The invention belongs to the technical field of food processing, and particularly discloses a red tricholoma matsutake sauce and a preparation method thereof, the red tricholoma matsutake sauce is prepared from the following raw materials by weight: 160-170 parts of red tricholoma matsutake, 20.1-20.9 parts of snake gourd, 15.1-15.9 parts of sword bean, 6.1-6.9 parts of mutton, 4.1-4.9 parts of yellow rice wine, 13.1-13.9 parts of wheat kernel, 7.1-8.9 parts of Chinese toon sprout, 20.1-20.9 parts of black bean, 18-19 parts of cod meat, 24.1-24.9 parts of straw mushroom, 3-10 parts of grapefruit, 3.1-3.9 parts of fresh cordate houttuynia, 4.1-4.9 parts of avocado, and 15.1-15.9 parts of dried bean curd. The red tricholoma matsutake is adopted to replace tricholoma matsutake to serve as a main raw material to prepare the mushroom sauce, the prepared seasoning sauce not only can meet the pursuit of consumers for food taste, but also has certain health-preserving and health-care values, and the red tricholoma matsutake can be artificially cultivated and is high in yield and low in cost, so that the prepared tricholoma matsutake sauce is relatively low in preparation cost, the requirements of consumption personnel in different levels can be met, the application range is wide, and the edible value is high.