Steady ischemia modified albumin kit
An albumin and kit technology, applied in the field of ischemia-modified albumin kits, can solve the problems of short half-life solution, poor stability, oxidation, etc., and achieve the effects of improving stability and stable test performance.
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Embodiment 1
[0033] The stable ischemia-modified albumin kit in this example is composed of cobalt ion reagent and dithiothreitol reagent;
[0034] Among them, the components and concentrations of the cobalt ion reagent are: triethanolamine buffer solution: 100mmol / L, cobalt chloride: 0.2g / L, TritonX-100: 1g / L, sodium azide: 0.5g / L, wherein, The pH value of triethanolamine buffer solution is 8.0;
[0035] The components and concentrations of dithiothreitol reagent are: 2-morpholineethanesulfonic acid buffer: 100mmol / L, DTT: 1.0g / L, TritonX-100: 1g / L, mannitol: 5-20g / L L, sodium azide: 0.5g / L, wherein, the pH value of the 2-morpholineethanesulfonic acid buffer is 5.5.
Embodiment 2
[0037] The stable ischemia-modified albumin kit in this example is composed of cobalt ion reagent and dithiothreitol reagent;
[0038] Among them, the components and concentrations of the cobalt ion reagent are: triethanolamine buffer solution: 100mmol / L, cobalt chloride: 0.2g / L, TritonX-100: 1g / L, sodium azide: 0.5g / L, wherein, The pH value of triethanolamine buffer solution is 8.0;
[0039] The components and concentrations of dithiothreitol reagent are: 2-morpholineethanesulfonic acid buffer: 100mmol / L, DTT: 1.0g / L, TritonX-100: 1g / L, glucose: 10g / L, Sodium nitrogen: 0.5g / L, wherein the pH value of the 2-morpholineethanesulfonic acid buffer is 5.5.
Embodiment 3
[0041] The stable ischemia-modified albumin kit in this example is composed of cobalt ion reagent and dithiothreitol reagent;
[0042] Among them, the components and concentrations of the cobalt ion reagent are: triethanolamine buffer solution: 100mmol / L, cobalt chloride: 0.2g / L, TritonX-100: 1g / L, sodium azide: 0.5g / L, wherein, The pH value of triethanolamine buffer solution is 8.0;
[0043] The components and concentrations of dithiothreitol reagent are: 2-morpholineethanesulfonic acid buffer: 100mmol / L, DTT: 1.0g / L, TritonX-100: 1g / L, glycerol: 10g / L, Sodium nitrogen: 0.5g / L, wherein the pH value of the 2-morpholineethanesulfonic acid buffer is 5.5.
[0044] figure 1 It is the calibration curve of the calibrator with a concentration of 70.6U / ml for the stable ischemia-modified albumin kit of the present invention, figure 1 The reading points in are every 34 points for 10 minutes. from figure 1It can be seen that the kit of the present invention can quickly reach the en...
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