The invention relates to a preparation method of an SRB (
sulfate reducing bacterium) biological inhibitor, which comprises the following steps: by taking
polyvinyl alcohol,
sodium alginate, denitrifying bacterium suspension,
calcium carbonate,
boric acid,
calcium chloride,
silicon dioxide and water as raw materials, preparing an
embedding agent, then preparing a microbial
mixed solution, and finally preparing to obtain immobilized denitrifying bacterium balls. The prepared SRB biological inhibitor can be used in the inhibition of SRB in
crude oil.
Sodium nitrate particles are firstly added into an
oil well casing port; then, the SRB biological inhibitor is added; and finally, water is added to flush the two chemicals to the bottom of a well, and the
dosing interval of each time is 15 days, thus achieving the purpose of inhibiting the growth of SRB for a long time. According to the invention, by using the
sodium nitrate particles to provide a
nitrogen source for the
denitrifying bacteria, an oil layer origin and exogenous denitrifying bacterium microorganisms are activated, and the original and exogenous microorganisms are utilized to change the growth environment of the
system so as to quickly inhibit the growth of SRB in the
oil well, thereby inhibiting the generation of
hydrogen sulfide gas from the source and reducing
corrosion and
sulfide hazard caused by SRB.