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Far-red light gene circuit expression control system and eukaryotic expression vector thereof

A control system and red light technology, applied in multidisciplinary fields, can solve problems such as the risk of hypoglycemia and the inability to cure diabetes

Inactive Publication Date: 2017-09-19
叶海峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, diabetes has no cure and requires life-long medication
Diabetic patients need to take hypoglycemic drugs orally or inject insulin every day to maintain blood sugar stability, especially if insulin injection cannot achieve a controlled release of insulin, it is very easy to cause the risk of hypoglycemia

Method used

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  • Far-red light gene circuit expression control system and eukaryotic expression vector thereof
  • Far-red light gene circuit expression control system and eukaryotic expression vector thereof
  • Far-red light gene circuit expression control system and eukaryotic expression vector thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0183] (4) Preparation of Escherichia coli Competent Cells

[0184] All solutions and consumables used for the preparation of competent cells are sterilized by high temperature and high pressure.

[0185] 1. Streak Escherichia coli DH5α strain on a plate without antibiotics, and culture it upside down at 37°C for 12-16 hours;

[0186] Pick a single colony and place it in a 2mL LB shaker tube without any antibiotics, shake it at 180rpm at 37°C overnight;

[0187] 2. Take 1mL of the bacterial liquid and transfer it to a 250mL Erlenmeyer flask with LB medium for expansion culture (according to the ratio of 1:100 to expand the culture), shake the culture at 180rpm at 37°C for 2-3 hours until the OD600 is between 0.4-0.5;

[0188] 3. Transfer the culture medium into a centrifuge tube, place it on ice for 10 minutes, then centrifuge at 3000 rpm for 10 minutes at 4°C (the centrifuge needs to be pre-cooled in advance), and carefully discard the supernatant;

[0189] 4. Add pre-coole...

Embodiment 1

[0238] Embodiment 1, the production of an intelligent control system for ultra-remotely controlling a far-red light source by a smart phone through a local area network WiFi or a 2G / 3G / 4G network

[0239] In the present invention, taking the intelligent controller made by the smart home studio and its matching APP, 6 multi-channel output switching power supplies, and 24 far-infrared LED lights as the far-red light source as an example, it is illustrated that the smart phone passes through the local area network WiFi or 2G / 3G / 4G network ultra-remote control of the intelligent control system of the far-red light source, but does not limit the protection scope of the present invention.

[0240] The first step is the implementation of the APP client. The matching APP provided by the purchased smart controller manufacturer (see Experimental Materials and Methods for details), and its specific settings and usage methods are detailed in the manufacturer's instruction manual. For th...

Embodiment 2

[0246] Example 2, construction of remote control system elements for gene loops regulating transgene expression by far-red light

[0247] This example includes the construction method of representative elements in the gene loop remote regulation system for regulating the expression of transgenes by far-red light, but it does not limit the protection scope of the present invention. The detailed design scheme and steps are shown in Table 1.

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Abstract

The invention discloses a far-red light gene circuit expression control system. The far-red light gene circuit expression control system comprises a photoreceptor for sensing a far-red-light light source, a processing device for processing a signal transmitted by the photoreceptor and an effector for responding the signal transmitted by the processing device. The invention further discloses a gene circuit remote regulation and control system; the gene circuit remote regulation and control system comprises the far-red light gene circuit expression control system, a control device for sending out a remote control instruction and a far-red-light light source device. The invention further discloses application of the far-red light gene circuit expression control system and the gene circuit remote regulation and control system to treatment of diabetes mellitus. The invention further discloses a eukaryotic expression vector, an engineered cell or an engineered cell transplanting vector, which contains the far-red light gene circuit expression control system. The far-red light gene circuit expression control system can be used for rapidly regulating and controlling gene expression and has the characteristics of ultra-remote control, intelligent regulation and control of gene expression quantity, high regulating and controlling expression times, high time-space specificity, strong tissue penetrating power, good insulating property, no toxic or side effect and the like.

Description

technical field [0001] The present invention relates to multidisciplinary interdisciplinary fields such as synthetic biology, optogenetics, and electronic engineering, and specifically relates to a gene loop including a far-red light gene loop expression control system, including the far-red light gene loop expression control system Remote control system, control method and application thereof. Background technique [0002] Synthetic biology is a comprehensive discipline that designs and synthesizes various complex biological functional modules, systems and even artificial life forms under the guidance of engineering theory, and is applied to the production of specific chemicals, biomaterials, gene therapy, tissue engineering, etc. . After nearly a decade of rapid development, synthetic biology has made significant progress in many application fields. Especially in the field of mammalian cell synthetic biology, people have designed and constructed a variety of controllable...

Claims

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Application Information

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IPC IPC(8): C12N15/63C12N15/85C12N5/10
CPCC12N15/63C12N15/85C12N2800/107C12N2830/002
Inventor 叶海峰邵佳伟薛帅余贵玲杨雪平于袁欢朱苏承白郁
Owner 叶海峰
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