Sugarcane monosaccharide transport protein ShHXT2 gene and application thereof

Abstract

The invention provides a sugarcane monosaccharide transport protein ShHXT2 gene of which the nucleotide sequence is shown as SEQ ID NO:1. The invention also provides a protein coded by the ShHXT2 gene and application of the ShHXT2 gene. According to the invention, the ShHXT2 gene is cloned from sugarcane for the first time, and the gene has a transport function of a sugar transport protein and can be positioned in the cell membrane of a cell; thus, the capability of the plant resistance against cold stress can be enhanced, the growth of a plant such as sugarcane is facilitated in an inverse condition, and a genetic basis is provided for revealing the regulation function of a sucrose transport mechanism on the growth development and quality improvement of the plant and a gene engineering research for increasing crop yield and improving quality.

Description

technical field [0001] The invention relates to the field of biological technology and relates to a sugarcane monosaccharide transporter ShHXT2 gene and its application. Background technique [0002] Sugarcane (Saccharum officinarum L.) belongs to the Poaceae Saccharum genus, which is divided into 9 species and originated in India and South Pacific islands. As one of the tropical crops, sugarcane is limited by temperature, mainly including: 34° north latitude - 31° south latitude, distributed in more than 80 countries and regions in the world. The annual sugarcane planting area reaches more than 15 million hectares worldwide, and the countries with the largest sugarcane planting area and the highest yield are India and Brazil respectively. There are also more than a dozen provinces and cities in China that plant sugar cane, mainly in Guangzhou, Yunnan, Guangxi, Hainan and Fujian. The growth cycle of sugarcane is generally 10-16 months, with high light saturation point, str...

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Problems solved by technology

At the same time, my country's sugarcane varieties are relatively single. However, in recent years, the quality of some fine varieties of sugarcane has gradually declined, and the yield has also decreased due to the impact of diseases and insect pests. Therefore, it is imminent to cultivate new varieties with high quality, high yield and stable yield.

However, due to the large number of chromosomes and complex genetics of sugarcane itself, it is difficult to achieve great improvement in traditional conventional breeding.

The genetic engineering method only changes the distribution of carbon substances, but does not increase the sugar content of sugarcane as a whole, so it is far from meeting the needs of production and life (Zhao Tingting, 2012)

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