Rice sugar transport gene osvgt2 and its sugar transporter, application and amplification primers

A technology for sugar transport and rice, which is applied in the field of rice sugar transport gene OsVGT2 and its sugar transporter, application and amplification primers, can solve the problems of high empty grain rate, poor transportation from source to reservoir, insufficient grain filling of upper grain, etc. To achieve the effect of increasing production and increasing production

Active Publication Date: 2022-01-25
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, "source" and "sink" are not the main limiting factors of high rice yield, but poor transportation from source to sink is the main reason for insufficient grain filling and high empty grain rate in rice production

Method used

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  • Rice sugar transport gene osvgt2 and its sugar transporter, application and amplification primers
  • Rice sugar transport gene osvgt2 and its sugar transporter, application and amplification primers
  • Rice sugar transport gene osvgt2 and its sugar transporter, application and amplification primers

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Embodiment 1

[0038] A rice sugar transport gene OsVGT2 , whose nucleotide sequence is shown in SEQ ID NO:1;

[0039] the sugar transporter gene OsVGT2and its sugar transporter OsVGT2 were discovered in rice through CRISPRE-CAS9 technology, as well as plant molecular genetics and physiological and biochemical methods. After research, we also found that its overexpression can significantly increase the yield of rice, and its gene OsVGT2 The mutation of the gene significantly reduces the seed-setting rate and yield of rice, which provides a realistic basis for improving the ability of photosynthetic product transportation and distribution through genetic improvement of varieties, and continuously improving rice yield.

[0040] 1. The sugar transport gene of the present invention OsVGT2 The spatiotemporal expression pattern of

[0041] In order to obtain the rice sugar transport gene OsVGT2 The expression pattern of the rice was sampled at different growth stages of rice for RNA extractio...

Embodiment 2

[0065] Rice sugar transport gene of the present invention OsVGT2 The application of its sugar transporter (sugar transporter OsVGT2) in cultivating high-yielding rice (improving rice seed setting rate and yield per plant), specifically including OsVGT2 Construction of overexpression and mutant plants, maturation phenotype and yield factor analysis.

[0066] In order to verify the role of the sugar transporter of the present invention in rice, we used CRISPR-Cas9 gene editing technology to edit OsVGT2, and obtained two knockout lines osvgt2-1 and osvgt2-2 with different mutation types, such as Figure 8 As shown, the mutation type of mutant osvgt2-1 is one base change and eight base deletions, and the mutation type of mutant osvgt2-1 is four base changes.

[0067] The content of soluble sugar and starch in each part of the wild type (WT) and mutants (osvgt2-1, osvgt2-2) at the filling stage was analyzed, as follows:

[0068] 1. Extraction method of total soluble sugar: 1) Tak...

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Abstract

The invention discloses a rice sugar transport gene OsVGT2 and a sugar transporter encoded by the rice sugar transport gene OsVGT2. The overexpression of the rice sugar transport gene OsVGT2 in rice can significantly increase the rice yield per plant, and the yield of a plot can be increased by 10-11 %, the seed setting rate and yield of rice can be significantly reduced after the mutation / knockout, and the yield of the plot can be reduced by 15‑18%; by affecting the transportation and distribution of photosynthetic products, it can affect the yield of rice, but has no effect on fertility; for Enhance the transport and distribution capacity of photosynthetic products through genetic improvement of varieties, and provide a realistic basis for continuous improvement of rice yield. The invention also discloses the application of the rice sugar transport gene OsVGT2 and its sugar transporter in cultivating high-yield crops, which can rapidly and effectively increase the yield of crops. The invention also discloses several PCR amplification primers of the rice sugar transport gene OsVGT2, which can effectively and quickly amplify the gene OsVGT2 from the rice cDNA.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to rice sugar transport genes OsVGT2 and its sugar transporters, applications and amplification primers. Background technique [0002] Rice is one of the most important food crops in the world, and it is also the largest food crop in my country. Its sown area accounts for about 28% of my country's grain crop sown area, and rice production accounts for more than 40% of my country's total grain output. About 60% of the country's population uses rice as a staple food (Cheng Shihua, 2005). Faced with multiple pressures such as population expansion, sharp decline in arable land, and resource shortages, my country's rice production situation is grim. Therefore, continuous improvement of rice yield is still the primary direction of rice genetics and breeding. [0003] After dwarf breeding and utilization of heterosis contributed to the first and second leaps in rice yield, how to further in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N15/11A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 张振华刘冬罗劲松
Owner HUNAN AGRICULTURAL UNIV
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