Establishing method of logic and gate gene circuit for detecting bladder cancer

Abstract

The invention discloses an establishing method of a logic and gate gene circuit for detecting bladder cancer. The method includes the steps of firstly, amplifying an hUPPII promoter sequence shown in SEQ ID NO:1, an aminoacyl tRNA synthetase AcKRS gene sequence shown in SEQ ID NO:2, an hTERT promoter sequence shown in SEQ ID NO:3, a tRNA sequence shown in SEQ ID NO:4 and a pCMV6-AC-GFP37TAG plasmid sequence shown in SEQ ID NO:6 through a PCR method; secondly, connecting an hUPPII promoter, an AcKRS gene, an hTERT promoter and tRNA into a psiCHECK-2 plasmid shown in SEQ ID NO:7 to establish a recombinant vector; thirdly, transplanting the recombinant carrier and a pCMV6-AC-GFP37TAG plasmid into bladder cancer cells to establish the gene circuit. When the gene circuit is transplanted into bladder cancer cells and acetylated lysine is added, green fluorescent proteins are only expressed in bladder cancer cells instead of being expressed in normal cells.

Description

technical field [0001] This application relates to a method for constructing a logical AND gate gene circuit for bladder cancer detection and its application. Background technique [0002] Bladder cancer is the most common malignant tumor of the urinary system and one of the ten most common tumors in the whole body. Due to the lack of highly specific targets for bladder cancer, the current diagnosis and treatment methods cannot effectively distinguish between normal cells and bladder cancer cells through a single target. There is an urgent need to develop more specific and precise treatments for bladder cancer. The study of bladder cancer systems biology and genomics makes it possible for people to have a more comprehensive understanding of tumor cell gene mutations and potential therapeutic targets. Contents of the invention [0003] The invention provides a new kit for detecting bladder cancer. [0004] The present invention provides a method for constructing a logical...

AI Technical Summary

Problems solved by technology

Due to the lack of highly specific targets for bladder cancer, the current diagnosis and treatment methods cannot effectively distinguish between normal cells and bladder cancer cells through a single target. There is an urgent need to develop more specific and precise treatments for bladder cancer.

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