Transformed plant, method for producing sugar-containing exudate using transformed plant

A manufacturing method, a technology for exudates, applied in the directions of angiosperms/flowering plants, chemical instruments and methods, biochemical equipment and methods, etc., to achieve the effect of increasing sugar content

Active Publication Date: 2019-03-22
TOYOTA JIDOSHA KK +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Thus, conventionally, the production of raw material sugar using plants such as sugarcane requires huge costs such as the cost of the manufacturing process and the cost of cultivation.

Method used

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  • Transformed plant, method for producing sugar-containing exudate using transformed plant
  • Transformed plant, method for producing sugar-containing exudate using transformed plant
  • Transformed plant, method for producing sugar-containing exudate using transformed plant

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Embodiment

[0224] Hereinafter, the present invention will be described in more detail using examples, but the technical scope of the present invention is not limited to these examples.

[0225] 1. Preparation of DNA constructs for transformation of Arabidopsis thaliana

[0226] 1.1 Obtain DNA encoding AtSWEET protein by PCR

[0227] 1.1.1 Amplification of DNA encoding AtSWEET protein

[0228] DNAs encoding AtSWEET1, AtSWEET2, AtSWEET3, AtSWEET4, AtSWEET5, AtSWEET6, AtSWEET7, AtSWEET9, AtSWEET11, AtSWEET12, AtSWEET13, AtSWEET15, and AtSWEET17 proteins for evaluation were amplified by PCR using cDNA prepared from Arabidopsis thaliana as a template. In order to insert the DNA for evaluation into the pRI201AN vector (manufactured by Takara Bio, #3264), a forward primer with a Sal I restriction enzyme recognition sequence added to the 5' end and a Sac I or Pst I added to the 3' end was designed. Reverse primers for restriction enzyme recognition sequences (Table 6).

[0229] Table 6

[02...

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Abstract

Exudates containing high concentrations of sugars are produced from plants. A nucleic acid encoding a transporter involved in sugar transport having a defined consensus sequence derived from the amino acid sequence of a SWEET protein classified as clade III is introduced and / or expression of the protein is enhanced.

Description

technical field [0001] The present invention relates to a transformed plant having excellent properties obtained by introducing a predetermined gene and a method for producing sugar-containing exudates using the transformed plant. Background technique [0002] The stable production of biofuels and bioplastics requires a cheap and stable supply of raw material sugar. The most representative raw material sugar is the sugar accumulated in sugarcane. In order to extract sugar from sugar cane, it is generally necessary to harvest sugar cane at a predetermined harvest time, and perform crushing, pressing, concentration, purification, and other treatments. In addition, the harvested field requires management operations such as field maintenance for new cultivation, planting, and spraying of herbicides and / or insect repellents. Thus, conventionally, production of raw material sugar using plants such as sugar cane requires huge costs such as production process costs and cultivation...

Claims

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Application Information

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IPC IPC(8): A01H6/00C12N5/10C12N15/09
CPCC07K14/415C12N15/8245
Inventor 大音德米仓円佳青木直大大杉立广濑龙郎
Owner TOYOTA JIDOSHA KK
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