The invention relates to the technical field of detection of ischemic modified albumin, in particular to a detection reagent for ischemic modified albumin. The reagent R1 contains buffer, cobalt chloride, dodecyl dimethyl betaine and preservatives; the reagent R2 Contains buffer, thiadiazole azodimethylaminophenol, lauryldimethylbetaine, preservatives. The use of a new chromogenic agent, thiadiazole azodimethylaminophenol, effectively avoids the interference of metal ions such as Pd, Ni, Zn, and Cu, and is more stable, low-toxic, and easy to store, greatly enhancing the stability of the reagent; using a new The amphoteric surfactant, dodecyl dimethyl betaine, not only significantly improves the performance of the assay, but also maintains the stability of the system and prevents fatty turbidity. The effect is obvious, and the stability and anti-interference ability of the reagent are significantly improved.