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Preparation of SRB (sulfate reducing bacterium) biological inhibitor and method for inhibiting SRB in crude oil

A technology of biological inhibitor and embedding agent, applied in chemical instruments and methods, earthwork drilling, drilling composition, etc., can solve the problem of reducing sterilization efficiency, aggravating corrosion of on-site pipelines and equipment, and killing sulfate-reducing bacteria. In order to achieve the effect of enhancing the bearing capacity, reducing corrosion and sulfide hazards

Active Publication Date: 2013-05-22
SHAANXI URSTAR ENVIRONMENTAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The activity of SRB flora can produce toxic gas H 2 S, to carry H in the oil produced in the formation 2 S, endangers personal safety and increases equipment corrosion. In addition, the secondary product FeS formed by the metabolic activity of sulfate reducing bacteria will also cause non-selective blockage of the formation, further aggravating the corrosion of on-site pipelines and equipment
[0003] At present, among the methods of directly killing sulfate-reducing bacteria physically or chemically, the physical sterilization method has a significant effect on killing free sulfate-reducing bacteria, but due to the limitation of the scope of action, it is not suitable for long-distance sulfate-reducing bacteria or adherent bacteria. Sulfate-reducing bacteria are difficult to achieve the killing effect
In the chemical sterilization method, the widely used fungicides mainly include quaternary ammonium salts, aldehydes, heterocyclics and their complexes, but they are protected because sulfate-reducing bacteria often coexist with other microorganisms in the polysaccharide gum produced by microorganisms. Generally speaking, fungicides are not easy to penetrate; for general oxidizing fungicides, because microorganisms are in a reducing environment of hydrogen sulfide, it is difficult for fungicides to have an effective bactericidal effect; the existence of biofilm reduces the sterilization efficiency, Even ineffective, so that drug-resistant bacteria are produced; and the extensive use of fungicides also brings new pollution loads to the environment
Sulfate-reducing bacteria have a low kill rate, and oilfield equipment is still subject to sulfur ion corrosion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method of SRB biological inhibitor, using polyvinyl alcohol, sodium alginate, Pseudomonas putida bacteria suspension, calcium carbonate, boric acid, calcium chloride, silicon dioxide and water as raw materials, the preparation process is as follows:

[0023] (1) Preparation of embedding agent:

[0024] Add polyvinyl alcohol, sodium alginate, calcium carbonate and water in the reactor, its percentage mass concentration meter polyvinyl alcohol 6%, sodium alginate 0.6%, calcium carbonate 0.5%, the rest is water; Stir and mix well, heat to 80°C and then keep the temperature, stir until completely dissolved, then cool to 35°C and store at constant temperature;

[0025] (2) Preparation of microbial mixture:

[0026] Will contain 8 x 10 9 pcs / ml Pseudomonas putida suspension and the embedding agent prepared in step (1) were mixed according to the volume ratio of 1:2;

[0027] (3) Preparation of immobilized denitrifying bacteria pellets:

[0028] Add the micro...

Embodiment 2

[0030] A preparation method of SRB biological inhibitor, using polyvinyl alcohol, sodium alginate, Pseudomonas putida bacteria suspension, calcium carbonate, boric acid, calcium chloride, silicon dioxide and water as raw materials, the preparation process is as follows:

[0031] (1) Preparation of embedding agent:

[0032] Add polyvinyl alcohol, sodium alginate, calcium carbonate and water in the reactor, its percentage mass concentration meter polyvinyl alcohol 10%, sodium alginate 2%, calcium carbonate 0.3%, the rest is water; Stir and mix well, heat to 80°C and then keep the temperature, stir until completely dissolved, then cool to 35°C and store at constant temperature;

[0033] (2) Preparation of microbial mixture:

[0034] Will contain 8 x 10 9 pcs / ml Pseudomonas putida suspension and the embedding agent prepared in (1) were mixed according to the volume ratio of 1:2;

[0035] (3) Preparation of immobilized denitrifying bacteria pellets:

[0036] Add the microbial m...

Embodiment 3

[0038] A kind of preparation method of SRB bioinhibitor, with polyvinyl alcohol, sodium alginate, Thiobacillus denitrificans bacteria suspension, calcium carbonate, methyldiethanolamine, sodium nitrite, active carbon, boric acid, calcium chloride, silicon dioxide And water is the raw material, and the preparation process is:

[0039] (1) Preparation of embedding agent:

[0040] Add polyvinyl alcohol, sodium alginate, calcium carbonate and water to the reactor, its percentage mass concentration is 8% polyvinyl alcohol, 1% sodium alginate, 0.4% calcium carbonate, and the rest is water; the above materials in the reactor Stir and mix well, heat to 80°C and then keep the temperature, stir until completely dissolved, then cool to 35°C and store at constant temperature;

[0041] (2) Preparation of microbial mixture:

[0042] Will contain 8 x 10 8 pcs / ml Thiobacillus denitrificans suspension and the embedding agent prepared in step (1) were mixed according to the volume ratio of 1...

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Abstract

The invention relates to a preparation method of an SRB (sulfate reducing bacterium) biological inhibitor, which comprises the following steps: by taking polyvinyl alcohol, sodium alginate, denitrifying bacterium suspension, calcium carbonate, boric acid, calcium chloride, silicon dioxide and water as raw materials, preparing an embedding agent, then preparing a microbial mixed solution, and finally preparing to obtain immobilized denitrifying bacterium balls. The prepared SRB biological inhibitor can be used in the inhibition of SRB in crude oil. Sodium nitrate particles are firstly added into an oil well casing port; then, the SRB biological inhibitor is added; and finally, water is added to flush the two chemicals to the bottom of a well, and the dosing interval of each time is 15 days, thus achieving the purpose of inhibiting the growth of SRB for a long time. According to the invention, by using the sodium nitrate particles to provide a nitrogen source for the denitrifying bacteria, an oil layer origin and exogenous denitrifying bacterium microorganisms are activated, and the original and exogenous microorganisms are utilized to change the growth environment of the system so as to quickly inhibit the growth of SRB in the oil well, thereby inhibiting the generation of hydrogen sulfide gas from the source and reducing corrosion and sulfide hazard caused by SRB.

Description

technical field [0001] The invention belongs to the technical field of petroleum gathering and transportation, and relates to a method for inhibiting sulfate-reducing bacteria by using microorganisms, in particular to the preparation of an SRB biological inhibitor and the method for inhibiting SRB in crude oil. Background technique [0002] In the crude oil gathering and transportation system, the most harmful to the surface oil recovery system of the oil field is the sulfate reducing bacteria (SRB for short). The general term for a variety of bacteria, including bacteria of the genus Desultphovibrio and Desulphfotomaculum, widely exist in oilfield sewage reinjection systems and oil reservoir anoxic environments. The activity of SRB flora can produce toxic gas H 2 S, to carry H in the oil produced in the formation 2 S, endangers personal safety and increases equipment corrosion. In addition, the secondary product FeS formed by the metabolic activity of sulfate-reducing bac...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04C09K8/54C09K8/582
Inventor 金鹏康赵楠李岩周立辉任建科
Owner SHAANXI URSTAR ENVIRONMENTAL SCI & TECH
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