45results about How to "Good survival rate" patented technology

The invention discloses an antibiotic-free conservation fodder for piglets. The conservation fodder comprises the following components in part by weight: 550-640 parts of maize, 40-60 parts of super steam fish meal, 200-250 parts of peeled soybean meal, 40-60 parts of whey powder, 20-30 parts of glucose, 1-2 parts of table salt, 5-9 parts of secondary calcium phosphate, 10-25 parts of Nengbao, 7-12 parts of calcium formate, 10 parts of premix of the conservation fodder for piglets, 6-11 parts of lysine, 1 part of acidifying agent (coated), premix of vitamins, premix of compound minor elements for piglets, phytase, multienzyme, antibacterial peptide, prebiotics, antioxidant and zeolite powder. The antibiotic-free conservation fodder for piglets does not contain any antibiotics, has no defect of drug resistance of animals, drug residues, environmental pollution and the like, and can rival with or even exceed the antibiotic-contained piglet conservation fodder in the prior art in controlling diarrhea and promoting growth of the piglets.

The invention discloses a nanometer sustained-release compound aquaculture feed and a feeding method thereof. The nanometer sustained-release compound aquaculture feed is prepared from the following component in parts by weight: 30-35 parts of high-protein-content whole potato powder. The nanometer sustained-release compound aquaculture feed and the feeding method thereof are complete in protocol and simple in technology. The feeding technology and the rationally matched multiple raw materials in the feed are coordinated, so that the nutrients in the raw materials are mutually complementary; thus, the feed is high in protein content. The chitosan is a kind of natural macromolecular polysaccharide, and has the performance characteristics that the chitosan is wide in source, non-toxic, tasteless, good in biocompatibility, good in biodegradation, and good in antibacterial properties and so on. Thus, the feed is rich and comprehensive in nutrients, good in palatability, low in feed coefficient, and capable of improving immunity and antioxidant capacity of the tinca tinca. Moreover, the tinca tinca fed with the feed is high in survival rate.

The invention relates to a method for manufacturing a seaweed seed bottom laying layer. The method comprises the steps that seaweed seeds are collected and pretreated; the pretreated seaweed seeds and a matrix are uniformly mixed to form a mixture; net cloth is selected as a bottom laying layer net frame and folded to form the double-layer bottom laying layer net frame, and the double-layer bottom laying layer net frame is sewn with twine in an equally spaced mode so that multiple interlayers of the double-layer bottom laying layer net frame can be formed; the interlayers of the net frame are filled with the mixture from the open end of the net frame, the open end is sewn with twine, and then the seaweed seed bottom laying layer is formed; the manufactured seaweed seed bottom laying layer is placed in a water tank, transported to a bottom laying site and directly put into the sea. Materials adopted in the method are all natural materials and can gradually degrade after being planted underwater without causing secondary pollution to the environment. The method facilitates planting, laying, management and maintenance of seaweed.

The invention discloses a micro-nano composite structure surface matched with circulating tumor cell size, a preparation method and application thereof, and belongs to the technical field of the biologics. The preparation method comprises the following steps: preparing a micro-nano composite structure array matched with a to-be-detected tumor cell size on the surface of the substrate, and then fixing specific antibody on the tumor cell surface on the surface of the structure area, utilizing the structure array to synergistically effect with the specific antibody to realize the efficient and specific capturing on the circulating tumor cell. The theory of designing the micron/nanometer structure is designed according to the target cell size can be further used for detecting other cells, thereby providing a new path for the preparation of the biological detection substrate. The substrate prepared through the invention can efficiently and specifically capture the circulating tumor cell, and has the features of being simple in preparation and low in cost; the captured cell has good survival efficiency, and a new path is provided for the preparation of the biological detection substrate.

The invention discloses a tissue culture method for pineapples. The tissue culture method comprises the following steps: (1) sterilizing and disinfecting crown buds of the pineapples, cutting the crown buds up to serve as an explant, putting the explant into a basic culture medium for primary induction, so as to obtain a callus; (2) putting the callus into an induction culture medium for secondaryinduction, so as to hasten the generation of adventitious buds; (3) dicing the callus with the adventitious buds so as to obtain a callus block with at least two adventitious buds, putting the callusblock into a proliferation culture medium for proliferation culture, so as to obtain a proliferated bud block; (4) putting the proliferated bud block into a rooting culture medium for rooting culture, so as to obtain a tissue culture seedling; and (5) hardening the tissue culture seedling. The tissue culture method for the pineapples has excellent survival rate and reproduction rate.

The invention belongs to the technical field of plant seedling reproduction, and discloses a cutting cultivation method for wax begonias. The cutting cultivation method includes selecting one-year-oldhealthy twigs, free from plant diseases and insect pests, with terminal buds, short internodes, strong sprouts and branches, cutting the twigs, pinching off lower leaves, and flattening the lower sides of node orders of bases of the twigs by a blade; soaking the lower portions of cuttings in a composite solution for more than 30 seconds; inserting the cuttings into holes after soaking, and watering the cuttings after compacting; covering the cuttings with plastic bags with holes for moistening, selecting a shading net with the light transmittance of 70% for lighting management, checking rooting conditions, and performing transplant. The cutting cultivation method has the advantages of vigorous growth of root systems, high quantity of roots and high survival rate.

The invention provides a cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions. The cutting seedling culture method of pileostegia viburnoidesunder subtropical high-altitude climatic conditions comprises the following steps of (1) preparing cutting substrates: uniformly mixing peat soil and yellow soil to obtain mixtures to be used as the cutting substrates; (2) preparing cutting panicles; (3) performing soaking with rooting powder: soaking the cutting panicles cut in the step (2) in heteroauxing rooting powder; (4) performing cutting;(5) performing moisture management; and (6) performing illumination management. The cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions disclosed by the invention has the advantages that the pileostegia viburnoides can be well planted at high-altitude low-temperature regions, the cutting seedling breeding speed is high, the cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions is labor-saving, effortless and environmentally-friendly, pileostegia viburnoides seedlings are easy tosurvive after being transplanted, and the cutting seedling culture method of pileostegia viburnoides under subtropical high-altitude climatic conditions has favorable repairing effects on vegetation destruction caused by manual activities of way building, mine exploiting and the like at subtropical mountain massifs.

The invention relates to the technical field of seedling breeding, in particular to a Portuguese oyster triploid artificial seedling breeding technology, which comprises the following steps: hybridizing cultivated crassostrea gigas tetraploid and cultivated Portuguese oyster diploid to obtain Portuguese oyster triploid fertilized eggs, cultivating the Portuguese oyster triploid fertilized eggs to juvenile mollusks, and culturing the juvenile mollusks to obtain the Portuguese oyster triploid artificial seedling. The juvenile mollusks are cultivated into Portuguese oyster triploid adults; proper temperature and salinity are provided for culture of the Portuguese oyster triploid, and the cultured Portuguese oyster triploid has a good survival rate.