34 results about "Halotolerant bacteria" patented technology

The invention discloses a biochemical treatment process for high salinity wastewater. In order to solve the problem of treating high salinity wastewater with relatively low operating cost and total investment, the biochemical treatment process for high salinity wastewater comprises the following steps: 1) obtaining halotolerant bacteria and attaching the halotolerant bacteria on filler in a reactor; 2) preparing simulated wastewater; 3) acclimatizing the halotolerant bacteria, wherein the simulated wastewater is mixed with the high salinity wastewater in different proportions to prepare various acclimatizing wastewater, and in the starting process of the reactor, the halotolerant bacteria are acclimatized by virtue of the various acclimatizing wastewater according to a sequence of the content of the simulated wastewater in the acclimatizing wastewater from high to low, so that the starting time of the reactor is shortened; 4) directly treating the high salinity wastewater by virtue of the acclimatized halotolerant bacteria. By virtue of acclimation and culture combined with a biological multiplying technology, the halotolerant bacteria are maximized in quantity, specialized in flora and efficient in degrading capability, so that organic pollutants in water are effectively degraded.

The invention discloses a composite microbial agent, a preparation method thereof and a salty sewage treatment method. The composite microbial agent comprises halophilic archaea, halophilic bacteria and halotolerant bacteria. The preparation method comprises the following steps of performing liquid culturing on each component under different salinities respectively, and mixing bacterium solutions to obtain the composite microbial agent when each bacterium solution contains a certain amount of bacteria. The composite microbial agent can be added into salty sewage to treat the salty sewage. Different strains in the composite microbial agent are in different growth states under different salty conditions, and advantages in the growth of the strains within high, medium and low major salty ranges are achieved, so that the microbial agent can be used for treating organic sewage with different salinities, and is wide in application range.

The invention provides a method for brewing soybean sauce by adding halotolerant bacteria to reduce the content of free tyrosine in sauce mash, and in particular relates to a method for screening andseparating halotolerant bacteria consuming tyrosine. The method is characterized by comprising the following steps: (1) detecting the tyrosine content of sauce mash in different high-salt dilute fermentation tanks, selecting sauce mash of which the content of free tyrosine is most obviously reduced than the content of tyrosine in the initial fermentation stage, separating out a strain from the sauce mash, and culturing the strain by using a high-salt culture medium to obtain a halotolerant bacterium original strain; (2) observing the colony form and the thallus microscopic form of the halotolerant bacteria; (3) performing expanding culture on the halotolerant bacteria original strain to obtain a halotolerant bacterium solution; and (4) inoculating the halotolerant bacterium solution into sauce mash containing high free tyrosine for constant-temperature fermentation, and screening out sauce mash with the free tyrosine content less than 100mg / 100g, wherein halotolerant bacteria corresponding to the used bacterium solution are halotolerant bacteria capable of consuming tyrosine. According to the invention, the tyrosine-consuming halotolerant bacteria are separated out for the soybeanpaste brewing process, so that the amount of tyrosine in the later fermentation stage can be reduced, and the risk of white spots generated in the soybean paste can be reduced.

The invention discloses a method for treating pickle wastewater. The method comprises the following steps: (1) cultivating and domesticating special halotolerant bacteria; (2) feeding the pickle wastewater into a grid well to primarily eliminate suspended solids; (3) conveying the wastewater inside the grid well into a conditioning pond for physical sedimentation, and stirring so as to ensure that the water quality is balanced; (4) feeding the wastewater into an anaerobic system to be treated so as to degrade and decompose organisms; (5) feeding the wastewater into an aerobiotic biochemical system for secondary aerobic anoxic treatment, further decomposing the residual organisms by using aerobic halotolerant bacteria and anoxic halotolerant bacteria respectively, and balancing the water quality; (6) performing solid-liquid separation in a secondary sedimentation tank to settle down sludge, and performing filtering treatment in a carbon filtering tank so as to ensure that the effluent is clear in quality. The method is compact in arrangement, the land is saved, impurities such as suspended solids and organisms in the pickle wastewater are sequentially eliminated, the effect of final sewage treatment is greatly improved, and a great deal of expense for extra treatment is saved for companies.

The invention discloses a culture method of halotolerant bacteria capable of removing nitrate in a high-salinity solution, which comprises the following steps: 1) adding a sodium chloride solution into a stirring tank filled with a waste liquid, stirring and carrying out trace aeration, and controlling the concentration of sodium chloride to be 25-35g / L; and 2) continuously adding nitrified sludgeinto the stirring tank, adding glucose to control the COD content to be 1800-2200mg / L, culturing for 2-4 days, adding potassium nitrate, controlling the NO3<-> concentration to be 400-600mg / L, reacting for 60-84 hours, emptying a water sample, and retaining activated sludge, thereby obtaining the halotolerant bacteria. According to the method, the high-salt-tolerance microorganisms can be cultured, and the nitrate removal effect is good. According to the culture method of the halotolerant bacteria, the high-salt-tolerance microorganisms can be cultured, the culture time is short, the cost islow, the occupied area is small, the living conditions of the microorganisms are wide, temperature and pH changes (except for rapid changes) can be borne, and the effect of removing nitrate in the high-salinity solution is good.

The invention discloses a low-density water-retention insect-resistant saline-alkali soil conditioner and a preparation method thereof. The density of a low-density organic bacterial fertilizer is 0.7-1.0 g / cm < 3 >; the organic fertilizer is prepared from 0.5%-1% of medium and trace nutrient elements, 2%-5% of fly ash, 1%-1.8% of ammonium sulfate, 0.1%-0.15% of ferrous sulfate, 0.1%-0.25% of diaminoferric xanthohumate, 1%-1.5% of immobilized beauveria bassiana culture, 1%-1.5% of immobilized metarhizium anisopliae culture, 0.2%-0.5% of a chelating agent, 0.05%-0.1% of a nitrification inhibitor, 0.15%-0.35% of aluminum sulfate and the balance fermented organic matter. The preparation method comprises the steps of mushroom residue treatment, high-temperature fermentation, halotolerant bacteria fermentation, material mixing and the like. The low-density water-retaining insect-resistant saline-alkali soil conditioner has multiple effects of retaining water, inhibiting plant diseases and insect pests, improving the granular structure of saline-alkali soil and the like.

The invention discloses a recycling treatment system of high-salinity high-organic-matter chemical wastewater. The recycling treatment system comprises an electrocatalytic oxidation device, a halotolerant bacteria biochemical system, an MBR aerobic device, an electroosmosis unit, an ultrafilter and an RO unit, the halotolerant bacteria biochemical system comprises a halotolerant bacteria anaerobic device, a halotolerant bacteria aerobic device and a halotolerant biological aerated filter. The invention further discloses a treatment method of the high-salinity high-organic-matter chemical wastewater. By the recycling treatment system and the treatment method, the problem of recycling treatment of the high-salinity high-organic-matter chemical wastewater is solved effectively, recycling rate of water and salt is high, no wastewater is discharged basically, no solid waste is generated, generated water is high in quality, generated salt is high in purity, and water resources and salt in the high-salinity high-organic-matter chemical wastewater are recycled to greatest extent; zero discharging of wastewater in the process of enterprise production is realized, and the recycling treatment system and the treatment method have important environment benefit.

The invention relates to a method for solidifying sand by utilizing halotolerant bacteria. The method selects Sporosarcina pasteurii as bacteria, the strain comes from American Type Culture Collection (ATCC), and the collection number is ATCC 11859. The method comprises the following steps: putting the strain in a nutrient broth medium to obtain a bacterial solution after domestication and enrichment; putting the bacterial solution in desert underground brine containing urea, calcium ions and salinity, and culturing to obtain a bacterial solution with an induced solidification effect; spraying the bacterial solution on the surface of sand soil; putting the sand soil of which the surface is sprayed with the bacterial solution in the natural drying environment for culturing; and naturally air-drying, so that the bacteria can utilize the desert underground brine to cement and solidify sand grains on the surface of the sand soil through the solidification effect so as to prevent the sand soil on the surface layer from migrating under the wind effect. The method is environment-friendly, is low in cost, and can be widely used for solidifying mobile and semi-mobile dunes.

The invention relates to the field of sewage treatment, in particular to a preparation method of a salt-tolerant biological agent and an adding method thereof. The salt-tolerant biological agent comprises a salt-tolerant efficient inoculant and a salt-tolerant efficient bacteria filler, the salt-tolerant efficient inoculant is prepared by mixing and compounding halotolerant bacteria O1 and halotolerant bacteria Y5, according to the salt-tolerant efficient bacteria filler, the salt-tolerant efficient inoculant is immobilized, and loaded on a polyurethane filler. The influence of the composite efficient halotolerant bacteria in different proportions on the removal effect of organic pollutants in sewage is recorded through experiments, so that the optimal configuration proportion of the O1 efficient halotolerant bacteria and the Y5 efficient halotolerant bacteria is explored, and the treatment effect is greatly improved no matter whether the salt-tolerant efficient composite inoculant orthe salt-tolerant efficient composite microbial filler is added; and the salt-tolerant high-efficiency composite bacteria filler has an obvious effect of maintaining TOC degradation efficiency.

The invention discloses an acclimation and culture device for halotolerant bacteria in salt-containing wastewater and a using method of the acclimation and culture device. The device comprises an acclimation zone and a culture zone which are connected through a pipeline, wherein the acclimation zone comprises an anaerobic / anoxic zone, an aerobic zone and an anoxic zone which are communicated in sequence; the culture zone comprises an anaerobic culture zone, an aerobic culture zone and an anoxic culture zone which are not communicated with one another; the aerobic zone is internally provided with a first treatment zone and a second treatment zone which are connected in parallel; the initial end of the anaerobic / anoxic zone is connected with a water inlet pipe, and the anaerobic / anoxic zone, the aerobic zone and the anoxic zone are connected with drain pipes; a first slow-release filler is arranged in each of the first treatment zone and the second treatment zone; and a second slow-release filler is arranged in the anoxic zone. The device is not provided with a sedimentation tank, a sludge backflow device or a stirring device, and is simple in structure, low in equipment cost and operation electric charge, not prone to being damaged and easy to operate.

The invention discloses a method for rapidly removing organic pollutants in coal gas wastewater. The method basically comprises the following steps: (1) selecting strains or mixed flora of halophilic / halotolerant bacteria capable of generating compatible solutes to carry out osmosis-resistant culture under a high-salt environment; (2) centrifugally collecting thalli, putting the thalli into the coal gas wastewater, and carrying out standing until floccules in the wastewater are completely settled to finish the removal of the organic pollutants. According to the method, the pollutants are rapidly separated and removed due to an adsorption effect between the compatible solutes extracellularly released by the thalli and molecules of the organic pollutants (mainly comprising a polycyclic aromatic compound, a heterocyclic compound and petroleum hydrocarbon) and by virtue of the rapid flocculation and sedimentation of the thalli serving as particle frameworks, and the method has the advantages of high speed, high efficiency and no pollution.

The invention discloses a method for treating pickle wastewater. The method comprises the following steps: (1) cultivating and domesticating special halotolerant bacteria; (2) feeding the pickle wastewater into a grid well to primarily eliminate suspended solids; (3) conveying the wastewater inside the grid well into a conditioning pond for physical sedimentation, and stirring so as to ensure that the water quality is balanced; (4) feeding the wastewater into an anaerobic system to be treated so as to degrade and decompose organisms; (5) feeding the wastewater into an aerobiotic biochemical system for secondary aerobic anoxic treatment, further decomposing the residual organisms by using aerobic halotolerant bacteria and anoxic halotolerant bacteria respectively, and balancing the water quality; (6) performing solid-liquid separation in a secondary sedimentation tank to settle down sludge, and performing filtering treatment in a carbon filtering tank so as to ensure that the effluent is clear in quality. The method is compact in arrangement, the land is saved, impurities such as suspended solids and organisms in the pickle wastewater are sequentially eliminated, the effect of final sewage treatment is greatly improved, and a great deal of expense for extra treatment is saved for companies.