The invention discloses a human-computer interaction testing method and system for a mobile terminal, including an eye tracking subsystem, a mobile terminal screen acquisition subsystem, a control center, an eye tracking subsystem, a mobile terminal screen acquisition subsystem and a control center respectively. Central connection, the eye tracking subsystem is used to obtain eye track information that meets the requirements by automatically adjusting the position of the eye tracker, and the mobile terminal screen acquisition subsystem is used to obtain the screen video information of the mobile terminal by exporting real-time screen information of the mobile screen; control The center is used to convert the eye track information into eye track video information, adjust the position of the eye tracker according to the eye track image, perform data collection and coordinate transformation on the eye track video information and mobile terminal screen images, and combine the screen video information with the eye track Video information is superimposed to obtain human-computer interaction test results. This application automatically adjusts and accurately captures the convergence point of eyesight; exports the screen image of the mobile terminal, the data is more accurate, and the test effect is improved.