Goat F17 escherichia coli infection resisting gene screening method

A technology of Escherichia coli and screening method, applied in the directions of genomics, biochemical equipment and methods, and microbial determination/inspection, etc. The effect of increasing the attack success rate

Pending Publication Date: 2019-05-24
YANGZHOU UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0004] The main purpose of the present invention is to provide a screening method for genes resistant to sheep F17 Escherichia coli infection, aiming to solve the shortcomings of blindness, low efficiency and poor pertinence in traditional gene screening techniques

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  • Goat F17 escherichia coli infection resisting gene screening method
  • Goat F17 escherichia coli infection resisting gene screening method

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Embodiment Construction

[0021] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not indicated in the examples, it is carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased from the market.

[0022] The present invention provides a screening method for a gene resistant to sheep F17 Escherichia coli infection, and the screening method for a gene resistant to sheep F17 Escherichia coli infection includes the following steps:

[0023] Step S1, selecting diseased lambs and disease-resistant lambs infected with Escherichia coli, correspondingly collecting spleen and intestinal content samples of diseased lambs and disease-resistant lambs...

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Abstract

The invention discloses a goat F17 escherichia coli infection resisting gene screening method which includes the steps: F17 escherichia coli infection diseased lamb and disease-resistant lamb screening and spleen and intestinal canal content sample acquisition; F17 escherichia coli infection diseased lamb and disease-resistant lamb spleen sample high-throughput sequencing; F17 escherichia coli infection diseased lamb and disease-resistant lamb spleen transcriptomics comparative analysis; F17 escherichia coli infection resisting gene (circRNA) screening and identifying. The goat F17 escherichiacoli infection resisting gene screening method is used for screening F17 escherichia coli infection resisting genes (circRNA) from disease-resistant lamb spleens in a targeted manner, is high in accuracy and pertinence and saves time.

Description

technical field [0001] The invention relates to the technical field of animal husbandry and veterinary medicine, in particular to a genetic screening method against Escherichia coli infection of sheep F17. Background technique [0002] In the modern intensive sheep production process, the raising of lambs has become one of the most important links. Sheep colibacillosis is one of the most common and high-incidence bacterial diseases in large-scale sheep farms. Traditional antibiotic treatment programs have many defects. In addition, drug residues also seriously affect the quality of animal products. Therefore, it is necessary to find and explore high-quality and safe alternatives to antibiotics. Sheep colibacillosis is an acute infectious disease characterized by severe diarrhea and sepsis caused by Escherichia coli, especially the diarrhea of ​​newborn lambs caused by enterotoxogenic Escherichia coli (ETEC) infection, also known as pullorum of lambs. The disease is mainly ...

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Application Information

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IPC IPC(8): C12Q1/6809C12Q1/6888G16B30/00G16B20/00
Inventor 孙伟鲍建军金澄艳吕晓阳高雯于嘉瑞陈炜昊
Owner YANGZHOU UNIV
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