44 results about "Bone morphogenetic protein 4" patented technology

The invention provides an inducing agent for inducing directional differentiation of an embryonic stem cell into keratinocytes. The inducing agent comprises bone morphogenetic protein 4, retinoic acid and ascorbic acid. Preferably, in the inducing agent, the bone morphogenetic protein 4, retinoic acid and ascorbic acid are in a content ratio of (1-50g):(0.1-5 mol):(50-600mol). The inducing agent for inducing directional differentiation of the embryonic stem cell into keratinocytes combines the bone morphogenetic protein 4, retinoic acid and ascorbic acid, can effectively improve the efficiency for induced directional differentiation of hESC into keratinocytes (as high as 77.93%, and far higher than other formula inducing agents), provides an approach for efficiently inducing differentiation of hESC into keratinocytes, and provides seed cells for constructing tissue engineered oral mucosas.

The present invention relates to methods for preparing pluripotent cardiovascular precursor cells and maintaining their cardiovascular differentiation ability. For the first time, a method for efficiently inducing the differentiation of pluripotent stem cells into pluripotent cardiovascular precursor cells was revealed, including induction with ascorbic acid, bone morphogenic protein 4 and glycogen synthase kinase 3 inhibitors. The present invention also provides a method for stably cultivating multipotential cardiovascular precursor cells, including using BMP signaling pathway inhibitors, Activin / Nodal signaling pathway inhibitors and glycogen synthase kinase 3 signaling pathway inhibitors to enable stable growth and passage. The multipotential cardiovascular precursor cells obtained by the present invention can be further differentiated into some cells of cardiovascular lineage such as cardiomyocytes, vascular smooth muscle cells or vascular endothelial cells, which can be used for the treatment of heart diseases and research on myocardial regeneration, drug cardiovascular cytotoxicity Testing, cardiac drug development.

The invention relates to a culture medium for sweat gland cells and a preparation method of the culture medium. The culture medium comprises a basic culture solution and an additive, wherein the basic culture solution is an SGDM culture solution; the additive comprises the following components, by the addition amount in the basic culture solution, nerve growth factors, transforming growth factors-beta, epidermal growth factors, HEPG (heparin sulfate proteoglycan) and bone morphogenetic protein 4. The culture medium can increase the probability at which ips cells are induced into sweat gland cells, stable passage can be guaranteed, the cell proliferation speed is increased, and a source of seed cells is provided for skin tissue engineering. Additionally, no serum is used in the whole process, risks caused by animal origin pathogens are effectively avoided, and the clinical application safety is improved.

The invention discloses a culture medium, a kit, a method for preparing early stage mesoderm ancestral cells and a method for preparing hematogenesis endothelial cells by using the kit, wherein the culture medium contains a basic culture medium namely an SFDM (serum-free differentiation medium), PGE2 (prostaglandin E2) and BMP4 (bone morphogenetic protein 4). By using the culture medium disclosed by the invention, a large amount of early stage mesoderm ancestral cells can be prepared efficiently and quickly.