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Anti-heparan-interference ischemia modified albumin detection reagent

A technology for detecting reagents and albumin, which is applied in the field of ischemia-modified albumin detection, to achieve obvious effects, prevent white turbidity, and improve performance

Active Publication Date: 2014-02-05
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of poor response curve, poor correlation and poor accuracy in the above detection of heparin samples, the present invention provides a Ischemia-modified albumin detection reagent with excellent response curve, correlation and accuracy against heparin interference

Method used

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  • Anti-heparan-interference ischemia modified albumin detection reagent
  • Anti-heparan-interference ischemia modified albumin detection reagent
  • Anti-heparan-interference ischemia modified albumin detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: An Existing Ischemia-Modified Albumin Detection Reagent

[0041] The weight percentage of each component in the reagent 1:

[0042] Deionized water 88.9%,

[0043] Tris Buffer (Tris) 10%,

[0044] CoCl 2 6H2O 0.1%,

[0045] NaN3 1%,

[0046] After mixing the above ingredients, it is reagent 1 (cobalt ion reagent), and adjust the pH value to 7.5 with HCl or NaOH;

[0047] The weight percentage of each component in the reagent 2:

[0048] Deionized water 88%,

[0049] Tris Buffer (Tris) 10%,

[0050] DTT 1%,

[0051] NaN3 1%,

[0052] After mixing the above ingredients, it is reagent 2 (thiothreitol reagent), and adjust the pH value to 5.9 with HCl or NaOH.

Embodiment 2

[0053] Example 2: Anti-heparin interference detection reagent for ischemia-modified albumin of the present invention

[0054] The weight percentage of each component in the reagent 1:

[0055] Deionized water 88.7%,

[0056] Tris Buffer (Tris) 10%,

[0057] CoCl 2 6H2O 0.1%,

[0058] NaN3 1%,

[0059] Alkyl glycoside APG1214 0.1%,

[0060]Emulgen709 0.1%,

[0061] After mixing the above ingredients, it is reagent 1 (cobalt ion reagent), and adjust the pH value to 7.5 with HCl or NaOH;

[0062] The weight percentage of each component in the reagent 2:

[0063] Deionized water 88%,

[0064] Tris Buffer (Tris) 10%,

[0065] DTT 1%,

[0066] NaN3 1%,

[0067] After mixing the above ingredients, it is reagent 2 (thiothreitol reagent), and adjust the pH value to 5.9 with HCl or NaOH.

[0068] 2) The method of using the reagents in this example:

[0069] For the anti-heparin interference IMA detection reagent described in this example, 20 fresh clinical samples were ra...

Embodiment 3

[0082] Example 3: Ischemia-modified albumin detection reagent against heparin interference

[0083] 1) Weight percentage of each component in reagent 1:

[0084] Deionized water 86.9%

[0085] Tris Buffer (Tris) 10%

[0086] CoCl 2 6H2O 0.1%

[0087] NaN3 1%

[0088] Alkyl Glycoside APG1214 1%

[0089] Emulgen709 1%

[0090] After mixing the above ingredients, it is reagent 1 (cobalt ion reagent), and adjust the pH value to 7.5 with HCl or NaOH.

[0091] 2) Weight percentage of each component in reagent 2:

[0092] Deionized water 88%

[0093] Tris Buffer (Tris) 10%

[0094] DTT 1%

[0095] NaN3 1%

[0096] After mixing the above ingredients, it is reagent 2 (thiothreitol reagent), and adjust the pH value to 5.9 with HCl or NaOH.

[0097] 2) This example describes the ability of the IMA reagent to resist heparin interference after increasing the weight percentage of the alkyl glycoside APG1214 and Emulgen709 from 0.1% to 1% at the same time;

[0098] 3) Its mode...

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Abstract

The invention relates to the technical field of ischemia modified albumin detection and in particular relates to an anti-heparan-interference ischemia modified albumin detection reagent. The reagent comprises a reagent 1 and a reagent 2, wherein the reagent 1 contains 0.1-1 percent of alkyl polyglycoside (APG) 1214 and 0.1-1 percent of Emulgen709. Two special nonionic surfactants, namely the APG1214 and the Emulgen709 are added into the reagent 1, so that the measurement performance is obviously improved, and the effects of maintaining the transparency of the system and preventing white turbidity are obvious. Compared with previously used Tween-80, Tritonx-100 and polyethylene glycol monooleyl ether, the two special nonionic surfactants have better effects, and the heparan interference resistance of the detection reagent is obviously improved.

Description

technical field [0001] The invention relates to the technical field of ischemia-modified albumin detection, in particular to a heparin-resistant ischemia-modified albumin detection reagent. Background technique [0002] Ischemia-modified albumin (IMA) is a biomarker for the diagnosis of acute coronary syndrome (ACS). ACS is characterized by acute onset, rapid changes, uneven clinical manifestations and risks, and early diagnosis is difficult. Traditional biomarkers only increase when myocardial necrosis occurs, but at this time irreversible pathological damage has been brought to the patient. Therefore, there is an urgent need for an early sensitive and strong biochemical index that can reflect myocardial ischemia for early diagnosis, and IMA is a research hotspot in recent years, which has opened up a new way for the research of early diagnosis of ACS. [0003] IMA detection is based on myocardial ischemia can cause changes in the N-terminal of human serum albumin (huma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803G01N33/683G01N2800/325
Inventor 谭柏清罗维晓甘宜梧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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