51 results about "Stress inducible" patented technology

The present invention discloses and claims stress-inducible plant promoters, DNA constructs with such promoters, transgenic plants containing such promoters and DNA constructs, isolated DNAs encoding such promoters, and methods of making and using such promoters, DNA constructs, transgenic plants, and isolated DNAs.

A genetic construct is taught which contains a nitrogen assimilation and / or metabolism gene and a promoter for the gene which is inducible under conditions where it would be beneficial to take-up, store or use nitrogen. The promoter can be, for example, induced by the presence of nitrate or other form of nitrogen and will be induced by application of a nitrogenous fertilizer. The stress inducible promoter btg-26 is also taught. Promoter btg-26 is isolated from the Brassica turgor gene-26 and exhibits osmotic-stress induced expression.

The invention discloses a thellungiella V-pyrophosphatase gene (TsVPI) promoter sequence and the application of a deletion mutant thereof. A clone carrying a TsVPI promoter is screened from a genomiclibrary of halophyte thellungiella, a 2200 nucleotide sequence positioned on the upstream of a TsVPI coding frame 5' is truncated as an overall-length promoter, promoter snippets with different lengths are obtained through PCR amplification, and then the promoter snippets are respectively blended with gus genes to be recombined into a plant expression vector to be converted into Arabidopsis; the TsVPI promoter and a part of the deletion mutant thereof are determined as salt-stress inducible type promoters by detecting the GUS enzymatic activity of transgenic plants, wherein a T5 promoter not only has a short sequence (667bp) but also has root specificity, and the TsVPI promoter and the T5 promoter are respectively connected with betA genes from colibacillus to be transmitted into tobaccosand corns so as to determine that the TsVPI promoter and the T5 promoter can normally exert functions in the transgenic tobaccos and the transgenic corns, are salt-stress inducible type strong promoters and have important application value in the industrialization development of plant gene engineering.

The invention discloses a plant inducible promoter and an application thereof. A section of HbCIPK2 promoter sequence of 1,750bp is obtained from a halophyte wild barley genome through a chromosome primer walking method, an arabidopsis proHbCIPK2:GUS strain obtained through the promoter sequence can drive expression of GUS genes under stress of different concentrations and times, and the expression activity of GUS is enhanced along with increase of the stress times and concentrations. The inducible expression activity of the GUS genes driven by the HbCIPK2 promoter under PEG and ABA stress is relatively low. The identical transgenic lines are free of GUS gene expressions under stress-free treatment and low-temperature and high-temperature stress. The obtained DNA sequence of 1,750 bp is the novel stress inducible promoter, and has high promoter activity under high-salt stress, and proper expressions of transcription factors and other controlling genes can be induced.

The invention provides an OsGRAS1 gene related to rice stress resistance, its promoter and application thereof. The gene contains the conserved domain of the GRAS gene family and is a member of the SCL type of the gene family. The expression of OsGRAS1 gene is induced by drought, salt and exogenous ABA, and it is related to the stress resistance of rice. The difference in the promoter sequence of OsGRAS1P caused the response time and expression level of the downstream OsGRAS1 gene to be upregulated by drought, salt and exogenous ABA to change. The promoter can be used as a gene regulatory element induced by stress. The rice gene and its promoter of the invention produce obvious response to adversity, and can be applied to stress resistance breeding of plants to improve the stress resistance of plants.

The present invention relates to the technical field of immunology and the treatment of diseases mediated and / or indicated by the presence of a ligand of NK receptor NKG2D, such as major histocompatibility complex class I chain related (MIC) A and B molecules on the cell surface of diseased tissue or cells. In particular, the present invention makes use of the surprising finding that the stress-inducible endogenous danger signals HSP70 and MICA / B synergistically activate NK cells against tumors. Methods and compositions for the prevention and treatment of infectious diseases, primary and in particular metastatic neoplastic diseases, including, but not limited to human sarcomas, carcinomas and melanomas are provided.

The invention discloses a promoter of which the sequence is as shown in SEQ ID NO:1 in a sequence table. The promoter can be used for culturing transgenic plants, i.e., the transgenic plants containing the promoter and target genes are obtained by firstly, establishing a recombinant expression carrier by using the promoter and the target genes, subsequently infecting a transformant by using the recombinant expression carrier, further transforming target plants by using the transformant, and detecting. Due to the osmotic stress inducible characteristic, the promoter disclosed by the invention can be used for connecting relevant anti-retroviral target genes, used for promoting the expression of the relevant anti-retroviral target genes when plants are subjected to osmotic stress, and used for improving the capability of the plants to adversity stress, and a powerful tool is provided for the plant stress resistance gene engineering.

The present invention discloses and claims stress-inducible plant promoters, DNA constructs with such promoters, transgenic plants containing such promoters and DNA constructs, isolated DNAs encoding such promoters, and methods of making and using such promoters, DNA constructs, transgenic plants, and isolated DNAs.

The invention discloses a method for regulating expression of a heterologous nucleotide sequence in plants and a DNA carrier. The DNA carrier comprises the nucleotide sequence of a new plant stress inducible gene promoter. The invention provides the method for expressing the heterologous nucleotide sequence in plants by using the sequence of the stress inducible gene promoter disclosed by the invention.

The present invention relates to an AtSIK protein functioning as a negative regulator to the osmotic stress-inducible gene and more particularly to a gene encoding said protein. Said AtSIK protein isolated from Arabidopsis thaliana acts as a negative regulator to the osmotic stress-inducible gene. Therefore, a plant having enhanced resistance to osmotic stress can be produced by inhibiting expression of AtSIK and consequently, the productivity of the plant can be increased.

Compositions and methods for detection of the stress-inducible Hsp70B′ protein are disclosed. These include antibodies directed against particular amino acid regions of Hsp70B′ and various peptides corresponding, or antigenically equivalent, to the regions. The ability to generate anti-Hsp70B′ antibodies to defined epitopes permits a variety of in vitro and in vivo uses.

The invention relates to siRNA based on Sitobion avenae SaveOrco gene design and application thereof.Transcription of a SaveOrco gene is interfered through feeding of siOrco7.Real-time fluorescent quantitative polymerase chain reaction (PCR) research shows that the expression of the SaveOrco gene is obviously restrained.Olfactory behavior reaction detection results show that the responding rates of silenced aphids to z-3-hexenol, methyl salicylate and EBF are obviously reduced under SaveOroco expression.An EBF inducing experiment for sitobion avenae winged morph differentiation is developed, siRNA processed aphids stop inducing of EBF, normal chemical communication of the aphids is interfered, and stress inducible wing bud generation is also stopped.In this way, a conserved sequence of the Orco gene can be applied to an RNAi technology mediated by siRNA, and the aphids can be effectively restrained from rapidly diffusing to be harmful and are prevented and treated.

The invention provides a nucleic acid construct for expressing an oxidative stress indicator, comprising a nucleic acid sequence encoding a partial protein, which contains at least the Neh2 domain sequence and is obtained by substantially deleting or functionally deleting the Neh1 domain sequence or the Neh1-Neh3 domain sequence in Nrf2 protein, a stress-inducible promoter sequence, which is located upstream the nucleic acid sequence, and a nucleic acid sequence, which encodes a protein capable of emitting a detectable signal and is located downstream the nucleic acid sequence. The invention also provides a method for measuring oxidative stress and a method for screening an oxidative stress inhibitor, each using the nucleic acid construct.

The invention relates to a physarum polycephalum stress-inducible protein 1 (PSTI1) for improving stress resistance of a prokaryote and application of the PSTI1, and in particular provides a PSTI1 separated from physarum polycephalum of eucaryon and coded genes and application of the product in improvement of stress resistance of the prokaryote. Experiments prove that the over-expressed PSTI1 in the prokaryote can widely improve the salt, osmotic pressure, heavy metal ion, oxidation, oxygen deficiency and pH change stress tolerance of the prokaryote and the PSTI1 can widely affect the stress response function of prokaryotic cells. Therefore, the PSTI1 can be used for converting the prokaryote so as to improve the environmental pollution treatment capacity of the prokaryote such as bacteria and the like, and can also be used for improving the production efficiency of bioengineering bacteria used in the field of biotechnology.

The invention which belongs to the technical field of biological engineering provides a Zea mays adverse stress inducible promoter sequence. The adverse stress inducible promoter sequence is characterized in that: the promoter sequence comprises DNA nucleotide sequences relative to a zone from -1bp to -1150bp at the transcription start site of SEQ ID NO: 1. The invention also provides an adverse stress inducible plant expression vector for Zea mays transformation. The plant expression vector comprises the Zea mays adverse stress inducible promoter and a 5' untranslated region of ZmapH (Zea mays anionic peroxidase H); and PCR primers of SEQ ID NO:2 and SEQ ID NO:3 are suitable for the amplification of DNA segments containing the SEQ ID NO:1. The Zea mays adverse stress inducible promoter can be used to promote adverse stress resistant gene to efficiently express, and has a positive meaning to the research of the transgenic technology of the Zea mays stress tolerant species and the cultivation of the strong comprehensive resistance high quality high yield new transgenic species.

The present invention relates to transgenic plants comprising an isolated DNA molecule comprising a polynucleotide that encodes a nucleic acid that down-regulates an endogenous T6PP gene, wherein the polynucleotide is under the control of a promoter that is stress-inducible and is expressed predominantly in vegetative tissue. The promoter may also be developmentally expressed in maturing kernels. Expression of the polynucleotide results in the increased availability of carbon to developing florets / kernels when plants are subject to environmental stress, such as a water deficit. The DNA molecule of the invention thereby permits more photosynthate to be directed to the developing ovules / embryos resulting in stabilized yield in growing environments that are subject to periodic stress.

A corn stress-inducible promoter and activity analysis belong to the technical field of bioengineering. The invention provides an obtained corn stress-inducible promoter sequence, a stress-inducible plant expression vector for corn transformation and genetically modified plants transformed by plant expression vector; the corn stress-inducible promoter sequence contains a DNA (deoxyribonucleic acid) nucleotide sequence from the -1bp region to the -1299bp region relative to the transcription start site of SEQ ID NO: 1; the stress-inducible expression vector contains the corn stress-inducible promoter sequence and a 5' untranslated region of a corn DRE-binding protein gene; the genetically modified plants transformed by the plant expression vector contain the corn stress-inducible promoter sequence and the 5' untranslated region of the corn DRE-binding protein gene; and PCR (polymerase chain reaction) primers of SEQ ID NO: 2 and SEQ ID NO: 3 are suitable for amplifying the sequence DNA segment containing the SEQ ID NO: 1. The corn stress-inducible promoter can be used for promoting the high-efficiency expression of drought-resistant genes and in drought-resistant genetically modifiedplants, and has a positive significance in solving grain crisis in arid regions and increasing grain yield.

This invention provides a stress-inducible promoter that effectively functions in monocotyledonous plants such as rice, and environmental stress-tolerant plants using the promoter. Such promoter is derived from rice and consists of the following DNA (a) or (b): (a) DNA that consists of the nucleotide sequence as shown in SEQ ID NO: 1 or 10; or (b) DNA that hybridizes under stringent conditions with DNA consisting of a nucleotide sequence that is complementary to the DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 1 or 10 and that expresses stress-inducible promoter activity. Such environmental stress-tolerant plant has had such promoter introduced therein.

The invention belongs to the technical field of molecular biology and gene engineering, and provides a promoter used for corn low phosphor stress inducible expression, and applications thereof. The promoter is used for solving problems in the prior art that transgenic plant nutrient and energy are over consumed, plant growth and yield are influenced, and phosphorus poisoning is caused because of high expression of a conventional combined promoter under any phosphor conditions. The promoter comprises a ZmPHR1 gene initiation codon ATG upstream 2205bp promoter sequence represented by SEQ ID NO:1, and 5'-terminal deleted promoter deletion fragment sequences represented by SEQ ID NO:2, 3, 4, and 5. The promoter can be used for replacing the combined promoter in plant engineering, ensuring thefunction of phosphor high efficiency absorption and utilization genes under low phosphor stress, avoiding energy waste caused by unnecessary expression at normal phosphor conditions, and culturing ideal high phosphor crop varieties.

The invention discloses a dendrobium officinale flower tissue preference and stress inducible promoter ProDoWOX4 and application thereof, and belongs to the technical field of molecular biology. The promoter ProDoWOX4 provided by the invention has the length of 2003bp and can induce preference expression of a corresponding target gene in sepals and petals of floral organs, and due to the dominant expression, consumption of other resources and energy of plants can be reduced, and an appropriate regulation and control element is provided for construction of a plant genetic engineering expression vector; and the promoter has important significance on improvement and improvement of excellent characters such as salt tolerance or cold tolerance of plants, so that the breeding process of excellent varieties can be accelerated. The invention also provides a nucleic acid construct containing the promoter ProDoWOX4, a recombinant expression vector, a host cell, a genetic engineering cell and an amplification primer, and application of the substances.

The invention belongs to the field of plant gene engineering and particularly relates to separation, clone and an abiotic stress (drought, high salt and abscisic acid ABA) inducible prompter OsIP3KP obtained through functional verification. The promoter has a nucleotide sequence expressed by SEQ ID NO: 1 and has a sequence length of 2119 bp. Through the promoter, the specific expression of an anti-stress related gene in the abiotic stress environment can be improved, and the promoter is applied to the genetic improvement on the abiotic stress resisting capacity of rice. According to the invention, the rice-controlling abiotic stress inducible promoter OsIP3KP is cloned by adopting a PCR (Polymerase Chain Reaction) amplification and fusion report gene genetic transformation method; and through realtime-PCR, reporter gene GUS (Glucuronidase) staining observation and enzyme activity assay, the cloned prompter is proved to be induced by the abiotic stress (drought, high salt and abscisic acid ABA) and can be applied to the genetic improvement on rice and other plants.

This present invention relates to isolation and derivation of nucleic acid sequences from monocot plants, preferably rice that are capable of driving and / or regulating a stress induced expression of an operably linked nucleic acid. The present invention also is directed to the use of the isolated nucleic acid to drive and / or regulate a stress-induced expression of an operably linked nucleic acid. The isolated nucleic acid sequence of the present invention as set forth in SEQ ID NO 3 or SEQ ID NO 6 or SEQ ID NO 7 or SEQ ID NO 9 or the complement thereof can be an inducible promoter. The promoters of the invention can be induced by abiotic stress such as water, cold, heat and / or salinity or a biotic stress such as by a virus, bacteria, or fungi.

The present invention discloses and claims stress-inducible plant promoters, DNA constructs with such promoters, transgenic plants containing such promoters and DNA constructs, isolated DNAs encoding such promoters, and methods of making and using such promoters, DNA constructs, transgenic plants, and isolated DNAs.

Compositions and methods for detection of the stress-inducible Hsp70B′ protein are disclosed. These include antibodies directed against particular amino acid regions of Hsp70B′ and various peptides corresponding, or antigenically equivalent, to the regions. The ability to generate anti-Hsp70B′ antibodies to defined epitopes permits a variety of in vitro and in vivo uses.

This invention concerns a diagnostic system made of: a transformed microorganism capable of an increased reporter activity upon exposure to an environmental insult, said microorganism having a stress inducible promoter sequence being operatively linked to a reported encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed, and of a transformed microorganism having a constitutive and non stress inducible promoter sequence being operatively linked to a reporter encoding nucleic acid sequence encoding a reporter molecule resulting in a signal that can be assayed.

The present invention provides a method for inhibiting secretase activity. A method for promoting the sensitivity of a secretase inhibitor, a method for binding synoviolin to Herp (homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1), or the like is employed to inhibit secretase activity.

The invention belongs to the field of genetic engineering, and in particular relates to a salt stress inducible promoter as well as primers, an expression vector and application thereof. A promoter fragment is cloned, a vector of the promoter fragment is constructed, arabidopsis genetic transformation is performed, an arabidopsis resistant material is screened and detected, GUS staining is carriedout, and RT-qPCR is used for detecting the expression situation of a GUS gene. The results show that the promoter fragment provided by the invention can drive the expression of the GUS gene and can be expressed in roots, stems, leaves, flowers and other parts; under the condition of abiotic stress induction, the activity and driving efficiency of the promoter are also higher than those under thenormal conditions; the qPCR results also show the similar results, so that the promoter provided by the invention can improve the salt tolerance of transgenic crops; therefore, the salt stress inducible promoter has a wide application value.

The invention provides a rice exogenous stress-induced expression promoter Psubs3 and application thereof. The sequence of the inducible expression promoter is the nucleotide sequence shown in SEQ ID No: 1, and the present invention also provides another external cause inducible expression promoter, the sequence of which is shown in SEQ ID No: 2. Specifically, the present invention applies the above-mentioned promoters to plant transgenic engineering. The promoter provided by the present invention can initiate the expression of exogenous genes in plants under submerged or salty environments, and is suitable for any plant, especially capable of driving the induced expression of exogenous genes in rice plants, so it can be used to improve and improve The growth characteristics of rice in flooded environment, so as to cultivate ideal rice varieties.

The invention relates to siRNA designed based on the conservative olfactory receptor Orco gene of sitobion avenae and an application thereof. In the invention, siOrco8 is fed to interfere with the transcription of the SaveOrco gene. Real-time fluorescent quantitative PCR study indicates that the expression of the SaveOrco gene is obviously inhibited. The olfactory behavior reaction detection result shows that the responsivity of aphids with expression loss of SaveOrco to cis-3-hexenol, methyl salicylate and EBF is remarkably reduced. In an experiment of EBF inducing wing dimorphism of sitobion avenae, the aphids treated with siRNA stop induction of EBF, normal chemical communication of the aphids is disturbed, and the stress-inducible wing bud generation is also stopped. Therefore, the conservative sequence of the Orco gene can be used for effectively preventing aphids from quickly spreading and doing harm through the siRNA-mediated RNAi technology so as to control aphids.