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Tissue culture method for succulent plant

A technology for tissue culture and succulent plants, applied in the field of tissue culture of succulent plants, can solve the problems of restricting the cultivation of succulent plants, low survival rate, low reproduction efficiency, etc.

Inactive Publication Date: 2018-05-18
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The commonly used propagation methods of succulents are: grafting, cutting and sowing; no matter which of the above-mentioned propagation methods, there are defects of low survival rate and low reproduction efficiency, which limits the cultivation of succulents.

Method used

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  • Tissue culture method for succulent plant
  • Tissue culture method for succulent plant
  • Tissue culture method for succulent plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) The leaves of the succulent plants (Jade Pearl) are sterilized (the leaves are first soaked in 65% by weight alcohol aqueous solution for 40s, then soaked in 4% by weight sodium hypochlorite aqueous solution for 4min, and finally cleaned by distilled water for 4 minutes) times), then the leaves were minced as explants (0.4 cm in length and 0.2 cm in width), and then the explants were placed in basal medium for initial induction (first cultured in dark for 1.5 days , and then transferred to the condition of alternating light and dark for 5 days; wherein, the condition of alternating light and dark is: the light time is 13h / day, the dark time is 11h / day, the light intensity is 1900lux, the culture temperature is 22°C, and the relative humidity is 55%) to obtain callus;

[0041]2) The callus is placed in the induction medium for secondary induction (under the condition of alternating light and dark until the height of the adventitious bud exceeds 1 cm; wherein, the cond...

Embodiment 2

[0047] 1) Sterilize the leaves of succulent plants (Jade butterfly) (first soak the leaves in 60-70% by weight alcohol aqueous solution for 30-60s, then place them in 4% by weight sodium hypochlorite aqueous solution for 3.5min, and finally Washed 4 times by distilled water), then the leaves were chopped as explants (0.4 cm in length and 0.2 cm in width), and then the explant explants were placed in basal medium for initial induction ( First culture in the dark for 1 day, and then transfer to the condition of alternating light and dark for 4-6 days; wherein, the alternating light and dark conditions are: the light time is 12h / day, the dark time is 10h / day, the light intensity is 1900lux, and the culture temperature 21°C, 50% relative humidity) to obtain callus;

[0048] 2) Place the callus in the induction medium for secondary induction (under the condition of alternating light and dark until the height of the adventitious bud exceeds 1 cm; wherein, the condition of alternatin...

Embodiment 3

[0054] 1) Sterilize the leaves of succulent plants (Winter Beauty) (first soak the leaves in 70% by weight alcohol aqueous solution for 60s, then soak them in 5% by weight sodium hypochlorite aqueous solution for 4min, and finally wash them with distilled water for 5 minutes. times), then the leaves were minced as explants (0.45 cm in length and 0.3 cm in width), and then the explants were placed in basal medium for initial induction (first dark culture for 2 days , and then transferred to the condition of alternating light and dark for 6 days; wherein, the condition of alternating light and dark is: the light time is 14h / day, the dark time is 12h / day, the light intensity is 2000lux, the culture temperature is 23°C, and the relative humidity is 60%) to obtain callus;

[0055] 2) Place the callus in the induction medium for secondary induction (under the condition of alternating light and dark until the height of the adventitious bud exceeds 1 cm; wherein, the condition of alte...

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Abstract

The invention discloses a tissue culture method for a succulent plant. The method comprises the steps that 1, leaves of the succulent plant are sterilized and disinfected and then chopped as an explant, and the explant is placed in a basic culture medium for primary induction to obtain callus; 2), the callus is placed in an induction culture medium for secondary induction to promote generation ofat least two adventitious buds; 3, the callus with the adventitious buds is cut to obtain callus blocks, and the callus blocks are placed in a proliferation culture medium for proliferation culture toobtain proliferation bud blocks; 4, the proliferation bud blocks are placed in a rooting culture medium for rooting culture to obtain tissue culture seedlings; 5, the tissue culture seedlings are subjected to seedling hardening. The tissue culture method of the succulent plant has high survival rate and proliferation rate.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a tissue culture method for succulent plants. Background technique [0002] Succulents are also known as succulents and succulent plants. They are sometimes called succulents in gardening, but the name succulents is the most commonly used. Succulentsrefers to a certain part of the plant's vegetative organs, such as stems or leaves or roots a few species have two parts, a type of plant that has developed parenchyma to store water, and appears plump and succulent in appearance. [0003] The commonly used propagation methods of succulents are: grafting, cutting and sowing; no matter which of the above-mentioned propagation methods, there are defects of low survival rate and low reproduction efficiency, which limits the cultivation of succulents. Contents of the invention [0004] The object of the present invention is to provide a tissue culture method for succulent plants, which has ex...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 汪锡文裴宝平邱许凤
Owner 芜湖东源新农村开发股份有限公司
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