152 results about "New genetics" patented technology

The invention relates to a resource allocation global optimization method of an intelligent scheduling system. The resource allocation global optimization method is implemented by adopting a new genetic algorithm. The resource allocation global optimization method considers the difference between a full-load driving path and a no-load driving path of an AGV, adds a conversion coefficient alpha before the no-load driving path, the value of the conversion coefficient alpha ranges from 0 to 1, and establishes a new mathematical model for AGV global optimization scheduling f=min max{d1,d2,...,dn}.Based on the improvement of a crossover operator, a best-worst crossover mode is proposed on the basis of a hybrid crossover mode combining two-point crossover and BCBRC, thus the problem that a non-feasible solution is prone to generate due to changes of chromosome coding rules is avoided; and a variation pattern of gene segment random exchange is adopted, thereby avoiding the problem that the conventional variation mode is prone to generate a non-feasible solution containing the same nodes when a real number system coding method is adopted.

The invention discloses a pear transcription factor PyGSTf12, and a recombinant expression vector and application thereof. The sequence difference between the PyGSTf12 and a promoter thereof is used as a molecular marker for identifying red and green peels, wherein the PyGSTf12 and the promoter are respectively separated from 'Red Zaosu' pears and yellow bud mutated fruits thereof, and the nucleotide sequences of the PyGSTf12 and the promoter are respectively as shown in SEQ ID No. 31 and SEQ ID No. 32. By a dual-luciferase report system, an agrobacterium-mediated instant genetic transformation method proves that the PyGSTf12 co-transformed by transcription factors PyHY5 and PyMYB114 promotes accumulation of anthocyanin in strawberry and pear fruits, and the PyGSTf12 promoter promotes thetransport function of the anthocyanin; through discovery of the PyGSTf12 gene, new genetic resources are provided for molecular breeding that promotes synthesis of the anthocyanin in pear peels, and new genetic resources are provided for the implementation of green agriculture; the development and utilization of the genetic resources is beneficial to agricultural cost reduction and environmental friendliness.

The invention mainly relates to a culture medium composition suitable for germ-free germination of orchid seeds and a method thereof. Various components of a basic culture medium and a seed germination culture medium are as follows: 500mg / L of calcium nitrate, 200mg / L of potassium chloride, 20-30g / L of sucrose or white sugar and 5-7g / L of agar, wherein the basic culture medium is selected from self-improved MS, i.e. on the basis of the original MS culture medium, 440mg / L of calcium chloride in the MS basic culture medium is replaced into 500mg / L of calcium nitrate and 200mg / L of potassium chloride; and the pH value is 5.0-5.6. The seed germination culture medium comprises the basic culture medium, 0.5-1.0mg / L of 6-BA, 0.5-1.0mg / L of NAA, 1.0g / l of active carbon and 50-100m / L of coconut milk. The invention has the advantages that: (1) the orchid group-cultured optimized culture medium has strong pertinence, good adaptability and high seed germination rate; (2) the method greatly shortens the breeding period of orchid so as to rapidly obtain new genetic improvement; and (3) the method is convenient for operation and reduces the pollution to the minimum extent.

The invention discloses a pear transcription factor PyHY5 and application of a recombinant expression vector of the pear transcription factor PyHY5. The nucleotide sequence of a transcription factor PyHY5 gene which is separated from 'Red Zaosu' pears and has a function of promoting anthocyanin biosynthesis of pear peels is as shown in SEQ ID No. 1, and the encoded amino acid sequence of the transcription factor PyHY5 gene is as shown in SEQ ID No. 2. Transcription factors PyHY5 and PyMYB114 are co-transformed into strawberry and pear fruits by an agrobacterium-mediated transformation method;according to biological function verification, the cloned PyHY5 gene and a cofactor PyMYB114 thereof interact to promote the transport function of anthocyanin in the pear peels; through discovery of new functions of the PyHY5, new genetic resources are provided for molecular breeding that promotes accumulation of the anthocyanin in the pear peels, and new genetic resources are provided for the implementation of green agriculture; the development and utilization of the genetic resources is beneficial to agricultural cost reduction and environmental friendliness.

The invention belongs to the technical field of rape molecular breeding and preparation of molecular markers, and particularly relates to preparation of a brassica napus L. grain weight major QTLs locus specific molecular marker. The marker can be used for auxiliary selection of molecular markers, fine positioning of grain weight trait loci and based cloning in the improvement on brassica napus L. grain weight traits. The marker is characterized in that double haploid (DH) is constructed by hybridizing brassica napus L. first A254 (large-grain pure line materials) as a female parent and brassica napus L. first A177 (small-grain pure line materials) as a male parent, and the molecular marker which is tightly linked with the grain weight major QTLs is obtained by analyzing DH group genotypes and thousand grain weight data and is named I0509 and J0609, and is relatively verified and applied. A new genetic marker is provided for molecular breeding of rape grain weight and also useful information is provided for fine positioning of thousand grain weight trait loci of the brassica napus L. and based cloning of relative genes.

This disclosure relates to methods and systems for a curated genetic variant database and systems and methods for submitting new genetic tests based on the information in the curated database. The methods and systems of the invention further provide for a single curated variant database that allows curation of genetic variants while protecting the proprietary nature of the information submitted to the database. The system and methods also provide for submission of new genetic tests based on genetic variants, conducting genetic tests, and for determining payments to submitters and test developers based on the genetic tests.

The invention provides new genetic engineering bacteria used for producing beta-alanine, having strong synthetic ability and having higher enzyme activity, construction, expression and purification of the high-production engineering bacteria, and methods for synthesis of beta-alanine respectively through whole cell transformation and fermentation liquid direct transformation. A synthetic method comprises the steps: an L-aspartic-alpha-alanine decarboxylase (PanD) gene of lactobacillus plantarum is obtained by a gene engineering method and has the gene sequence number of NC-004567.2, the gene is constructed into a high-efficiency expression vector, then the high-efficiency expression vector is transformed into recipient bacteria, and thus the genetic engineering bacteria for producing beta-alanine are obtained. The genetic engineering bacteria are subjected to fermentation culture, 80 g / L of a substrate can be transformed by whole cells, and the beta-alanine content reaches 59.7 g / L; and the substrate is directly added into a fermentation liquid, the transformation concentration can reach 10 g / L, the beta-alanine content reaches 6.8 g / L, and the synthetic ability is higher than that of conventional reported genetic engineering bacteria.

The invention belongs to the field of rape molecular breeding, and relates to a specific molecular marker of brassica napus L. grain weight and a preparation method and an application. Hybridization is performed by using brassica napus L. A254 as a female parent and using brassica napus L. A177 as a male parent to construct a dihaploid colony (DH); data of the genotype and 1000-grain weight of the DH colony are analyzed to obtain QTLs loci of the grain weight character. MINI3 and TTG2 genes of A254 and A177 are cloned by a homologous sequencing method; specific molecular marker MINI3a and TTG2a of the MINI3 and TTG2 genes are designed according to sequence different loci; the molecular marker MINI3a and TTG2a are positioned on two grain weight QTLs loci of a A5 linkage group; related verification and application demonstrate that the molecular marker prepared by the invention is a new genetic marker. The invention provides a new genetic marker for rape grain weight molecular breeding.

Provided are a method and an apparatus for deciding a channel quality indicator (CQI) in a wireless communication system. The method includes randomly generating CQI values encoded into genotypes to form an initial genetic group; evaluating fitnesses using the CQI values and a measured block error rate (BLER), and if the CQI value indicating the highest fitness is not within a range of the BLER, selecting a specific gene of genes of the initial genetic group to perform crossover and mutation operations; and repeating the crossover and mutation operations to allow a new genetic group generated by the crossover and mutation operations to be within the range of the BLER.

The invention discloses a cold-resist gene PtrERF109 of Poncirus trifoliata and application thereof in cold resistance genetic improvement of plants. The PtrERF109 gene is a transcription factor isolated and cloned from extremely-cold-resist Poncirus trifoliata, and the sequence is as shown in SEQ. ID NO. 1. Overexpression RNAi vectors of the gene are constructed, and the vectors of the PtrERF109gene are respectively introduced into tobacco, lemon and Poncirus trifoliata by Agrobacterium-mediated genetic transformation to obtain transgenic plants. Verification of biological functions of the obtained transgenic plants indicates that the cloned PtrERF109 gene has the function of improving the cold resistance of the plants. The discovery of the gene provides new genetic resources for anti-reverse molecular design and breeding of the plants, and provides new genetic resources for the implementation of green agriculture and water-saving agriculture. The development and utilization of the genetic resources are conducive to reducing agricultural production costs and achieving environmental friendliness.

This disclosure provides new genetic targets, diagnostic methods, and therapeutic treatment regimens for multiple autoimmune disorders, including pediatric autoimmune disorders that are co-inherited and genetically shared. The disclosure, for example, provides methods of diagnosing or determining a susceptibility for one or more autoimmune diseases and methods of determining treatment protocols for patients with one or more autoimmune diseases based on determining if the patients have genetic alterations in particular genes.

Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

The invention belongs to the field of plant genetic engineering, and particularly relates to a poncirustrifoliata mitogen-activated protein kinase (PtrMAPK) gene which is obtained from poncirustrifoliata by separation and cloning. The nucleotide sequence of the gene is shown as a sequence table SEQ ID NO:1; the corresponding amino acid sequence of the gene is shown as a sequence table SEQ ID NO:2; and the gene comprises 1128bp of open reading frame, and encodes 375 amino acids, an isoelectric point is 5.51, and predicted molecular weight is 43kD. The PtrMAPK gene is imported into tobacco for functional verification, and the drought resistance capacity of the obtained transgenic tobacco plant can be improved obviously. New gene resources are provided for anti-abiotic stress molecular design and breeding, new genetic resources are provided for implementing green and environment-friendly agriculture, and the development and utilization of the genetic resources contribute to reducing agriculture production cost and achieving environment-friendliness.

This invention proposes a detection method of haplotype comprised of polymophic loci (rs3138774) in (TA)n, rs2077647 in T29C, rs2234693 in Pvu II and (TA)n, T29C, Pvu II and Xba I in susceptibility gene ESR1 relates to liver cancer risk. The methods are PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism), fragment analysis and direct sequencing PCR products. The haplotype is constructed by PHASE program. The invention also relates to the kits detecting susceptibility polymophic locis and using methods. In addition, site(TA)n (rs3138774), T29C(rs2077647) , Pvu II(rs2234693) and the haplotype comprising of (TA)n, T29C, Pvu II and Xba I were related to liver cancer risk. ESR1 is determined as a new susceptibility gene of liver cancer, which provides new genetic consultation content for the liver cancer prevention and control.

Presented herein are novel protein biomarkers related to cancers with stromal components. These newly identified biomarkers create the basis for multiple (single) assays using traditional bioassay technologies and when used in combination yield exceptional clinical sensitivity and specificity in the determination of diagnosis and / or prognosis of cancer. A new genetic model able to identify potential genetic suppressors and / or potential therapeutic agents for treating stromal cancers is also described. Means and methods for evaluating data generated using multiple biomarkers in order to validate findings and further the use of the biomarkers and the genetic model system in clinical, diagnostic and therapeutic uses is also included.

The invention belongs to the field of plant genetic engineering, and discloses a poncirus trifoliata cold resistant gene PtrTZF1 and an application thereof to plant cold resistant heredity improvement. The PtrTZF1 gene is C3H type zinc finger protein separated and cloned from cold resistant poncirus trifoliata, and the amino acid sequence of the PtrTZF1 gene is shown as SEQID NO.2. The gene is subjected to construction of overexpression and an RNAi carrier, through agrobacterium tumefaciens mediated heredity transformation, the gene is guided into tobacco, lemons and poncirus trifoliate respectively, through biological function verification, the obtained genetic modification plant can indicate that the cloned PtrTZF1 gene has the function of improving cold resistance of plants. The gene isfound so that a new genetic resource is provided for designing and breeding of plant inverse resisting molecules, and a new heredity resource is provided for implementation of green agriculture and water-saving agriculture. The development and the utilization of the heredity resource are favorable for reduction of agricultural production cost and realization of purpose of being environmental-friendly.

The invention discloses a cabbage type rape grain weight-associated molecular marker and a preparation method and an application thereof. The preparation of the molecular marker comprises the following steps: 1) by the utilization of 91 unit points of SSR markers uniformly distributed on an oilseed rape genetic map, 576 varieties of oilseed rape from all around the world undergo genotype and population structure analysis and association analysis is carried out with the combination of thousand grain weight data character data; 2) 14 high-quality SSR markers are developed near the marker BrSF7-181 by the utilization of cabbage and oilseed rape whole genome sequence information so as to carry out association analysis once again, wherein BrSF6-2025 has the highest significance level; and 3) by the utilization of the molecular marker, grain weight extreme varieties in genetic resource populations are verified. The invention provides a new genetic marker for molecular breeding of rape grain weight and also provides useful information for map-based cloning of cabbage type rape grain weight genetic locus.

The invention discloses an MDM2 gene which is a novel predisposing gene of nasopharyngeal darcinoma by relating the SNP 309 polymorphic loci of the MDM2 gene with the risk of the nasopharyngeal darcinoma and the more serious lymph node metastasis, thereby providing new genetic counseling contents and the molecule criterion for people to prevent and control the nasopharyngeal darcinoma. In addition, the invention also relates to a method for detecting the SNP309 polymorphic loci and the corresponding detecting kit, wherein, the detecting method is the PCR product direct sequencing method that is the polymerase chain reaction-sequencing method.

The invention discloses a rice blast-resistant locus Pi-jx and Indel marker primers and breeding application thereof. According to the invention, with a natural variety as a locating population, a newrice blast-resistant genetic locus Pi-jx is located on a twelfth chromosome of rice by a genome-wide association analysis method. On such basis, the reliability of the Pi-jx locus is further verifiedin an F2 segregating population of a resistant / susceptible variety by a genomic hybrid pool Mutmap method. Finally, polymorphic Indel molecular markers are designed for genome sequences near the resistant locus, and the Pi-jx is introduced into the susceptible variety through molecular marker-assisted selection, so that the rice blast resistance of receptor parents is improved. The locating and the breeding application of the Pi-jxresistant locus provide a new genetic resource for breeding a broad-spectrum rice blast-resistant variety.

The invention belongs to the technical field of the preparation of livestock molecular genetic markers and particularly relates to a genetic marker for a porcine litter size character and an application of the genetic marker. A T base insertion / deletion mutation of a tenth intron tail end sequence of a porcine STIM1 gene is taken as a genetic marker related to the porcine litter size character. According to the genetic marker provided by the invention, the genetic marker related to the porcine litter size character is obtained through preparation, wherein a nucleotide sequence of the genetic marker of a Yorkshire pig is as shown in SEQ ID NO:1, and a T base insertion mutation exists at 256bp of the sequence; a nucleotide sequence of the genetic marker of a Meishan pig is as shown in SEQ ID NO:2, and a T base deletion mutation exists at 256bp of the sequence; and the mutations can obviously influence the porcine litter size character. The invention further discloses a preparation method for the genetic marker and an application of the genetic marker to the association analysis of the porcine litter size character. A new genetic marker is provided for the molecular marker auxiliary breeding of the porcine litter size character.

The invention relates to a detection method, kit and application thereof for the polymorphic site rs17401966 in the susceptibility genome region 1p36.22 related to the risk of HBV-related hepatocellular carcinoma (Hepatocellular carcinoma, HCC). The method is SNPstream typing and TaqMan typing, and the present invention further relates to a kit for detecting the above-mentioned susceptible sites and its application. In addition, the present invention associates the polymorphic site rs17401966 in the 1p36.22 region with the risk of HBV-related HCC, and determines that 1p36.22 is a new susceptibility gene region of HBV-related HCC, which is the key factor for HBV-related HCC. Population prevention and control provide new genetic counseling content.

The invention discloses a cucumber CsDIR16 gene and application of the cucumber CsDIR16 gene in lowering plant propamocarb hydrochloride pesticide residues. A nucleotide sequence of the cucumber CsDIR16 gene is shown as SEQ ID NO:1, the total length of the CsDIR16 is 588bp, an amino acid sequence of protein encoded by the CsDIR16 is shown as SEQ ID NO:2, 195 amino acids are encoded, and the CsDIR16 belongs to a Dirigent like protein family. An agrobacterium tumefaciens-mediated genetic transformation method is utilized to transform cucumbers, and an obtained transgenic plant is subjected to biological function verification, shown that the cloned CsDIR16 gene of the invention or the protein encoded by the CsDIR16 gene has the function of lowering the plant propamocarb hydrochloride pesticide residues, so that investment of a pesticide is reduced, the agricultural production cost is lowered, efficient development of agricultural industry is facilitated, and a new genetic resource is supplied to implement green agriculture and organic agriculture.

The invention discloses a functional marker associated with drought resistance of rape in the seedling stage and application of the functional marker. The sequence of the functional marker is as shown in SEQ ID NO:1, and the molecular weight is 465 bp. Application of the functional marker in drought-resistant seedling breeding of rape includes the steps that firstly, the DNA of a rape plant is extracted; secondly, PCR amplification is conducted, wherein the primer sequence F is 5'-TGAGGCTGATAGGATACTGG-3', and the primer sequence R is 5'-CCACGGGCACCTACATTA-3'; thirdly, after the amplification product is subjected to electrophoretic separation with agarose gel with the concentration being 1.5%, it is predicted that draught resistance of the rape plant is good when a specific band of the functional marker with the molecular weight being 465 bp occurs, and it is predicted that draught resistance of the rape plant is poor when the specific band of the functional marker with the molecular weight being 465 bp lacks. The new genetic marker is provided for genetic improvement of drought resistance of the rape in the seedling stage, the problems that a conventional seedling breeding method is large in drought resistance identification workload, the period is long and the method is influenced by the environment easily are solved, selection efficiency of drought resistance seedling breeding of rape is remarkably improved, and the seedling breeding progress of new varieties is accelerated.

The invention discloses a wheat TaRPP13 gene and application thereof in wheat powdery mildew resistant breeding. cDNA of a gene TaRPP13 of powdery mildew induced response in cultivating wheat Brock is3232 bp in total length, 5'UTR is 104 bp in length and 3'UTR is 194 bp in length, and an open reading frame is 2934 bp. A polypeptide chain of 977aa is coded, and has the homology of 93%, 79% and 66%with RPP13 gene-coded amino acid sequences in urartu wheat, brachypodium sylvaticum and corn respectively. The polypeptide chain has coiled-coil(CC), NB-ARC and LRR protein structural domains. Underthe stress of powdery mildew, a difference of expression of the gene in disease resistant wheat and disease infected wheat is apparent in time and expression quantity. Disease resistance of the disease resistant wheat Brock is reduced because of TaRPP13 gene silencing. The gene is capable of providing a new genetic resource for a wheat powdery mildew resistant mechanism and disease resistant breeding.

The invention relates to an automobile operation condition design method based on prior information and big data. The method comprises the three stages that first, feature parameters expressing representativeness are determined; second, a typical condition database and a resampling database are used as prior condition databases, and states and state transfer information of the prior condition databases are subjected to statistical analysis to serve as prior information for condition design; and third, a new genetic algorithm based on the prior information is utilized to design conditions. Themethod is a new method which utilizes the big data with low sampling frequency to design the automobile operation conditions, and the problem that a current automobile operation condition design method is invalid to data with low sampling frequency lower than 1Hz is effectively solved.

The invention provides genetic engineering bacteria for catalyzing citral to generate citronellal and a construction method thereof. Recombinant expression plasmid provided by the invention is to clone gene gye with NADH oxidase on a suitable vector. The genetic engineering bacteria provided by the invention is transformed into the recombinant expression plasmid in escherichia coli. The invention provides new genetic engineering bacteria for producing citronellal, catalyzes hydrogenation of citral selectively to generate citronellal by employing the expression product NADH oxidase of the gene gye of NADH oxidase, which simplifies manufacturing technique and improves production efficiency. The invention sets a precedent of utilizing genetic engineering bacteria to produce citronellal, as a result, the normal escherichia coli is provided with the function of catalyzing citral to generate citronellal, which provides a new thinking for constructing the genetic engineering bacteria for produce citronellal and lays a foundation for achieving a more optimal engineering bacteria.

The invention discloses a pear lignin synthesis gene PbMC1a / 1b and application of the pear lignin synthesis gene PbMC1a / 1b in genetic improvement of fruit quality. The PbMC1a / 1b gene is a cysteine-like protease isolated and cloned from Dangshansu pear with high lignin content, an applicant names the gene as PbMC1a / 1b, the sequence is shown as SEQ ID NO.1, and the corresponding amino acid sequenceis shown as SEQ ID NO.2. A gene construction over-expression vector is introduced into arabidopsis thaliana, obtained transgenic plants are verified by a biological function, the cloned PbMC1a / 1b genecan promote the cell walls of fibers between ducts, lignocellulosic fibers and vascular bundles to be obviously thickened, lignin accumulation in stems is promoted and plant growth is inhibited, theexpression of genes related to lignin biosynthesis is increased, new genetic resources is provided for molecular design and breeding of fruit quality, and the PbMC1a / 1b gene is an important candidategene for future genetic engineering to improve fruit quality breeding.

The invention discloses a pear transcription factor PbrMYB169 and an application thereof. The pear transcription factor PbrMYB169 is separated from Dangshan crisp pears, and has MYB family member PbrMYB169 genes capable of adjusting and controlling development of sclereid of pear fruits, the nucleotide sequence of the pear transcription factor PbrMYB169 is as shown in SEQID No.1, and the coding amino acid sequence of the pear transcription factor PbrMYB169 is as shown in a sequence table SEQID No.2. Through an agrobacterium tumefaciens mediated heredity conversion method, PbrMYB169 transcription factors are converted into arabidopsis thaliana, and transgenic plants are obtained; biological function verification indicates that the PbrMYB169 gene cloned through the pear transcription factorPbrMYB169 has the function of promoting synthesis of arabidopsis thaliana inflorescence stem lignin, besides, the PbrMYB169 transcription factor has the advantage of being capable of adjusting and controlling a plurality of genes, and an efficient way is provided for molecular breeding. New genetic resources are provided for reduction of molecular breeding of synthesis of organism lignin, new heredity resources are provided for implementing green agriculture, and reduction of the agriculture cost and realization of an environmental-friendly purpose can be facilitated through development and utilization of the heredity resources.

The invention relates to a DNA molecular weight standard with an even 200 bp gradient and a rapid preparation method thereof. A super plasmid is constructed by a brand-new genetic engineering method; the DNA molecular weight standard with the even 200 bp gradient is prepared by methods of bacillus coli fermentation and restricted enzyme digestion and is a restricted enzyme digestion mixture comprising seven DNA bands with the lengths of 400 bp, 600 bp, 800 bp, 1000 bp, 14000 bp, 2000 bp and 3000 bp, and the densities (masses) of the seven DNA bands have a certain proportional relation of 2:3:4:5:7:10:15. The rapid preparation method of the DNA molecular weight standard with the even 200 bp gradient comprises the following steps: enabling the seven DNA bands to use a plasmid pYE 9600 as an enzyme digestion object, extracting a target plasmid pYE9600 by methods of bacillus coli fermentation and alkaline lysis, purifying the target plasmid pYE9600 and using a restricted enzyme EcoRI for enzyme digestion so as to release 200 bp serial sections contained in the target plasmid pYE9600.