33 results about "Gene Circuits" patented technology

The invention discloses a method for carrying out gene editing and expression regulation by utilizing a Cas splitting system. A protein group is provided for splitting a Cas9 protein amino acid sequence from different sites to form two-section proteins which form a protein group. Proved by an experiment, an Intein-mediated Ca9 splitting system can efficiently realize gene editing and transcriptional activation regulation on a gene circuit. According to the method disclosed by the invention, the CaS9 splitting system is combined with a tumor cell specific promoter, so that specific detection can be carried out on bladder cancer cells.

Described are methods and compositions for the detection of target genes. The inventors have developed a synthetic nucleic acid sensor-effector gene circuit. In cells without a target gene, the circuit suppresses e.g., effector production, but in the presence of the target gene the suppression is subject to competition, such that the synthetic sensor is de-repressed and permits expression of the effector gene. The methods and compositions described further permit the selective expression of an effector gene in those cells expressing the target gene. In this manner, cells expressing a target gene can be selectively targeted for treatment or elimination. In certain aspects, the methods and compositions described permit the selective expression of an agent such as a therapeutic gene product, in a specifically targeted population of cells in an organism.

Provided are: a selection method for a gene switch and a gene circuit, including using, as a selector, an expression vector containing at least a gene sequence whose expression is controlled by a transcription regulatory factor to be expressed when a genetic switch and a genetic circuit including the genetic switch operate, and a promoter sequence operably linked to the gene sequence upstream thereof; and an expression vector to be used in the selection method. This enables an effective selection method for a genetic switch and a genetic circuit, the selection method being able to be conducted within a short time period and with high selection efficiency and less leakiness.

A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million / day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology.

The invention discloses a method for gene editing and expression regulation by using a split Cas system. The present invention provides a proteome, which splits the amino acid sequence of the Cas9 protein from different positions to form a proteome composed of two pieces of protein; the experiment of the present invention proves that the split Ca9 system mediated by Intein can efficiently realize gene editing Regulation of transcriptional activation with gene circuits. The invention provides that the specific detection of bladder cancer cells can be performed by using the split Cas9 system combined with the tumor cell-specific promoter.

The invention discloses a blue-light-controlled dynamic regulation system and its application, specifically discloses a blue-light-controlled dynamic regulation system and its application in the production of acetoin, belonging to the technical field of bioengineering. The present invention selects the blue light photosensitive protein by means of molecular biology, utilizes the light-sensitive protein’s specific response to the light source characteristic of a specific wavelength and the advantage of not needing to add an inducer from an external source, and introduces the dynamic regulation of targeting FtsZ+FtsA into engineering Escherichia coli The gene circuit realizes the production of acetoin in the inorganic salt medium. The yield of acetoin produced by the invention can reach 63g / L, and the conversion rate can reach 0.57g / g glucose.

The invention discloses a far-red light gene circuit expression control system. The far-red light gene circuit expression control system comprises a photoreceptor for sensing a far-red-light light source, a processing device for processing a signal transmitted by the photoreceptor and an effector for responding the signal transmitted by the processing device. The invention further discloses a gene circuit remote regulation and control system; the gene circuit remote regulation and control system comprises the far-red light gene circuit expression control system, a control device for sending out a remote control instruction and a far-red-light light source device. The invention further discloses application of the far-red light gene circuit expression control system and the gene circuit remote regulation and control system to treatment of diabetes mellitus. The invention further discloses a eukaryotic expression vector, an engineered cell or an engineered cell transplanting vector, which contains the far-red light gene circuit expression control system. The far-red light gene circuit expression control system can be used for rapidly regulating and controlling gene expression and has the characteristics of ultra-remote control, intelligent regulation and control of gene expression quantity, high regulating and controlling expression times, high time-space specificity, strong tissue penetrating power, good insulating property, no toxic or side effect and the like.