34 results about "Sequence dependent" patented technology

A decision feedback structure for recovering a bit stream out of received signals is disclosed, wherein the sampling instant may be tuned in dependence of the sequence or pattern of the preceding bits so as to follow the bit sequence dependent instant of the maximum eye opening. The decision-feedback equalizer structure includes decision means for making a bit value decision at a sampling instant, a feedback path to feed back bit values to said decision means and means for adapting the sampling instant for a bit value decision made by said decision means with respect to the sampling phase depending on the bit values of preceding bits, in particular depending on the bit value of the previous bit.

The invention discloses a flexible flow shop energy consumption optimization scheduling method which has two kinds of adjustment time and is sequence dependent. The method includes the steps: (1) establishing a mathematical model in regard to minimization of total energy consumption for a flexible flow shop scheduling problem which has two kinds of adjustment time and is sequence dependent, wherein the two kinds of adjusting time include a first kind of adjusting time related to sequence and a second kind of adjusting time related to workpiece sequence and machines that workpieces are arranged to; (2) according to the established mathematical model, generating an energy consumption cost ordering strategy based on the workpiece adjusting time and processing time to schedule the workpieces so as to obtain scheduling results enabling the total energy consumption to be least.

The invention provides an intelligent contract vulnerability detection method and system, and belongs to the technical field of intelligent contracts. The method comprises the steps of uploading an intelligent contract code file; enabling the server side to execute intelligent contract vulnerability detection on the intelligent contract code file and returns an intelligent contract vulnerability detection result; wherein the smart contract vulnerability comprises a reentrant attack vulnerability; and one or more of integer value overflow vulnerability, conditional logic risk verification, access control authority risk verification, return value risk verification, random number misuse risk verification, execution sequence dependent vulnerability, timestamp dependent vulnerability, short address attack vulnerability, platform interface misuse vulnerability, denial of service attack detection vulnerability and multi-signature contract vulnerability. According to the method, the intelligent contract program file is uploaded to the server side through the client side to execute automatic detection of the vulnerability, so that manpower is liberated, detection is convenient, the speed ishigh, and the accuracy is high.

A signal-off-quantum-dot-based sensor for detecting the presence of a target molecule comprising: an aptamer probe having a nucleotide sequence which specifically interacts with the target molecule by sequence-dependent interaction, wherein the aptamer probe is sandwiched between (a) an oligonucleotide which is immobilized on the surface of a quantum dot (QD), and (b) a fluorophore-labeled oligonucleotide, wherein when the sensor is excited by an energy source: (i) in the absence of specific interaction between the target molecule and the aptamer probe, a baseline signal is emitted, and (ii) in the presence of specific interaction between the target molecule and the aptamer probe, a detection signal is emitted, wherein the baseline signal is greater than the detection signal, whereby the presence of the target molecule is detected.

The present invention provides methods and compositions for attenuating expression of a target gene in vivo. In general, the method includes administering RNAi constructs (such as small-interfering RNAs (i.e., siRNAs) that are targeted to particular mRNA sequences, or nucleic acid material that can produce siRNAs in a cell), in an amount sufficient to attenuate expression of a target gene by an RNA interference mechanism, e.g., in a sequence-dependent, PKR-independent manner. In particular, the subject method can be used to alter the growth, survival or differentiation of cells for therapeutic and cosmetic purposes.

Herein is reported a method for reducing the aggregation of an immunoglobulin in solution comprising the steps of i) comparing the amino acid sequence of the fourth framework region of the heavy chain of an antibody with a reference or germline sequence and determining whether one or more threonine residues and / or serine residues have been replaced by a different amino acid residue, and ii) modifying the amino acid sequence of the immunoglobulin by reverting the exchanged threonine residues and / or serine residues back to threonine or serine of the reference or germline sequence and thereby reducing the aggregation of an immunoglobulin in solution.

Unitary hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. One arm of the invented parallel-stem hybridization probe has a backbone polarity opposite that of the target-complementary loop sequence of the probe. Rather than interacting in an antiparallel fashion, the arms of parallel-stem hybridization probes interact in a parallel fashion. The arms of the invented dual inversion probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

This invention provides a method of sorting genes comprising: (1) preparing ds cDNA molecules from mRNA molecules (2) digesting the ds cDNA molecules (3) ligating to the digested cDNA molecules a set of dsDNA oligonucleotide adaptors; (4) amplifying the ligated cDNA molecules; and (5) sorting the amplified cDNA molecules into nonoverlapping groups. This invention also provides two additional methods of sorting genes. This invention further provides a method of making sub-libraries of ligation sets.

A method, computer program product, and computer system for generating a timing sequence for activating resources linked through time dependency relationships. A Direct Acyclic Graph (DAG) includes nodes and directed edges. Each node represents a unique resource and is a predefined Recovery Time Objective (RTO) node or an undefined RTO node. Each directed edge directly connects two nodes and represents a time delay between the two nodes. The nodes are topologically sorted to order the nodes in a dependency sequence of ordered nodes. A corrected RTO is computed for each ordered node after which an estimated RTO is calculated as a calculated RTO for each remaining undefined RTO node. The ordered nodes in the dependency sequence are reordered according to an ascending order of the corrected RTO of the ordered nodes to form a timing sequence for activating the unique resources represented by the multiple nodes.

Method of detecting a target polynucleotide based on the use of dual inversion hybridization probes having stem-and-loop structures, wherein the stem portion of the structure comprises a pair of interactive arms that are substantially prevented from interacting with target polynucleotides. The arms of the dual inversion hybridization probes interact in a conventional antiparallel fashion, but have backbone polarities opposite that of the target-complementary loop portion of the probe. Arm portions of the dual inversion probes do not substantially contribute to sequence-dependent stabilization of probe:target hybrids. Incorporating inversion linkages into the structures of these probes dramatically simplifies the process of designing stem-and-loop hybridization probes.

The invention provides a civil aviation field passenger value prediction method. According to the method, the client value of the civil aviation field is evaluated from four dimensions; Secondly, a numerical quantization model is put forward, multi-dimensional features of the RFUM model are converted into a one-dimensional numerical space through the AHP decision process, and user value changes can be analyzed from the numerical angle; And finally, proposing a sequence-dependent multi-task learning model, considering the natural correlation between the user consumption intention and the consumption amount, and predicting the future value of the user by utilizing the time sequence characteristics of the historical consumption data of the user and combining a time sequence data attention mechanism and a multi-task learning process. Implementation of the method is combined with marketing requirements in the civil aviation field, the life cycle value of the enterprise client can be predicted more accurately, and based on the prediction result, the enterprise can better formulate a client expansion strategy, the user retention rate is increased, the client value is utilized to the maximum extent, and the enterprise competitiveness is enhanced.

The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, particularly duplexes of oligonucleotides which may be used, for example, as primers or probes in PCR and / or hybridization assays. The invention also relates to methods and systems for designing and selecting oligonucleotide probes and primers having a predicted melting temperature which is optimized for such assays. To this end, algorithms and methods are provided for predicting the melting temperature of a nucleic acid having a predetermined sequence. These methods and algorithms estimate the melting temperature of a nucleic acid duplex under particular salt conditions. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of particular salt conditions on the melting temperature. As such, the methods and systems of the invention provide superior result compared to existing methods, which do not consider sequence dependent effects of changing salt conditions.

A leash held at one end by a trainer and attached at the other end to a coupler which attaches to either a harness or collar secured to the body of an animal, the coupler having therein an RF transmitter that transmits a signal consisting of one or more pulsed sequences dependent on the actuation of a switch that activates upon sensing a pulling force exerted between the animal and the trainer, said magnitude of force dependent on a spring, housed within the coupler and having an associated preset tension, wherein the RF transmitted signal is received by a receiver and a transducer located in an independent correction collar that transforms the received signal to a corresponding one or more applied electrical or mechanical pulsed forces to the bodily part of the of the animal secured by the correction collar.

A method and a system for executing a scheduled production process having an execution duration within a production schedule executed and controlled by a manufacturing execution system are disclosed, comprising the steps of: a) defining within the production process a resource required for the execution of the production process (STEP1) ; b) defining for the resource a sequence-dependent setup sub-process having a setup duration, a productive sub-process having a production duration and a sequence-dependent teardown sub-process having a teardown duration (STEP2); c) scheduling the production process for its execution with a start-time on a production schedule wherein the scheduled execution duration is the sum of the setup duration, the production duration and the teardown duration (STEP3); d) at a predetermined period prior to or at the start-time of the production process, confirming the production process for dispatching and subsequent executing (STEP4); e) in response to the confirmation of the production process for dispatching, creating individual production processes for the sequence-dependent setup sub-process, the production sub-process and the teardown sub-process, whereby the created individual production processes are linked together by precedence constraints defining a sequence of the created individual production processes (STEP5) ; f) re-scheduling the production schedule through replacing the production process by the created individual production processes (STEP6); and g) executing the re-scheduled production schedule by dispatching the created individual production in the scheduled sequence (STEP7).

A wizard is utilized when creating a server-based transactional Web page application. The wizard keeps track of the interdependencies of the Web pages, any data accesses required for any of the Web pages, and any rules for presenting a different sequence of Web pages based upon differing aspects of anticipated user input. The wizard can then be used to access the required data if a user selection is made to download the application and run the application off-line at a client that is disconnected from the network. When running off-line, the wizard plays back the downloaded pages of the application in a sequence dependent upon the tracked interdependencies and received user input. When running off-line, the wizard controls the dynamic presentation of the downloaded pages and provides the required data to enable the transactional pages of the application to be completed. The wizard, in conjunction with a browser running at the client, automatically submits the completed Web pages of the application to the server when the client is reconnected to the network.

A wizard is utilized when running a server-based transactional Web page application. The wizard tracks the interdependencies of the Web pages, any data accesses required for any of the Web pages, and any rules for presenting a different sequence of Web pages based upon differing aspects of anticipated user input. The wizard is used to access the required data if a user selection is made to download the application and run the application off-line at a client that is disconnected from the network. To run off-line, the applicable Web pages, the wizard, and the required data are downloaded to the client. When running off-line, the wizard plays back the downloaded pages of the application in a sequence dependent upon the tracked interdependencies and received user input. As such, the wizard controls the dynamic presentation of the downloaded pages and provides the required data to enable the transactional pages of the application to be completed. The wizard, in conjunction with a browser running at the client, automatically submits the completed Web pages of the application to the server when the client is reconnected to the network.

The present invention relates to methods for generating a labelled nucleic acid from an RNA comprising a 5′ protecting group, said method comprises the steps of obtaining a mixture of template strands of nucleic acids, said mixture comprising said RNA and further potentially other nucleic acids without a 5′ protecting group, annealing at least one oligonucleotide primer to the template strand of said RNA and potentially other nucleic acids, and template sequence dependent extending said primer, thereby obtaining a complementary nucleic acid strand annealed to its template strand, or providing the RNA in duplex with a complementary nucleic acid strand annealed to its template strand, andoptionally modifying the extension product of said nucleic acids without 5′ protecting group either on the 5′ end of the template strand or on the 3′ end of the complementary strand, or both, and labelling a complementary nucleic acid of a double stranded nucleic acid not modified, wherein therefore the labelled nucleic acid did have a 5′ protecting group on the RNA template strand, said 5′ protecting group being optionally removed after modification, and / or labelling a complementary nucleic acid of a double strand dependent on the presence of the 5′ protecting group on the complementary nucleic acids template RNA strand by double strand dependent ligation, thereby specifically generating a labelled nucleic acid complementary to an RNA at least originally comprising a 5′ protecting group.

Herein is reported a method for reducing the aggregation of an immunoglobulin in solution comprising the steps of i) comparing the amino acid sequence of the fourth framework region of the heavy chain of an antibody with a reference or germline sequence and determining whether one or more threonine residues and / or serine residues have been replaced by a different amino acid residue, and ii) modifying the amino acid sequence of the immunoglobulin by reverting the exchanged threonine residues and / or serine residues back to threonine or serine of the reference or germline sequence and thereby reducing the aggregation of an immunoglobulin in solution.

The invention provides a optimization of sequence dependent operations in a voice activated data packet based computer network environment. A natural language processor component can parse an input audio signal to identify a request and a trigger keyword. A prediction component can determine a thread based on the trigger keyword and the request that includes a first action, a second action subsequent to the first action, and a third action subsequent to the second action. A content selector component can select, based on the third action and the trigger keyword, a content item. An audio signalgenerator component can generate an output signal comprising the content item. An interface can transmit the output signal to cause a client computing device to drive a speaker to generate an acoustic wave corresponding to the output signal prior to occurrence of at least one of the first action and the second action.

The present invention is directed to a sequence dependent polynucleotide nanomechanical device based on a nucleic acid paranemic crossover (PX) molecule which is a four-stranded structure related to parallel double crossover molecules, except that every possible crossover takes place. Eliminating two crossovers leads to a topoisomer, termed JX2, in which one pair of ends are switched (rotated 180°) while the other pair of ends remain the same. The device can be cycled between the two states, PX and JX2, by replacing single strands that set the state to be PX or JX2.

The invention discloses a probe and a method for simultaneously detecting a plurality of microRNAs under constant-temperature conditions based on DNA assembly. The probe comprises three probe sets; the three probe sets and target microRNAs can be self-assembled into a stable 'I'-shaped structure; according to the high specificity of restriction endonucleases and the cutting property of the same tosingle-stranded DNAs, DNA modules with non-sequence-dependent nuclease functions are realized, and are applied to simultaneous fluorescence detection of the miRNAs under the constant-temperature conditions. The probe and the method have the advantages that the system complication of conventional constant-temperature amplification methods is solved, and a new method is provided for simultaneous sensitive detection of the miRNAs; only the restriction endonucleases and the simply-designed probe are needed, and the constant-temperature signal amplification detection of nucleic acids can be realized without addition of dNTPs; the probe has high precision, repeatability and stability, and is suitably developed into a kit and popularized to the market.

Systems and methods to combine multiple voice activated audio input data packets that indicate sequence dependent operations are provided. A natural language processor component can receive first andsecond input audio signal from a client computing device, and can identify respective requests and corresponding trigger keywords. A direct action application programming interface ("API") can generate respective action data structures, and can construct respective data transmissions including the respective action data structures. A thread optimization component can obtain data packets of the first data transmission, and can obtain data packets of the second data transmission. The thread optimization component can determine, based on a heuristic technique applied to the data packets of the respective data transmissions a sequence dependency parameter. The thread optimization component can merge, based on a comparison of the sequence dependency parameter with a threshold, the first and second data transmissions into a single thread.

The execution of a dynamic code segment sequence that includes at least two code segments in sequence. The first code segment is first executed and the corresponding state of the environment is captured. The second code segment is then executed. When the second code segment is later re-executed, the first code segment is not re-executed again. Rather, the environmental state is set to be the captured state that existed when the first code segment originally executed. Then, the second code segment may be executed without spending the resources required to re-run the first code segment. This may be employed at authoring time, or after deployment time.

A signal-off-quantum-dot-based sensor for detecting the presence of a target molecule comprising: an aptamer probe having a nucleotide sequence which specifically interacts with the target molecule by sequence-dependent interaction, wherein the aptamer probe is sandwiched between (a) an oligonucleotide which is immobilized on the surface of a quantum dot (QD), and (b) a fluorophore-labeled oligonucleotide, wherein when the sensor is excited by an energy source: (i) in the absence of specific interaction between the target molecule and the aptamer probe, a baseline signal is emitted, and (ii) in the presence of specific interaction between the target molecule and the aptamer probe, a detection signal is emitted, wherein the baseline signal is greater than the detection signal, whereby the presence of the target molecule is detected.

Reduction of energization transients in a three phase Y-connected load having legs A, B and C associated with corresponding phases of a three-phase AC power system is achieved by energizing respective legs of the load in an order determined by a energization sequence dependent upon a type of the load. The energization sequence defines first, second and third legs of the legs A, B and C to be energized. A conduction path for current to flow between a neutral point of the Y-connected load to ground through a transient-limiting impedance while the first, second and third legs are being energized is employed. The transient limiting impedance has an impedance value selected such that after the first leg is energized, an energization transient produced on energizing the second leg and an energization transient produced on energizing the third leg are both approximately minimized.

Systems and methods of handling sequence dependent operations. A natural language processor component can parse an input audio signal to identify request and trigger keywords. A prediction component can determine a thread based on the trigger keyword and the request, including a first action, a second action subsequent to the first action, and a third action subsequent to the second action. A content selector component can select a content item based on the third action and the trigger keyword. An audio signal generator component can generate an output signal including the content item. Prior to occurrence of at least one of the first action and the second action, the interface can transmit the output signal to cause the client computing device to drive the speaker to generate an acoustic wave corresponding to the output signal.

The present invention provides methods and compositions for attenuating expression of a target gene in vivo. In general, the method includes administering RNAi constructs (such as small-interfering RNAs (i.e., siRNAs) that are targeted to particular mRNA sequences, or nucleic acid material that can produce siRNAs in a cell), in an amount sufficient to attenuate expression of a target gene by an RNA interference mechanism, e.g., in a sequence-dependent, PKR-independent manner. In particular, the subject method can be used to alter the growth, survival or differentiation of cells for therapeutic and cosmetic purposes.

A method of obtaining fluorochromes that bind to specific sites on proteins is presented. The method consists of synthesizing a peptide bearing a sequence from a parent protein and a second peptide of similar composition but with a scrambled sequence. Each peptide is then added to a test fluorochrome at similar concentrations, and a difference in fluorescence is obtained. A difference in fluorescence indicates a sequence dependent interaction between the peptide and fluorochrome. A large number of fluorochromes can be screened using the two peptides to find one binding in a sequence dependent fashion. This fluorochrome can then be tested for binding to the original full-length protein. The fluorochrome can then serve as scaffold in medicinal chemistry. The fluorochrome / peptide interaction and resulting fluorescence can be inhibited by non-fluorescent test compounds, which are compounds that bind to this peptide. Thus. the peptide / fluorochrome interaction can provide assays screening for therapeutic drugs binding to this site on the protein.

A system for recognizing human activity from a video stream includes a classifier for classifying an image frame of the video steam in one or more classes and generating a class probability vector for the image frame based on the classification. The system further includes a data filtering and binarization module for filtering and binarizing each probability value of the class probability vector based on a pre-defined probability threshold value. The system furthermore includes a compressed word composition module for determining one or more transitions of one or more classes in consecutive image frames of the video stream and generating a sequence of compressed words based on the deter-mined one or more transitions. The system furthermore includes a sequence dependent classifier for extracting one or more user actions by analyzing the sequence of compressed words to and recognizing human activity therefrom.