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Pharmaceutical composition containing sodium alginate and preparation method of pharmaceutical composition

A technology of sodium alginate and composition, applied in the field of medicinal compositions containing sodium alginate, can solve the problems of poor healing, patient pain, and difficulty in achieving joint function improvement and recovery, and achieves improved prognosis and improved quality of life. , the effect of improving operability and success rate

Inactive Publication Date: 2015-06-03
PEKING UNIV THIRD HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, relying solely on Mosaicplasty technology cannot obtain a completely flat and continuous articular surface, and it is difficult to achieve better joint function improvement and recovery
And the "donor area" (the bone harvesting area of ​​the autologous osteochondral column) also has poor healing, resulting in pain in the "donor area" of patients after surgery

Method used

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  • Pharmaceutical composition containing sodium alginate and preparation method of pharmaceutical composition
  • Pharmaceutical composition containing sodium alginate and preparation method of pharmaceutical composition
  • Pharmaceutical composition containing sodium alginate and preparation method of pharmaceutical composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] 150ml composition formula:

[0077]

[0078] Preparation:

[0079] (1) Extracting and preparing bone morphogenetic protein-4 transfected adipose-derived stem cells;

[0080] Obtained from fat tissue in the groin of the body. Rinse with a large amount of PBS solution to remove surface blood, cut into pieces with ophthalmic scissors in a 1:1 configuration of PBS and 0.1% type Ⅰ collagenase, digest at 37°C for 30 minutes, neutralize the digestion solution with DMEM containing 10% fetal bovine serum, take Centrifuge at 300g for 5min, discard the suspension containing mature adipocytes, add 160mM NH4Cl erythrocyte lysate to the sediment containing adipose tissue-derived stem cells, let it stand at room temperature for 10min, pass it through a 200-lm nylon sieve, collect the filtrate, Use DMEM medium containing 10% FBS, 1% blue chain double antibody at 37°C / 5% CO 2 incubator overnight. The primary cells were cultured for 4-5 days, and after they grew confluently, they ...

Embodiment 2

[0090] 150ml composition formula:

[0091]

[0092] Preparation:

[0093] The difference from Example 1 is:

[0094] (1) Extract and prepare adipose-derived stem cells transfected with bone morphogenetic protein-4; the primary cells are marked as passage 0, and the cells used are passage 5; ADSCs transfected with BMP-4 with a transfection rate greater than 90% were obtained at about -65 Store under the environment of ℃ (the transfection step is carried out within 48 hours before the application of the composition), and it is ready for use.

[0095] ⑵Preparation of sodium alginate solution: (100ml)

[0096] Weigh 0.6g of HEPES, 0.75g of sodium chloride, dissolve in 70ml of deionized water, heat to 70°C, keep warm, add 2.1g of sodium alginate, keep stirring until the solution is evenly mixed, after cooling to room temperature, add dropwise 1N hydrochloric acid, adjust the pH to 7.35, add deionized water to 100ml, set aside.

[0097] (3) Preparation of calcium chloride sol...

Embodiment 3

[0101] 150ml composition formula:

[0102]

[0103] Preparation:

[0104] The difference from Example 1 is:

[0105] (1) Extract and prepare adipose-derived stem cells transfected with bone morphogenetic protein-4; the primary cells are marked as passage 0, and the cells used are passage 7; ADSCs transfected with BMP-4 with a transfection rate of more than 70% are taken at about -75 Store in ℃ environment, spare

[0106] ⑵Preparation of sodium alginate solution: (100ml)

[0107] Weigh 0.36g of HEPES, 1.05g of sodium chloride, dissolve in 95ml of deionized water, heat to 50°C, keep warm, add 2.7g of sodium alginate, keep stirring until the solution is evenly mixed, after cooling to room temperature, add 1N Adjust the pH to 7.45 with hydrochloric acid, add deionized water to 100ml, and set aside.

[0108] (3) Preparation of calcium chloride solution: 50ml

[0109] Weigh 0.75g of calcium chloride and 0.09g of HEPES, dissolve them in 25ml of deionized water, add dropwise 1...

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Abstract

The invention provides a pharmaceutical composition containing sodium alginate for cartilage tissue repairing. As an active ingredient of the composition, bone morphogenetic protein-4 transfected fat is derived from stem cells, sodium alginate and calcium chloride; by virtue of the composition, gaps filled between cartilage rods as well as between the cartilage rods and peripheral cartilages and subchondral bones are completely filled by regenerated tissues and mechanical properties similar to that of normal cartilage are guaranteed; full fusion of mosaic bones and cartilages with peripheral cartilages and subchondral bones is achieved, and joint function improvement and level recovery in postoperative patients are significantly improved; in addition, the invention also provides a preparation method of the pharmaceutical composition.

Description

technical field [0001] The invention relates to a composition containing sodium alginate and a preparation method thereof, in particular to a medicinal composition containing sodium alginate which can promote bone and cartilage tissue repair. Background technique [0002] Intra-articular osteochondral compound injuries caused by diseases such as trauma and arthritis are very common in clinical practice. Once the cartilage tissue that occupies an important position in the joint is destroyed, it will greatly affect the normal function of the joint. The resulting vicious cycle leads to further disturbance of the internal environment of the joints, abnormal mechanical conduction leads to chain damage of normal articular cartilage, and finally leads to consequences such as restrictions on voluntary movement to varying degrees, which seriously affects the physical and mental health of the majority of patients, and also poses a threat to families and families. society is a heavy bu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/54A61L27/20A61L27/02A61L27/52A61K38/18A61K35/28A61P19/04A61K31/734A61K33/14
Inventor 王健全陈临新敖英芳张辛鞠晓东石媛媛
Owner PEKING UNIV THIRD HOSPITAL
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