Rice sugar transport gene osmst1 and its sugar transporter, application and amplification primers

A sugar transfer and rice technology, applied in the introduction of foreign genetic material using vectors, applications, genetic engineering, etc., can solve the problems of high empty seedling rate, poor source-to-storage transportation, and insufficient grain filling, and achieve the goal of increasing yield. Effect

Active Publication Date: 2022-02-25
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, "source" and "sink" are not the main limiting factors of high rice yield, but poor transportation from source to sink is the main reason for insufficient grain filling and high empty grain rate in rice production

Method used

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  • Rice sugar transport gene osmst1 and its sugar transporter, application and amplification primers
  • Rice sugar transport gene osmst1 and its sugar transporter, application and amplification primers
  • Rice sugar transport gene osmst1 and its sugar transporter, application and amplification primers

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Effect test

Embodiment 1

[0036] A rice sugar transport gene OsMST1 , whose nucleotide sequence is shown in SEQ ID NO:1;

[0037] the sugar transporter gene OsMST1 The sugar transporter OsMST1 was discovered in rice through CRISPRE-CAS9 technology, as well as plant molecular genetics and physiological and biochemical methods. After research, we also found that its overexpression can significantly increase the yield of rice, and its gene OsMST1 The mutation of the gene significantly reduces the seed-setting rate and yield of rice, which provides a realistic basis for improving the transportation and distribution of photosynthetic products through genetic improvement of varieties, and continuously improving the yield of rice.

[0038] In order to verify the sugar transport gene of the present invention OsMST1and the function of its sugar transporter OsMST1, the following experiments were carried out:

[0039] 1. The sugar transport gene of the present invention OsMST1 The spatiotemporal expression p...

Embodiment 2

[0064] Rice sugar transport gene of the present invention OsMST1 The application of its sugar transporter (sugar transporter OsMST1) in cultivating high-yielding rice (increasing rice seed setting rate and yield per plant), specifically including OsMST1 Construction of overexpression and mutant plants, maturation phenotype and yield factor analysis.

[0065] In order to verify the sugar transport gene of the present invention OsMST1 In rice, we use CRISPR-Cas9 gene editing technology to OsMST1 Edited to obtain two knockout lines with different mutation types osmst1-1 , osmst1-2 ,Such as Figure 4 As shown, the knockout strain osmst1-1 The mutation type is 5 base deletions, knockout strains osmst1-2 The type of mutation is a deletion of 4 bases.

[0066] And for wild type (WT) and mutant ( osmst1-1 , osmst1-2 ) analysis of soluble sugar and starch content in each part during the grouting period, as follows:

[0067] 1. Extraction method of total soluble sugar: 1...

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Abstract

The invention discloses a rice sugar transport gene OsMST1 and a sugar transporter encoded by the rice sugar transport gene OsMST1. Overexpression of the rice sugar transport gene OsMST1 in rice can significantly increase the yield of rice per plant, and the yield of a plot can be increased by 15-16 %, the seed setting rate and yield of rice can be significantly reduced after the mutation / knockout, and the yield of the plot can be reduced by 20‑22%; by affecting the transportation and distribution of photosynthetic products, it can affect the yield of rice, but has no effect on fertility; for Enhance the transport and distribution capacity of photosynthetic products through genetic improvement of varieties, and provide a realistic basis for continuous improvement of rice yield. The invention also discloses the application of the rice sugar transport gene OsMST1 and its sugar transporter in cultivating high-yield crops, which can rapidly and effectively increase the yield of crops. The invention also discloses several PCR amplification primers of rice sugar transport gene OsMST1, which can effectively and quickly amplify the gene OsMST1 from rice cDNA.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to rice sugar transport genes OsMST1 and its sugar transporters, applications and amplification primers. Background technique [0002] Rice is one of the most important food crops in the world, and it is also the largest food crop in my country. Its sown area accounts for about 28% of my country's grain crop sown area, and rice production accounts for more than 40% of my country's total grain output. About 60% of the country's population uses rice as a staple food (Cheng Shihua, 2005). Faced with multiple pressures such as population expansion, sharp decline in arable land, and resource shortages, my country's rice production situation is grim. Therefore, continuous improvement of rice yield is still the primary direction of rice genetics and breeding. [0003] After dwarf breeding and utilization of heterosis contributed to the first and second leaps in rice yield, how to further in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82C12N15/11A01H5/00A01H6/46
CPCC07K14/415C12N15/8261
Inventor 张振华刘冬罗劲松
Owner HUNAN AGRICULTURAL UNIV
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