41 results about "Genetic screening method" patented technology

The invention relates to a kit for screening dilated cardiomyopathy. The detection genes of the kit are MYBPC3, SCN5A, MYH7 and MYPN. The present invention also provides the application of four genes MYBPC3, SCN5A, MYH7 and MYPN in preparing a kit for screening dilated cardiomyopathy. The advantages of the present invention are: the present invention screens out the most susceptible 4 sporadic dilated cardiomyopathy pathogenic genes in Chinese Han population, and develops an effective early diagnosis method and diagnostic kit for sporadic dilated cardiomyopathy in Chinese Han population, Effectively improve the early detection rate of patients with dilated cardiomyopathy, so as to achieve early treatment and improve the survival rate of patients; compared with the current expensive and inefficient genetic screening methods for dilated cardiomyopathy, the discovery of these four high-risk disease-causing genes It will make screening more targeted, thereby reducing testing costs and improving detection efficiency.

An infectious etiologic agent detection probe set which detects an infectious etiologic agent gene, includes a plurality of kinds of probes including oligonucleotide having base sequences selected from each of a plurality of groups selected from a first group including base sequences of SEQ ID Nos. 1 to 14 and complementary sequences thereof, a second group including base sequences of SEQ ID Nos. 15 to 24 and complementary sequences thereof, a third group including base sequences of SEQ ID Nos. 25 to 36 and complementary sequences thereof, a fourth group including base sequences of SEQ ID Nos. 37 to 47 and complementary sequences thereof, a fifth group including base sequences of SEQ ID Nos. 48 to 57 and complementary sequences thereof, a sixth group including base sequences of SEQ ID Nos. 58 to 68 and complementary sequences thereof, a seventh group including base sequences of SEQ ID Nos. 69 to 77 and complementary sequences thereof, an eighth group including base sequences of SEQ ID Nos. 78 to 85 and complementary sequences thereof, a ninth group including base sequences of SEQ ID Nos. 86 to 97 and complementary sequences thereof, and a 10th group including base sequences of SEQ ID Nos. 98 to 106 and complementary sequences thereof.

The invention discloses a high-throughput functional gene screening method and system for quantitative analysis of cell phenotype images, and belongs to the technical field of gene screening. The images of cells with phenotypes to be screened and those without phenotypes to be screened are captured by a fully automatic fluorescence microscope; converted into black and white binary images of phenotypes to be screened and without phenotypes to be screened respectively; and then divided into individual phenotypes to be screened The image of the cell and the image containing a single cell without a phenotype to be screened; the corresponding part of the image containing a single cell with a phenotype to be screened in the image of a cell with a phenotype to be screened is used as a positive training set, which will contain a single cell without a phenotype to be screened The corresponding part of the cell image in the cell image without the phenotype to be screened is used as a negative training set, and the final model that can identify the cell phenotype is obtained; the image of the gene knockout or overexpression cell to be screened is identified by the final model, and the sample to be screened is obtained. Screening for association of genes with phenotypes. The method improves screening efficiency and accuracy.

Canavan disease, an autosomal recessive leukodystrophy, is caused by deficiency of aspartoacylase and accumulation of N-acetylaspartic acid in brain. Human aspartoacylase (ASP) cDNA spanning 1,435 bp has been cloned and expressed in E. coli. A base change, a854>c, has been found in 85% of the 34 Canavan alleles tested so far, which results in a missense glu285>ala mutation that is predicted to be part of the catalytic domain of aspartoacylase. The invention therefore provides nucleic acid sequences, genes, polypeptides, antibodies, vectors containing the gene, host cells transformed with vectors containing the gene, animal models for the disease, methods for expressing the polypeptide, genetic screening methods and kits, diagnostic methods and kits, methods of treating Canavan disease and methods of genetic therapy for the disease.

The invention discloses a gene rapid screening method capable of simultaneously detecting contaminating bacteria in fruit juice drinks. The method includes the cultivation of juice beverage contaminating bacteria and DNA template extraction, and then through the combination of high-throughput multiplex PCR technology and high-resolution capillary electrophoresis separation technology, it is possible to detect whether the sample contains of contaminating bacteria. Compared with other current molecular biology detection methods based on PCR technology, the present invention can integrate and detect 4 kinds of fruit juice drink contaminating bacteria in one reaction, saving detection cost and detection time, and the detection time is reduced from 7 to 14 days shortened to 8h.

The invention discloses a rapid gene screening method for simultaneously identifying multiple heat-resistant bacteria in fruit juice drinks. The method uses a primer system consisting of 4 pairs of primers, and combines high-throughput multiplex PCR technology with high-resolution capillary electrophoresis separation technology to detect the presence of heat-resistant bacteria in fruit juice beverages. . Compared with other current molecular biology detection methods based on PCR technology, the present invention can integrate and detect 4 kinds of fruit juice beverage heat-resistant bacteria, cycloaliphatic bacillus, cladosporium, spoilage mold, and silk mold in one reaction. Flowers, saving the detection cost and detection time, the detection time was shortened from 7 to 14 days to 8 hours.