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Detection kit of ischemia modified albumins

A technology for detecting kits and albumin, which is applied in the field of clinical testing, can solve problems affecting instrument analysis performance, inconvenient operation, instrument pollution, etc., and achieve the effects of easy promotion and use, strong anti-interference ability, and good stability

Active Publication Date: 2016-03-30
BEIJING STRONG BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This contaminates the instrument, which in turn affects the analytical performance of the entire instrument
[0014] In addition, there are determination methods such as ELISA and HPLC / MS, but due to inconvenient operation, they are rarely used.

Method used

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  • Detection kit of ischemia modified albumins
  • Detection kit of ischemia modified albumins
  • Detection kit of ischemia modified albumins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: the preparation method of kit of the present invention

[0052] According to the following components, the kit is prepared in the form of liquid dual reagents.

[0053] Kit 1. the first of the kit of the present invention consists of a double reagent kit, and its composition is as follows:

[0054] Reagent 1 composition and concentration:

[0055] Good's buffer: 50mM, pH8.0

[0056] Cobalt chloride: 0.3mM,

[0057] TritonX-405: 0.1%;

[0058] Reagent 2 composition and concentration:

[0059]

[0060] Kit 2. The 2nd composition of kit of the present invention is double reagent kit, and its composition is as follows:

[0061] Reagent 1 composition and concentration:

[0062]

[0063] Reagent 2 composition and concentration:

[0064] Good's buffer: 100mM,

[0065] Dithiothreitol: 5mM,

[0066] Tris(2-carboxyethyl)phosphine-hydrochloride: 5mM.

Embodiment 2

[0067] Embodiment 2. The usage method of kit

[0068] The detection method is the rate method.

[0069] 1) provide serum samples,

[0070] 2) After mixing 150 μL reagent 1 and 20 μL serum sample, incubate at 37°C for 5 minutes, and measure the absorbance A1 at 505 nm,

[0071] 3) Add 50 μL of reagent 2, incubate for another 5 minutes, and detect the absorbance A2 at 505 nm for 5 minutes. The concentration of IMA in the sample was thus calculated. Other suitable assays and procedures known in the art may also be used.

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Abstract

The invention relates to a detection kit of ischemia modified albumins. The kit concretely comprises cobalt ions and dithiothreitol. A stabilizer is added to the kit, so the storage stability of the kit is increased; and a solubilizer is also added, so pollution of reagents to a colorimetric device is removed.

Description

technical field [0001] The invention relates to the field of clinical testing, in particular to an improved detection kit for ischemic modified albumin. Background technique [0002] Ischemia-modified albumin (Ischemia-modified albumin, IMA) refers to the albumin whose N-terminal amino acid is modified (the binding site is occupied by copper ions or acetylated or deleted) when tissue ischemia and reperfusion occur. It is the decrease in the ability to combine with transition metals (nickel, copper, cobalt). [0003] IMA rises rapidly within 5-10 minutes after myocardial ischemia and returns to baseline within 6-12 hours, which is an early indicator before cell necrosis after myocardial ischemia. Compared with other indicators that reflect myocardial infarction, such as CK-MB, Myo, and cTn, its kinetic curve shows that the time zone of its generation is more advanced, and the sensitivity of judging myocardial ischemia is higher. [0004] Clinically, IMA is mainly used in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/31G01N21/78
Inventor 宋占科杜爱铭刘瑶刘希
Owner BEIJING STRONG BIOTECH INC
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