The invention discloses a device for layered collection and preservation of deep lake water samples, comprising a frame assembly, a sampling and preservation device, a transmission device, a power device and a diversion device, the frame assembly and the sampling and preservation device are tightly connected, and the sampling and preservation device One side of the storage device communicates with one side of the transmission device, the one side of the transmission device is fastened to one side of the frame assembly, the frame assembly is fastened to the power device, the frame assembly includes a frame, and the outer ring of the frame is provided There is a deflector, the frame is tightly connected to the deflector, the power device includes a subsidence component and a jacking component, the bottom side of the subsidence component is tightly connected to the upper end of the frame, and the upper side of the jacking component is tightly connected to the lower end Fixed connection, the body of the cylinder is provided with a fixing groove, the upper end of the cylinder body is provided with a horizontal plate, the horizontal plate is tightly connected with the cylinder body, the lower end of the cylinder body is provided with a bottom plate, and the outer circular surface of the bottom plate is tightly connected to the inner wall of the fixing groove. Fixed connection.