90 results about "Streptomyces thioluteus" patented technology

The invention discloses a streptomyces parvus and application thereof for preparing daptomycin. The strain is reserved in the CCTCC and has the reservation number of CCTCC NO: M2010136, and the reservation data is June 4, 2010. The method for preparing the daptomycin is as follows: a, taking streptomyces parvus CCTCC NO: M2010136 as a fermentation strain; b, strain cultivation: inoculating slope lawn into a shake flask seeding tank to cultivate to obtain shake flask seeding liquid; inoculating the shake flask seeding liquid into the seeding tank to be cultivated; inoculating the seeding tank culture solution into fermentation tank culture medium to be cultivated, and collecting fermentation liquor; carrying out feed supplement in the fermentation and cultivation process; and c, extracting a fermentation product. The fermentation technology provided by the invention has stable production capability, high fermentation unit and few fermentation byproducts by the pilot plant test and the experiment in a 10-ton fermentation tank, greatly lowers post-extracting difficulty and is suitable for industrial production.

In accordance with the present invention, a novel aromatic prenyltransferase, Orf2 from Streptomyces sp. strain CL190, involved in naphterpin biosynthesis has been identified and the structure thereof elucidated. This prenyltransferase catalyzes the formation of a C—C bond between a prenyl group and a compound containing an aromatic nucleus, and also displays C—O bond formation activity. Numerous crystallographic structures of the prenyltransferase have been solved and refined, e.g., (1) prenyltransferase complexed with a buffer molecule (TAPS), (2) prenyltransferase as a binary complex with geranyl diphosphate (GPP) and Mg²⁺, and prenyltransferase as ternary complexes with a non-hydrolyzable substrate analogue, geranyl S-thiolodiphosphate (GSPP) and either (3) 1,6-dihydroxynaphthalene (1,6-DHN), or (4) flaviolin (i.e., 2,5,7-trihydroxy-1,4-naphthoquinone, which is the oxidized product of 1,3,6,8-tetrahydroxynaphthalene (THN)). These structures have been solved and refined to 1.5 Å, 2.25 Å, 1.95 Å and 2.02 Å, respectively. This first structure of an aromatic prenyltransferase displays an unexpected and non-canonical (β/α)-barrel architecture. The complexes with both aromatic substrates and prenyl containing substrates and analogs delineate the active site and are consistent with a proposed electrophilic mechanism of prenyl group transfer. These structures also provide a mechanistic basis for understanding prenyl chain length determination and aromatic co-substrate recognition in this structurally unique family of aromatic prenyltransferases. This structural information is useful for predicting the aromatic prenyltransferase activity of proteins.

The present invention provides cloning sequencing, analyzing, function research of a biosynthesis gene cluster of an antibiotic-Azinomycin B having antitumor activity produced by streptomyces, and its application. The whole gene cluster includes 34 genes: one repeatedly using I type polyketide synthase gene; two naphthalene ring modification enzyme genes; 8 non-ribosomal polypeptide skeleton synthesis and modification enzyme genes; 11 non-natural amino acid structure unit synthase genes; 1 resistance gene; 3 post modification enzyme genes and 8 genes which functions are not determined. The genetic operation of the biosynthesis gene breaks the synthesis of Azinomycin B; the precursor compound is produced by the heterologous expression of synthesis gene and modification gene of naphthalene ring. The gene of the invention and the protein can be used for searching and finding compound or gene, protein applied in medical, industry or agriculture.

This invention relates to a new class of polyene polyketides, their pharmaceutically acceptable salts and derivatives, and to methods for obtaining the compounds. One method of obtaining these compounds is by cultivation of novel strains of Streptomyces aizunensis; another method involves expression of biosynthetic pathway genes in transformed host cells. The present invention further relates to the novel strains of Streptomyces aizunensis used to produce these compounds, to the use of these compounds and their pharmaceutically acceptable salts and derivatives as pharmaceuticals, in particular to their use as inhibitors of fungal cell growth and cancer cell growth. The invention also relates to pharmaceutical compositions comprising these novel polyketides or a pharmaceutically acceptable salts or derivatives thereof. Finally, the invention relates to novel polynucleotide sequences and their encoded proteins, which are involved in the biosynthesis of these novel polyketides.

The invention relates to an nsdBmgh gene of Streptomyces roseoflavus Men-myco-93-63 as well as a preparation method and application thereof. In the invention, primers Ns8F and Ns8R are designed by utilizing an important negative regulator gene nsdB sequence in a Streptomyces coelicolor A3(2) antibiotic metabolic pathway, and genomic DNA (deoxyribonucleic acid) of Streptomyces roseoflavus Men-myco-93-63 is amplified to obtain a 1121bp segment; and two ends of a target sequence are extended by utilizing a primer anchoring polymerase chain reaction) (NPA-PCR ) method by a non-specific primer, thus obtaining the nsdBmgh gene of Streptomyces roseoflavus Men-myco-93-63. The preparation of the nsdBmgh gene provides an important basis for further researching and verifying the Streptomyces roseoflavus antibiotic metabolic pathway and regulatory mechanism, and establishes an important base for obtaining high-yield antibiotic gene engineering strains.

The invention discloses a recombined streptomycete, a construction method of the recombined streptomycete and a method for increasing the antibiotic yield. The recombined streptomycete over-expresses acetolactic acid synthase small subunit ilvH. The recombined streptomycete over-expresses acetolactic acid synthase to adjust subunit genes, and the yield of products such as antibiotics for conducting secondary metabolism with L-isoleucine and/or L-valine and/or L-leucine as precursors/a precursor can be remarkably increased.

The present invention provides a tomaymycin biosynthetic gene cluster of Streptomyces species FH6421, and its use for producing 11-de-O-methyltomaymycin.

The invention provides a gene engineering strain Streptomyces chattanoogensis. The preservation registration number of the gene engineering strain Streptomyces chattanoogensis is CGMCC No.7182, the nucleotide sequence of the gene engineering strain Streptomyces chattanoogensis is represented by SEQ ID NO.1, and the gene engineering strain Streptomyces chattanoogensis is obtained through the genetic construction of a rate-limiting enzyme in the biosynthetic way of natamycin. Compared with gene engineering strain Streptomyces chattanoogensis L10, the gene engineering strain Streptomyces chattanoogensis L11 has the advantages of more stable hereditary characters, improvement of the natamycin fermentation unit to 3.12g/L from 2.21g/L, shortening of the natamycin fermentation period to 120h from 144h, improvement of the natamycin output by 40%, shortening the fermentation period by 24h, and reduction of the industrialized production process, and can be applied to the generation of natamycin.

The invention discloses streptomyces thioluteus St-79, and the preservation number of the streptomyces thioluteus St-79 is CCTCC (China Center for Type Culture Collection) NO: M 2020962. The application of the streptomyces thioluteus St-79 strain is as follows: the germination of rice seeds is promoted, the bacterial blight of rice is prevented and treated, and the pathogenic bacteria of plants are inhibited.

The invention relates to a formula for preparing a high-efficiency bio-organic fertilizer from organic materials and a preparation method of the high-efficiency bio-organic fertilizer. The preparation method comprises the steps: firstly, fully mixing livestock manure with auxiliary materials including peanut meal, humic acid, enzyme microorganisms, fishbone dust, soybean leaf powder and amino acid; adding bacillus amyloliquefaciens, bacillus megaterium, bacillus mucilaginosus, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, azotobacter chroococcum, actinomycetes, photosynthetic bacteria flora, lactic acid bacteria flora, saccharomycetes, bacillus coagulans, aspergillus oryzae, aspergillus niger and trichoderma viride, coarsely adjusting the moisture content and carbon nitrogen ratio of the materials according to the raw material components, mixing and fermenting; and thus obtaining the bio-organic fertilizer which does not have pesticide residues and naturally improves the crop yield.

The invention discloses a salinomycin strain with the class name of streptomycesalbums S-610-11, and the preservation number is CCTCCM2012492. The salinomycin high-yielding strain streptomycesalbums S-610-11 is obtained by combination of plasma mutagenesis at normal temperature under normal pressure with high-throughput synergy screening method, the content of salinomycin can be greatly improved, and the stability is good, after 5 times of continuous passages of the strain, and the salinomycin titer has no obvious change. By use of shake flask fermentation, the yield of salinomycin reaches 56000u / ml, and is improved by 70% compared with that of original strain F2-1#. The salinomycin strain is fully consistent with mutagenesis and screening requirements of industrialization salinomycin production strains, and can be applied in industrial fermentation production, and has great economic value.

The invention provides a method for constructing a high-yield neomycin engineering strain streptomyces fradiae. The method comprises: introducing a neomycin biosynthetic gene cluster of streptomyces fradiae into wild streptomyces fradiae. The method can also comprise: carrying out genetic engineering modification on the neomycin biosynthetic gene cluster of streptomyces fradiae to be introduced into wild streptomyces fradiae, wherein genetic engineering modification can comprise: blocking the negative regulatory gene neoI in the neomycin biosynthetic gene cluster or blocking the negative regulatory gene neoI in the neomycin biosynthetic gene cluster while driving the transcription of a key enzyme encoding gene neoE-neoD co-transcription unit by using a strong promoter. The invention also provides high-yield neomycin streptomyces fradiae obtained by the method.