45 results about "Locus (genetics)" patented technology

The invention relates to rapid identification of anti-powdery-mildew gene of Brachypodium distachyon, relates to the knowledge of plant comparative genomics, genetics, biological information and other subjects, and belongs to the scientific field of plant biotechnology. The rapid identification of anti-powdery-mildew gene of Brachypodium distachyon comprises the main steps of: (1) downloading full genome sequences of Brachypodium distachyon and collecting MLO (mildew resistance locus o) type gene; (2) identifying the MLO type gene; (3) analyzing the MLO type gene historical development relation; and (4) comparing the MLO type powdery mildew gene. According to the rapid identification of anti-powdery-mildew gene of Brachypodium distachyon provided by the invention, the excavation period of the anti-powdery-mildew gene of Brachypodium distachyon is effectively shortened, and the rapid identification of the powdery mildew gene is facilitated; the identified powdery mildew gene can be used for developing corresponding coseparation functional markers (SNP (single nucleotide polymorphism), SCAR (sequence-characterized amplified region), and the like), and can also be rapidly used for assisted selection of molecule markers of anti-powdery-mildew gene, and the accuracy is high; development of multi-resistance breeding materials can be carried out by combining with other anti-disease gene molecule markers, the breeding time is shortened and the breeding efficiency is improved; and foundation is established for expounding the molecular mechanism of the anti-powdery-mildew gene of Brachypodium distachyon.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker influencing the wool shearing amount of alpine merino and application. The SNP molecular marker is located at the 20065540th nucleotide site G / A mutation on the fifth chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the shearing amount of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a high-mountain merino sheep shearing amount early selection technology is established, the breeding time of the excellent character of the shearing amount of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP molecular marker influencing weaning weight character of merinos and application. The SNP molecular marker is located at the 3566348 nucleotide site T / C mutation on the 23<rd> chromosome of the international sheep reference genome Oar_v4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair, and a nucleotide polymorphism detection method. Compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight characters of high-mountain merinos, shortening the breeding period and accelerating the breeding process, an early selection technology of the weaning weight characters of the high-mountain merinos is established, the breeding time of the excellent weaning weight characters of the high-mountain merinos is shortened, the breeding cost is reduced, and the application value is very high.

The invention relates to a composite amplification system of 23 short tandem repeat sequences and a kit, is used for detecting heredity mark gene of polymorphism in a mankind genome, and belongs to the biology technical field. The invention relates to a scheme that a plurality of short tandem repeat sequences are simultaneously amplified in a PCR system; a specific gene locus comprises 22 short tandem repeat sequences with high level heredity polymorphism and 1 gender determination locus; primers are respectively designed and a fluorophore is marked; the system has very high individual recognition rate and non-father elimination rate; the system and kit can be used for legal medical individual recognition, paternity tests, and colony genetics analysis, is high in accuracy, and good in sensitivity.

The invention relates to a gene locus closely associated with the level of milk polyunsaturated fatty acid and application thereof, belonging to the technical field of genetics. The invention provides a gene locus closely associated with the level of milk polyunsaturated fatty acid, namely a fatty acid desaturase 1 gene (FADS1) locus, i.e. an FADS1 locus having rs174556 mononucleotide polymorphism. The mononucleotide polymorphism rs174556 base C of the locus is mutated into T, thus being closely associated with the level of milk polyunsaturated fatty acid. The levels of arachidonic acid and eicosapentaenoic acid in milk of a wet nurse carrying FADS1 gene rs174556T / T minor allele homozygote are obviously degraded, and the locus has mononucleotide polymorphism determining the level of milk polyunsaturated fatty acid. The FADS1 locus and a coding protein thereof are of great importance in elucidating a polyunsaturated fatty acid metabolic mechanism, establishing individual nutrition intervention and establishing appropriate dietary recommended intakes of polyunsaturated fatty acid based on different genetic backgrounds.

The invention discloses a molecular marking method for simultaneously predicting abdominal fat characters, leg skeleton characters and reproductive performance of broilers and an application, and belongs to the technical field of animal molecular genetics. The invention discloses a method for identifying low-fat broilers more accurately and conveniently and accelerating broiler breeding process. According to the invention, primers are designed according to the sequences from the 208 bp at the upstream to the 72 bp at the downstream of the SNP locus g.3656C > T of the chicken SREBP1 gene exon 3to obtain an amplification primer, then carrying out PCR amplification on the chicken genomic DNA according to the obtained primer, and carrying out enzyme digestion on the amplification product by using restriction endonuclease to obtain the chicken marker genotype. The method is simple to operate, low in cost and high in accuracy, can be used for automatic detection, is an effective molecular marker breeding means, and can be used for accelerating the breeding process of chickens and cultivating low-fat broilers with healthy leg bones and good reproductive performance, which meet market requirements, so that good economic benefits are obtained.

The invention relates to the field of molecular genetics, discloses a molecular marker of a pear PpAIV2 gene locus and further discloses a screening method of the molecular marker of the pear PpAIV2 gene locus. A genome simple sequence repeat (SSR) locus adjacent to pear soluble acid invertase gene PpAIV2 is predicated and a locus specific primer is designed; one SSR allelic variation locus which is tightly interlocked with the PpAIV2 is obtained through amplification in germplasms with different genetic backgrounds and F1-generation population genomes; the SSR allelic variation locus is named as P.zaas-1. The pear soluble acid invertase gene PpAIV2 is a key gene for accumulating pear fruit sucrose; the P.zaas-1 has potential identification and screening effects on materials with remarkable differences on accumulation of the fruit sucrose.

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker for identifying the weaning weight of alpine merino and application of the SNP marker, and the SNP molecular marker is located at the 3566034th nucleotide site C / T mutation on the 23rd chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the weaning weight of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a technology for early selection of the weaning weight of the high-mountain merino sheep is established, the breeding time of excellent characters of the weaning weight of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

The invention provides a functional gene molecular marker of a rice blast resistance locus Pi2 / 9 and application thereof. The molecular marker of a specific band type for rice blast resistance genes Pi2, Piz-t, Pi9 and Pigm is amplified from rice genome DNA by virtue of a primer pair SEQ ID NO.1-2. The functional gene specific molecular marker of the rice blast resistance locus Pi2 / 9 has importantapplication value, and utilization efficiency of the gene in germplasm resource screening, molecular marker-assisted selective breeding, gene pyramiding breeding and transgenic breeding can be increased by utilizing the marker.

The invention provides a fluorescently-labeled X-InDel locus composite amplification system. By means of the system, eighteen insertion-deletion genetic polymorphism loci on an X chromosome can be compositely amplified, wherein the eighteen loci are respectively labeled with three fluoresceins FAM, HEX and TAMRA. The composite amplification system is high in sensitivity and individual identification rate, is high in polymorphism, is good in stability and repeatability, is accurate in typing results and can satisfy a practical requirement. A kit can be manufactured on the basis of the composite amplification system, can be used for paternity test, dyad paternity test, grandparent and grandchild test, sibling test and individual identification. New technologies can be provided for the fields such as anthropology, medical genetics and the like.

The invention belongs to the technical field of biological genetics, and in particular, relates to a corn sucrose synthase mutation gene, a protein encoded by the gene, and an application of the gene. The corn sucrose synthase mutant sh1-m comprises a nucleotide sequence shown in SEQ ID No:1. The protein encoded by the corn sucrose synthase mutant sh1-m comprises an amino acid sequence shown in SEQ ID No:2. The sh1-m mutant is discovered in an improved Zheng58 selfing line material (Gai-Z58), a gene sequence structure is verified, and the corn sucrose synthase mutant sh1-m is confirmed to be an allele of Sh1 gene locus, can significantly improve the endosperm amylose content (16.5%), and has excellent application value.

The invention discloses an RFLP method for fast detecting FLII genes of cattle and application thereof. The method includes the steps that with cattle genome DNA as a template and a primer pair P as primers, PCR amplification is performed on an intron 22 area of the FLII genes of the cattle, PCR amplification products are digested through restriction enzyme Sac II, then agarose gel electrophoresis is performed on the enzyme-digested segments, the single-nucleotide polymorphism of the intron 22 area of the FLII genes of the cattle is identified according to an agarose gel electrophoresis result, an SNP locus is remarkably relevant with the growth and development character of the cattle and can serve as a molecular genetics marker closely relevant to the growth character of the cattle in aspects of DNA level screening and detection, the RFLP method is applied to auxiliary selection of marking of the meat type growth character of the cattle, and therefore a cattle population good in genetic resource can be fast established.