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Ischemia modified albumin liquid stabilization kit

A kit and albumin technology, which is applied in the field of medical testing and determination, can solve the problems of short stability time of aqueous solution refrigeration or cryopreservation, affecting the large-scale clinical application of reagents, and the validity period of only 2 weeks, meeting the requirements of clinical testing, The effect of reducing the rate of oxidation and improving stability

Active Publication Date: 2012-06-20
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the kit can be applied to Roche’s COBAS MIRA?Plus, COBAS INTEGRA?700 / 800, Hitachi?917 and Roche / Hitachi Modular P, Beckman Coulter Synchron LX?20 and other instruments, but the price is expensive, and it is a dry powder reagent, and the validity period after reconstitution is only 2 weeks
Since dithiothreitol is used as the chromogenic agent in IMA determination, the stability time of its aqueous solution in cold storage or frozen storage is relatively short. In addition, because dithiothreitol can chelate with other heavy metals in the sample and affect the accuracy of the determination, it is serious Affected the large-scale clinical application of the reagent

Method used

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  • Ischemia modified albumin liquid stabilization kit
  • Ischemia modified albumin liquid stabilization kit
  • Ischemia modified albumin liquid stabilization kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The components and concentrations of reagent 1 (R1) are:

[0039] Tris buffer (pH 7.5) 0.1 M

[0040] Cobalt chloride 50 mg / L

[0041] Tween 20 0.1 g / L

[0042] Sodium Fluoride 0.1 g / L

[0043] Sodium azide 0.1 g / L

[0044] The components and concentrations of reagent 2 (R2) are:

[0045] Citric acid-trisodium citrate buffer (pH 7.0) 1 M

[0046] Dithiothreitol 0.5 g / L

[0047] Bridger-35 1 g / L

[0048] Sodium azide 0.1 g / L

[0049] The preparation method of Reagent 1 and Reagent 2 is a conventional method, that is, the components of Reagent 1 and Reagent 2 are added to distilled water and then mixed and stirred evenly.

Embodiment 2

[0051] The components and concentrations of reagent 1 are:

[0052] 3-Trimethylolmethylamine-2-hydroxypropanesulfonic acid buffer (pH 8.0) 0.2 M

[0053] Cobalt chloride 20 mg / L

[0054] Sodium Fluoride 5 g / L

[0055] Citric acid 0.7 g / L

[0056] Tween 20 1 g / L

[0057] Sodium azide 1 g / L

[0058] The components and concentrations of reagent 2 are:

[0059] Acetic acid-sodium acetate buffer (pH 7.5) 2 M

[0060] Dithiothreitol 10 g / L

[0061] Tween 80 5 g / L

[0062] Proclin300 0.1 g / L

[0063] The preparation method of the kit in Example 2 is the same as in Example 1.

Embodiment 3

[0065] The components and concentrations of reagent 1 are:

[0066] 4-Hydroxyethylpiperazine propanesulfonic acid buffer (pH 8.5) 0.2 M

[0067] Cobalt chloride 200 mg / L

[0068] Glycine 2.5 g / L

[0069] Tween 80 2 g / L

[0070] Proclin300 1g / L

[0071] The components and concentrations of reagent 2 are:

[0072] Imidazole buffer (pH 8.0) 10 M

[0073] Dithiothreitol 30 g / L

[0074] Triton 100 1 g / L

[0075] Proclin300 1g / L

[0076] Example 3 The preparation method of the kit is the same as that in Example 1.

[0077] The testing conditions for the kit of the present invention to measure IMA in samples are as follows: temperature: 30-37° C.; optical path of cuvette: 1.0 cm. The main detection wavelength is 510 nm, and the secondary wavelength is 700 nm.

[0078] The method of using the IMA determination kit of the present invention to determine the IMA in the sample is as follows: add R1 to the sample (calibration tube as the sample) and mix well, incubate at 30-37°C ...

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Abstract

The invention discloses an ischemia modified albumin liquid stabilization kit. The kit is a liquid dual reagent of a reagent 1 and a reagent 2, wherein the reagent 1 comprises a buffer solution of which the pH is 7.5 to 8.5 and the concentration is 0.05 to 0.5M, cobalt chloride at the concentration of 20 to 200mg / L, an interference removing agent at the concentration of 0.1 to 10g / L, a stabilizing agent at the concentration of 0.1 to 10g / L, and preservative at the concentration of 0.1 to 10g / L; and the reagent 2 comprises a buffer solution of which the pH is 7.0-8.0 and the concentration is 0.5 to 10M, dithiothreitol at the concentration of 0.5 to 50g / L, a stabilizing agent at the concentration of 0.1 to 10g / L, and preservative at the concentration of 0.1 to 10g / L. The kit has the advantages of fully meeting the clinical inspection requirement, along with high accuracy and stability, and can be stabilized for 12 months when stored at the temperature of between 2 and 8 DEG C in a dark place.

Description

Technical field [0001] The invention is a medical test and measurement technology field, which involves a kind of ischemic modified albumin liquid stabilization kit. Background technique [0002] Acute coronary syndrome (Acute Coronary Syndromes (ACS) has different clinical manifestations, but all have the same pathological process, namely atherosclerotic plaque, platelet aggregation, and thrombosis, which causes coronary arteries to narrow and obstruct.Causes myocardial ischemia. If the longitudinal ischemia time is prolonged, myocardial cell necrosis and myocardial damage.Therefore, it is essential for patients to identify myocardial ischemia before myocardial necrosis, and the diagnosis and starting treatment as soon as possible are essential.The current diagnosis of heart ischemia depends on the changes of the ST segment and T wave of the ECG. This is the easiest, fastest, and cheapest method of myocardial ischemia.Its sensitivity is less than 50%.The acute myocardial infusio...

Claims

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Application Information

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IPC IPC(8): G01N33/52
Inventor 邹炳德沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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