubin is the general term for conjugated and unconjugated bilirubin produced by heme metabolism in the human body. Abnormally high total bilirubin is often seen in liver and gallbladder diseases. In recent years, some studies have shown that bilirubin in blood is lower than normal Often associated with coronary heart disease. Therefore, the determination of total bilirubin in human serum, plasma or urine is one of the most routine items in medical testing. The reagent is at about PH3.00, in the presence of surfactants and accelerators, the total bilirubin in the sample is oxidized to biliverdin by the oxidant sodium vanadate, the change before and after the absorbance is measured, and the total bilirubin in the sample is calculated. content. [0003] For the determination of bi...

AI Technical Summary

Problems solved by technology

[0003] For the determination of bilirubin, the diazonium reagent method is traditionally used. This method has a long history and is more accurate.

Enzymatic determination of total bilirubin has specific characteristics, but its kit is not conducive to popularization and application due to poor thermal stability of the enzyme, short storage time, and high price

[0004] Existing kits are inaccurate when testing samples with severe lipid turbidity

At present, people's diet is high in fat content, and the blood is not necessarily fasted overnight, so the probability of fat turbidity in serum samples is also increasing, resulting in a high sample blank and affecting the determination of bilirubin

[0005] In summary, there are obviously inconveniences and defects in the actual use of the existing technology, so it is necessary to improve