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Ischemia modified albumin detection reagent and detection method thereof

A detection reagent and technology for albumin, applied in the field of ischemia-modified albumin detection, can solve the problems of interference, poor reproducibility and specificity, and achieve easy storage, good accuracy and stability, and improved stability. and the effect of anti-interference ability

Active Publication Date: 2015-06-10
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of poor reproducibility and specificity and serious interference in the detection of ischemic modified albumin in the above prior art, the present invention provides A high sensitivity, good anti-interference performance, stable ischemia-modified albumin detection reagent

Method used

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  • Ischemia modified albumin detection reagent and detection method thereof
  • Ischemia modified albumin detection reagent and detection method thereof
  • Ischemia modified albumin detection reagent and detection method thereof

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Embodiment 1

[0032] Detection reagents for ischemia-modified albumin, including reagent R1 and reagent R2:

[0033] 1) The composition of its R1 is:

[0034] PIPES (piperazine-1,4-diethanesulfonic acid) buffer (pH=7.6, 25°C) ·························· 0.1mol / L

[0035] CoCl 2 (Cobalt chloride) ······················································································· 24mmol / L

[0036] Lauryl dimethyl betaine ··········································································· 3g / L

[0037] Preservative NaN 3···························································································· 0.5g / L

[0038] 2) The components of reagent R2 are:

[0039] MES buffer (pH=5.0, 25°C) ································································· 0.1mol / L

[0040] Thiadiazole azodimethylaminophenol (TDAMAP) ················································· 24mmol / L

[0041] Lauryl dimethyl betaine ·······················································...

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Abstract

The invention relates to the technical field of detection of ischemic modified albumin, in particular to a detection reagent for ischemic modified albumin. The reagent R1 contains buffer, cobalt chloride, dodecyl dimethyl betaine and preservatives; the reagent R2 Contains buffer, thiadiazole azodimethylaminophenol, lauryldimethylbetaine, preservatives. The use of a new chromogenic agent, thiadiazole azodimethylaminophenol, effectively avoids the interference of metal ions such as Pd, Ni, Zn, and Cu, and is more stable, low-toxic, and easy to store, greatly enhancing the stability of the reagent; using a new The amphoteric surfactant, dodecyl dimethyl betaine, not only significantly improves the performance of the assay, but also maintains the stability of the system and prevents fatty turbidity. The effect is obvious, and the stability and anti-interference ability of the reagent are significantly improved.

Description

technical field [0001] The invention relates to the technical field of detection of ischemia-modified albumin, in particular to a detection reagent of ischemia-modification albumin and a detection method using the detection reagent. Background technique [0002] Ischemia-modified albumin (IMA) is due to the amino acid sequence of serum albumin being damaged by free radicals during ischemia, resulting in changes in the ability to bind transition metals. Ischemia-modified albumin is mainly used for auxiliary diagnosis of acute myocardial ischemia, and is of great significance for excluding acute coronary syndrome (ACS) such as acute myocardial infarction and unstable angina. It is an ideal new type of drug sensitive to myocardial ischemia Mark. Compared with the traditional markers of myocardial injury, the blood concentration of ischemia-modified albumin (IMA) will increase in a short period of time after the onset of ischemia, but at this time the irreversible damage of my...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N21/31
CPCG01N21/78G01N33/6803G01N33/683G01N2021/775
Inventor 孙涛李志明谭柏清甘宜梧王绮李静
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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