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Reagent kit for detecting mouse ischemia modified albumin (IMA) by red light and detection method thereof

A detection method and kit technology, applied in the field of red light detection of ischemia-modified albumin, can solve the problems of unavoidable serious protein interference, limited promotion and use, and affecting sensitivity and specificity

Inactive Publication Date: 2010-02-24
贺坚慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the detection of IMA by the American Bar-Or D method at a wavelength of 450-500nm cannot avoid the serious interference of proteins, especially albumin, lipemia, hemoglobin and bilirubin, which affects the sensitivity and specificity, and limits the promotion and use. Therefore, the development of a A kind of new IMA detection kit and detection method thereof are clinically in urgent need

Method used

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  • Reagent kit for detecting mouse ischemia modified albumin (IMA) by red light and detection method thereof
  • Reagent kit for detecting mouse ischemia modified albumin (IMA) by red light and detection method thereof
  • Reagent kit for detecting mouse ischemia modified albumin (IMA) by red light and detection method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Pyridyl azochromotropic acid-cobalt ion IMA detection kit is used to establish IMA standard curve, calibrator, quality control and detect IMA on Laola FAITH-1020 automatic biochemical analyzer

[0035] The pyridyl azochromic acid-cobalt ion IMA kit consists of the first reagent cobalt ion solution (CoCl 2 ·6H 2 (23.5mg / 100ml) and the second reagent are composed of pyridiniazochromic acid (0.12mg / ml) solution prepared by pH 10 ammonia water-ammonium chloride buffer solution (25 μl / ml ammonia water, 1.2mg / ml ammonium chloride).

[0036] 40 samples of myocardial ischemia were selected from emergency patients with acute chest pain who were cTn I positive or ECG ST wave fluctuations, and 100 samples of the control group were selected from patients over 35 years old, without diabetes, hypertension, hyperlipidemia, and hypercholesterolemia. Blood is drawn into procoagulant tubes, and after ten minutes, clotted blood is centrifuged to separate serum. Then put the sa...

Embodiment 2

[0052] Example 2 Application of the p-nitrodiazoaminoquinolyl azoquinoline-nickel ion IMA kit in the diagnosis of ACS and the risk classification of ACS

[0053] The p-nitrodiazoaminoquinolyl azoquinoline-nickel ion IMA kit consists of a divalent nickel ion solution (NiCl 2 ·6H 2 O, 55.0mg / 100ml) a small test tube with a cover (also a colorimetric tube) and containing 1,000μl of the second reagent consisting of pH9.0 ammonia water-ammonium chloride buffer solution (20μl / ml ammonia water, 7.0mg / ml ammonium chloride) The prepared p-nitrodiazoaminoquinoline azoquinoline (0.10 mg / ml) solution consists of a small test tube with a cover, which is used for whole blood testing.

[0054] The patient's blood is pumped into the anticoagulation test tube containing sodium heparin, the cover of the first reagent is opened, 80 μl of the whole blood to be tested is added to the first reagent tube and mixed, incubated at room temperature for five minutes, and then the cover of the second rea...

Embodiment 3

[0055] Example 3 1,5-(2-hydroxy-5-bromo)-3-cyanomethyl - Zinc ion IMA kit combined with troponin I for ACS screening in high-risk chest pain patients

[0056] 1,5-(2-hydroxy-5-bromo)-3-cyanomethyl - Zinc ion IMA kit consists of the first reagent zinc ion solution (ZnSO 4 ·7H 2 (24.8mg / 100ml) and the second reagent are 1,5-(2-hydroxyl-5- Bromo)-3-cyanomethyl (0.125mg / ml) solution composition.

[0057] Blood from high-risk patients with chest pain was drawn into procoagulant tubes, and after ten minutes the clotted blood was centrifuged to separate the serum. Add 100 μl of the tested serum to a microcuvette, then add 100 μl of the first reagent and mix well, incubate at room temperature for five minutes, then add 1,000 μl of the second reagent and mix well, incubate at room temperature for ten minutes, use a colorimeter, and adjust to zero with air Measure the OD value of the sample (optical path 1 cm, wavelength 660 nm). The obtained OD value is compared with the stan...

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Abstract

The invention discloses a reagent kit for detecting mouse ischemia modified albumin (IMA) by red light and a detection method thereof. The reagent kit consists of a divalent metal ion cobalt, zinc ornickel solution as a first reagent and a metal ion color development reagent with the pH value of 8-10 as a second reagent, or an IMA test paper tape which is subject to color development by the second reagent. The detection method is as follows: mixing a biological sample and a first reagent solution to form a mixed liquid, then adding the second reagent for developing color or dripping the mixedliquid on the IMA test paper tape for developing color to form a blue or green coordination compound, and measuring the absorbance or the reflectivity of the blue or green coordination compound underthe condition that the wavelength of red light is 640-700 nm, wherein the measured value can be used for diagnosing whether a patient has a ischemia symptom or not. The invention develops a method for rapidly detecting IMA, which is suitable for clinically diagnosing, has simple steps, finishes the diagnosis within only about 15min and avoids the influence of interferents in the sample so that the measured result is closer to the truth value. The reagent kit has low cost, high sensitivity, strong specificity, stable performance and convenient transportation and storage.

Description

technical field [0001] The invention relates to a kit for detecting ischemic modified albumin (IMA) with red light and a detection method thereof. This kit and its detection method are mainly to mix the patient's biological sample with the known excess divalent metal ion solution of the first reagent, the mixed solution contains metal ions bound to albumin and not bound to albumin, and then Add the second reagent to the metal ion chromogenic agent solution with a pH of 8-10 to develop color or use IMA test strips to develop color, and detect the metal ion chromogenic agent and cobalt, zinc, and copper not combined with albumin at a red light wavelength of 640-700nm The absorbance or reflectance of the blue complex formed by ions or the green complex formed by nickel ions can be used to diagnose whether a patient is stroke-like. Background technique [0002] Ischemia is usually due to local vascular stenosis or blockage, which leads to a decrease in the oxygen supply capacit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/52
Inventor 贺坚慧程振球刘佳潘曙明江静
Owner 贺坚慧
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