36 results about "Sugar transport" patented technology

The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.

An agent for increasing the sugar-transporting capacity and an agent for preventing and / or treating diabetes, diabetic peripheral neuropathy, diabetic nephropathy, diabetic retinopathy, diabetic macroangiopathy, impaired glucose tolerance or adiposis, which contains a lactam compound or a pharmaceutically acceptable salt thereof as the active ingredient.

The following fused polycyclic compound, analogues thereof or pharmaceutically acceptable salts thereof have an effect of increasing the sugar-transporting capacity and an effect of lowering the blood glucose level and, therefore, they are useful for preventing and / or treating diabetes and the like.wherein R is a methoxy group, Y is a (2-thiazolyl)-2-ethylcarbonyl group, R10 and R13 are hydrogen atoms, and R11 and R12 are methyl groups.

The present invention provides a lactam compound, a sugar transport enhancement agent containing this compound as an active ingredient, an agent for the prevention and / or treatment of diabetes mellitus, diabetic peripheral neuropathy, diabetic nephropathy, diabetic retinopathy, diabetic macrovascular disease, glucose tolerance anomaly, obesity and the like. In addition, the present invention also provides a preparation method for the novel lactam compound, and a preparation intermediate thereof.

The invention discloses a pear sugar transport protein gene PbSUT2 and application thereof. A nucleotide sequence of the pear sugar transport protein gene separated from a pear fruit is shown as SEQ ID No. 1, and a coded amino acid sequence of the pear sugar transport protein gene is shown as SEQ ID No. 2. The gene PbSUT2 is transformed into a tomato through an agrobacterium mediated genetic transformation method; the biological function verification of an obtained transgenic plant indicates that the cloned gene PbSUT2 has the functions of promoting plant early flowering and increasing the content of sucrose in the fruit. The discovery of the gene PbSUT2 provides new genetic resources for promoting the molecular breeding of plant fruit quality.

Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided are a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.

Disclosed are nucleic acid constructs comprising coding sequences operably linked to a promoter not natively associated with the coding sequence. The coding sequences encode Pichia stipitis proteins that allow recombinant strains of Saccharomyces cerevisiae expressing the protein to grow on xylose, and allow or increase uptake of xylose by Pichia stipitis or Saccharomyces cerevisiae expressing the coding sequences. Expression of the coding sequences enhances uptake of xylose and / or glucose, allowing increased ethanol or xylitol production.

The invention discloses two saccharose translocator ShSUT2 genes derived from sugarcane and application. According to the saccharose translocator genes ShSUT2A and ShSUT2B, amino acid sequences are respectively shown in SEQ ID NO 2 and 4, and base sequences of encoding genes ShSuT2A and ShSUT2B are respectively shown in SEQ ID NO 1 and 3. Absorbing function deficient mutant by yeast saccharose shows that the proteins encoded by ShSUT2A and ShSUT2B have saccharose transport activity. The ShSUT2A and ShSUT2B encoding genes are responsible for absorbing saccharose of apoplast in sugarcane plant and participate in transportation, distribution and accumulated physiological processes of sugarcane plant saccharose. The genes can provide new ways for improving transportation and distribution efficiency of sugarcane saccharose, promoting growth of the plant and improving sugarcane yields and sugar content.

The invention discloses a rice sugar transport gene OsVGT2 and a sugar transporter encoded by the rice sugar transport gene OsVGT2. The overexpression of the rice sugar transport gene OsVGT2 in rice can significantly increase the rice yield per plant, and the yield of a plot can be increased by 10-11 %, the seed setting rate and yield of rice can be significantly reduced after the mutation / knockout, and the yield of the plot can be reduced by 15‑18%; by affecting the transportation and distribution of photosynthetic products, it can affect the yield of rice, but has no effect on fertility; for Enhance the transport and distribution capacity of photosynthetic products through genetic improvement of varieties, and provide a realistic basis for continuous improvement of rice yield. The invention also discloses the application of the rice sugar transport gene OsVGT2 and its sugar transporter in cultivating high-yield crops, which can rapidly and effectively increase the yield of crops. The invention also discloses several PCR amplification primers of the rice sugar transport gene OsVGT2, which can effectively and quickly amplify the gene OsVGT2 from the rice cDNA.

This invention relates to the production of succinic acid and other chemicals derived from phosphoenolpyruvate (PEP) by fermentation with a microorganism in which the fermentation medium contains one or more sugars, and in which one or more of the sugars is imported into the cell by facilitated diffusion. As a specific example, succinic acid is produced from a glucose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize glucose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and / or a salt of succinic acid) at a commercial scale using feedstocks that include, for example, glucose, fructose, or sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of glucose or fructose into the parental strains. The genes involved in the transport and metabolism of glucose or fructose can also be introduced into a microorganism prior to developing the organism to produce a particular chemical. The genes involved in the transport and metabolism of sucrose can also be used to augment or improve the efficiency of sugar transport and metabolism by strains already known to have some ability for glucose utilization in biological fermentations.

This invention relates to the production of succinic acid and other chemicals derived from phosphoenolpyruvate (PEP) by fermentation with a microorganism in which the fermentation medium contains one or more sugars, and in which one or more of the sugars is imported into the cell by facilitated diffusion. As a specific example, succinic acid is produced from a glucose-containing renewable feedstock through fermentation using a biocatalyst. Examples of such a biocatalyst include microorganisms that have been enhanced in their ability to utilize glucose as a carbon and energy source. The biocatalysts of the present invention are derived from the genetic manipulation of parental strains that were originally constructed with the goal to produce one or more chemicals (for example succinic acid and / or a salt of succinic acid) at a commercial scale using feedstocks that include, for example, glucose, fructose, or sucrose. The genetic manipulations of the present invention involve the introduction of exogenous genes involved in the transport and metabolism of glucose or fructose into the parental strains. The genes involved in the transport and metabolism of glucose or fructose can also be introduced into a microorganism prior to developing the organism to produce a particular chemical. The genes involved in the transport and metabolism of sucrose can also be used to augment or improve the efficiency of sugar transport and metabolism by strains already known to have some ability for glucose utilization in biological fermentations.

Provided is a glucose derivative, which is taken into cells via a membrane sugar transport system and is represented by formula (1). Also provided are an imaging agent and an imaging method for a cell or intracellular molecule using said glucose derivative. Further provided is a method for detecting cancer cells with good accuracy using said glucose derivative and an imaging agent to be used in said method. More specifically provided are D-glucose derivatives and L-glucose derivatives in which glucose is bound to the 7-position of a fluorescent molecular group with a coumarin backbone or a quinolone backbone. Also provided are a cell imaging agent and imaging method using the derivative. A cancer cell imaging agent and imaging method using the L-glucose derivative is also provided. G is a group selected from formulas (G1)-(G4) below.

The invention discloses a pear sugar transport protein gene PbSUT2 and application thereof. A nucleotide sequence of the pear sugar transport protein gene separated from a pear fruit is shown as SEQ ID No. 1, and a coded amino acid sequence of the pear sugar transport protein gene is shown as SEQ ID No. 2. The gene PbSUT2 is transformed into a tomato through an agrobacterium mediated genetic transformation method; the biological function verification of an obtained transgenic plant indicates that the cloned gene PbSUT2 has the functions of promoting plant early flowering and increasing the content of sucrose in the fruit. The discovery of the gene PbSUT2 provides new genetic resources for promoting the molecular breeding of plant fruit quality.

The invention belongs to the field of gene engineering, and relates to a novel watermelon sugar transporter and a coding gene ClVST1 and application thereof. The novel watermelon sugar transport protein has a sequence composition shown in a sequence 1 in a sequence table, and the coding gene ClVST1 has a sequence composition shown in a sequence 2 in the sequence table. The invention also relates to application of the novel watermelon sugar transporter protein and the novel watermelon sugar transporter coding nucleic acid sequence in watermelon disease and insect pest resistance. The invention further provides an obtaining method of the watermelon plant disease and insect pest resistant strain. The watermelon novel sugar transporter and the coding gene ClVST1 provided by the invention are closely related to disease and insect pest resistance, and the higher the expression quantity is, the stronger the disease and insect pest resistance is; therefore, a stable watermelon strain resistant to bacterial fruit blotch and aphid can be obtained by knocking out novel sugar transport of watermelons in watermelon plants, and a quick and efficient way is provided for breeding of watermelons resistant to diseases and insect pests.

The invention discloses a block candy folding packaging machine, which comprises a frame, a sugar conveying mechanism, a sugar sorting mechanism, a sugar wrapping mechanism and a paper feeding device. The sugar conveying mechanism, the sugar sorting mechanism, the wrapping Sugar mechanism, sugar conveying mechanism includes sugar conveying motor and conveyor belt, sugar sorting mechanism includes sugar sorting motor, sugar dividing rod and temperature controller, sugar wrapping mechanism includes electrical control system, sugar pressing rod, packing table, first bag sugar rod, The second pack of sugar rods, the third pack of sugar rods, and the fourth pack of sugar rods; a paper feeding device is installed on the frame, and the paper feeding device corresponds to the bottom of the sugar wrapping mechanism, and the paper feeding device is connected with the paper storage box; the machine A control box is also installed in the frame, the control box is located below the paper storage box, and the control box is connected with the paper feeding device and the sugar wrapping mechanism. The present invention realizes the hexahedron folding packaging of block candies through the sugar conveying mechanism, the sugar sorting mechanism and the sugar wrapping mechanism, has a reasonable structure, avoids the sticking and accumulation of sugar blocks, and has high production efficiency.

The invention discloses a rice sugar transport gene OsMST1 and a sugar transporter encoded by the rice sugar transport gene OsMST1. Overexpression of the rice sugar transport gene OsMST1 in rice can significantly increase the yield of rice per plant, and the yield of a plot can be increased by 15-16 %, the seed setting rate and yield of rice can be significantly reduced after the mutation / knockout, and the yield of the plot can be reduced by 20‑22%; by affecting the transportation and distribution of photosynthetic products, it can affect the yield of rice, but has no effect on fertility; for Enhance the transport and distribution capacity of photosynthetic products through genetic improvement of varieties, and provide a realistic basis for continuous improvement of rice yield. The invention also discloses the application of the rice sugar transport gene OsMST1 and its sugar transporter in cultivating high-yield crops, which can rapidly and effectively increase the yield of crops. The invention also discloses several PCR amplification primers of rice sugar transport gene OsMST1, which can effectively and quickly amplify the gene OsMST1 from rice cDNA.

The invention discloses the application of the rice blast fungus gene MoHXT2 in regulating the plant sugar transport function. The present invention finds that the transporter encoded by MoHXT2 accepts a variety of sugars as substrates, that is, the MoHXT2 gene is a hexose transporter of Magnaporthe grisea. After knocking out MoHXT2 using the CRISPR / Cas9 gene knockout system, the melanin content of the mutant hyphae is found The pathogenicity was significantly reduced, and phlorizin could inhibit the transport of hexose by MoHXT2, thereby inhibiting the growth of Magnaporthe oryzae. Therefore, the MoHXT2 gene can be used to regulate plant sugar transport function, regulate the pathogenicity of Magnaporthe grisea and regulate the growth and development of Magnaporthe grisea, and it can also be used as a drug target for new pesticides, providing a more reliable direction for the synthesis of targeted fungicides .