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Gene circuit based on lung-cancer marker detection

A marker and gene technology, applied in the field of synthetic biology, can solve the problems of building a gene circuit system and the difficulty of parameter optimization, and achieve the effect of strong anti-interference ability, overcoming interference and stable output.

Active Publication Date: 2014-08-27
BEIHANG UNIV
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Problems solved by technology

However, due to the complexity and difficulty of gene circuit design, the construction, joint debugging and parameter optimization of the gene circuit system are relatively difficult to achieve

Method used

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  • Gene circuit based on lung-cancer marker detection
  • Gene circuit based on lung-cancer marker detection
  • Gene circuit based on lung-cancer marker detection

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Embodiment Construction

[0021] See figure 1 , figure 2 , image 3 , Figure 4 , Figure 5 , Image 6 , Figure 7 As shown, a gene circuit based on lung cancer marker detection in the present invention is implemented as follows: it consists of a marker single-index detection module and a fusion circuit module, and the two modules are sequentially connected to each other. The single index detection module of the detection object detects three lung cancer markers (CEA, CA153, NSE) respectively, and when the concentration of the detected marker is higher than the threshold level, the circuit module can output a pulse signal to the next level fusion circuit. The fusion circuit module is formed by sequentially connecting a three-port input inverter ABC-G and an inverter G-GFP, and the function of the fusion circuit module is to send a pulse signal at any one of the above marker single index detection modules At this time, the green fluorescent protein GFP is output for alarm.

[0022] figure 1 It...

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Abstract

A Gene circuit based on lung-cancer marker detection consists of a marker single-index detection module and a fusion circuit module in sequential connection; the marker single-index detection module consists of an upstream branch and a downstream branch; in the upstream branch, a marker starting circuit Input-XY and an inverter Z-Output are in sequential connection; in the downstream branch, a marker starting circuit Input-Y, an inverter Y-W, an inverter W-Z and an inverter Z-Output are in sequential connection; and the fusion circuit module is formed by sequentially connecting an inverter ABC-G with three-port input and an inverter G-GFP. The gene circuit combines the advantages of the two modules, and is capable of realizing rapid response and logic determination, and the detection and alarm effects of the lung cancer marker are realized. The gene circuit has relatively good practical value and wide application prospect in the technical field of synthetic biology.

Description

technical field [0001] The invention relates to a detection gene circuit based on lung cancer markers, which belongs to the technical field of synthetic biology. Background technique [0002] Tumor markers are chemical substances that reflect the presence of tumors. They either do not exist in normal adult tissues, or their content in tumor tissues greatly exceeds that in normal tissues. Their existence or quantitative changes can indicate the nature of tumors, so as to understand the histogenesis, cell differentiation, and cell functions of tumors. Helping tumor diagnosis, classification, prognosis judgment and treatment guidance. Tumor marker testing is a newer test that can catch cancer at an earlier stage. The traditional methods for detecting tumor markers are serum testing, tissue sections, and immunoassays. The cost and experimental requirements are very high, and there are certain risks. [0003] In the past few centuries, the development of molecular biology and ...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 王翔曾梓臻陈瀚舒程珣
Owner BEIHANG UNIV
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