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54 results about "Lactate concentration" patented technology
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In health, blood lactate concentration is maintained within the approximate range of 0.5-1.5 mmol/L [6]. This reflects a balance between the rate of lactate release to blood from erythrocytes and other tissue cells and rate of lactate clearance from blood, principally by the liver and kidney.
Smart disposable plastic lab-on-a-chip for point-of-care testing
InactiveUS20050130292A1None of measures has been particularly successfulRelieve painBioreactor/fermenter combinationsCombination devicesVenous bloodLab-on-a-chip
Disclosed herein is a fully-integrated, disposable biochip for point-of-care testing of clinically relevant parameters. Specifically, in accordance with an embodiment of the present invention, the biochip is designed for POCT (point-of-care-testing) of an array of metabolic parameters including partial pressure of oxygen, Glucose, and Lactate concentration from venous blood samples. The biochip is fabricated on a low-cost plastic substrate using mass manufacturing compatible fabrication processes. Furthermore, the biochip contains a fully-integrated metallic micro-needle for blood sampling. The biochip also uses smart passive microfluidics in conjunction with low-power functional on-chip pressure generators for microfluidic sequencing. The design, configuration, assembly and operation of the biochip are ideally suited for a disposable biochip specifically targeted towards POCT applications.
Owner:UNIVERSITY OF CINCINNATI
Smart disposable plastic lab-on-a-chip for point-of-care testing
InactiveUS7524464B2None of measures has been particularly successfulRelieve painBioreactor/fermenter combinationsCombination devicesVenous bloodLab-on-a-chip
Disclosed herein is a fully-integrated, disposable biochip for point-of-care testing of clinically relevant parameters. Specifically, in accordance with an embodiment of the present invention, the biochip is designed for POCT (point-of-care-testing) of an array of metabolic parameters including partial pressure of oxygen, Glucose, and Lactate concentration from venous blood samples. The biochip is fabricated on a low-cost plastic substrate using mass manufacturing compatible fabrication processes. Furthermore, the biochip contains a fully-integrated metallic micro-needle for blood sampling. The biochip also uses smart passive microfluidics in conjunction with low-power functional on-chip pressure generators for microfluidic sequencing. The design, configuration, assembly and operation of the biochip are ideally suited for a disposable biochip specifically targeted towards POCT applications.
Owner:UNIVERSITY OF CINCINNATI
Technique for separating and purifying L-lactic acid
InactiveCN101234962AIncrease profitIncrease concentrationFermentationCarboxylic compound separation/purificationDistillationUltrafiltration
The invention discloses a new technology for separating and purifying L-lactic acid with continuous ion exchange, membrane separation and molecular distillation. Centrifuged and decolorated lactic acid fermentation liquid is pumped into an ultrafiltration apparatus to remove proteins; clear liquid with a lactate concentration of 10-50g / L is then pumped into a continuous ion exchange system filled with anion exchange resin for adsorption; an inorganic salt solution with a concentration of 0.001-0.2M is used for impurity washing and an acid solution with a concentration of 0.1-2M is used for elution; nano-filtration technology is employed for synchronous concentration and desalination of the ion exchange eluent; high-purity and high-quality L-lactic acid can be obtained after treatment with molecular distillation means in the end. The technology has the advantages of low equipment investment, strong continuous operability, low production cost, high product purity, easy scale production, etc.
Owner:江苏道森生物化学有限公司
Blood testing method
ActiveUS20050074893A1Accurate diagnosisWithdrawing sample devicesDead animal preservationConcentrations glucoseBlood plasma
A blood test method including (a) centrifuging a mammalian blood sample into a plasma and blood cells; (b) removing buffy coats from the blood cells to obtain red blood cells containing solutes confined therewithin; (c) washing the red blood cells with a buffered physiological saline solution and isolating the washed blood cells; (d) mixing the washed red blood cells with a buffered physiological saline solution to obtain a suspended liquid; (e) centrifuging the suspended liquid to remove a supernatant and to obtain a red blood layer; (f) mixing the red blood layer with a hypertonic solution and maintaining the resulting suspension at a temperature of 25 to 40° C. for a period of time sufficient for the solutes confined in the red blood cells to penetrate into the hypertonic solution; (g) centrifuging the suspension to obtain a supernatant containing the solutes; and (h) measuring the supernatant for at least one factor selected from a glucose concentration, a pyruvic acid concentration, a lactic acid concentration and an oxidation-reduction potential.
Owner:RELTEC MEDICAL DEVICES CORPORATION
Frequently pole-reversing electroosmosis method for extracting and concentrating sodium lactate from garbage fermentation liquid
InactiveCN1820828AReduce pollutionExtended service lifeElectrodialysisCarboxylic compound separation/purificationElectricitySodium lactate
The present invention relates to sodium lactate extracting and concentrating method and apparatus, and aims at eliminating membrane contamination in sodium lactate extracting and concentrating electroosmosis process. The frequently pole-reversing electroosmosis process for extracting and concentrating sodium lactate from garbage fermenting liquid includes the following steps: 1. adding solution essential for extracting sodium lactate; 2. installing additional apparatus; 3. starting electroosmosis and reversing the polarity of the first electrode and the second electrode once every 10-30 min with the pole-reversing circuit; and 4. regulating the 4 two-position-three-way direction controlling valves to alter the work positions after each polarity reversing operation. The present invention has lowered membrane contamination, long service life of membrane, saving in cost, high lactate recovering rate up to 90 %, and concentrated lactate concentration up to 18 %.
Owner:HARBIN INST OF TECH
Method for detecting blood lactic acid in vitro by using chemiluminescence method
InactiveCN101639446AHigh sensitivityMinimum minimumMicrobiological testing/measurementChemiluminescene/bioluminescenceO-Phosphoric AcidGlycerol
The invention discloses a method for detecting blood lactic acid in vitro by using a chemiluminescence method, which is characterized in that LD is utilized to catalyze L-lactic acid and NAD<+> to generate pyruvate and NADH, the pyruvate and ADP generate ATP under the catalysis of pyruvate kinase (PK), ATP and glycerol generate glycerol-3-phosphoric acid and ADP under the catalysis of GK, the glycerol-3-phosphoric acid is acted by GPO to obtain H2O2, and H2O2 is catalyzed by HPR to enable luminol to emit light; the size of light signals is in positive correlation with the concentration of thepyruvate, i.e. the bigger the concentration of the lactic acid is, the stronger the emitted light signals are; the concentration of the lactic acid can be conjectured by recording the light signals; and the lactic acid with the known concentration is used for detecting the light signals to make a dose-response curve, and the content of the lactic acid of an unknown sample can be calculated throughthe curve. In the invention, a chemiluminescence substance replaces a colored substance to achieve the purposes of sensitivity, stability, wide range and safety. The method can be used for preparinga corresponding commercial kit for quantitatively detecting the lactic acid in body fluids such as whole blood, plasma, cerebrospinal fluid, urine, gastric juice and the like.
Owner:福建省洪诚生物药业有限公司
Lactate measuring device and method for training adjustment in sports
ActiveUS20150208970A1Painless and minimally-invasiveComplete efficientlyCatheterDiagnostic recording/measuringMeasurement deviceAthletic training
The invention relates to a method for training adjustment in sports. The method may measure lactate concentrations in the body of a person by a lactate measuring device comprising a transdermal microneedles sensor. The method for training adjustment in sports includes steps of: measuring a lactate concentration value in the body of a user by the transdermal microneedles sensor; comparing the lactate concentration value with a predetermined value; and informing the user to reduce exercise intensity if the lactate concentration value is higher than the predetermined value, otherwise informing the user to increase exercise intensity if the lactate concentration value is lower than the predetermined value. The invention also relates to a lactate measuring device.
Owner:RICHHEALTH TECH CORP +1
Slow-release antibacterial acellular matrix biological material and preparation method thereof
The invention provides a slow-release antibacterial acellular matrix biological material and a preparation method thereof. The preparation method comprises the following steps: S1, providing a biological tissue, and carrying out decellularization and virus inactivation treatment on the biological tissue; S2, performing plasma treatment on the treated biological tissue to modify the surface of the biological tissue to obtain an acellular matrix; S3, soaking the acellular matrix in a slow-release antibacterial aqueous solution containing an organic slow-release medium, an antibacterial agent and weak acid, so that the organic slow-release medium is loaded on the surface of the acellular matrix, the antibacterial agent is loaded on the surface of the organic slow-release medium, the weak acid is dilute acetic acid, dilute hydrochloric acid or dilute lactic acid, and the concentration of the weak acid is 0.01-1 mol / L; and S4, taking out the soaked acellular matrix, and performing vacuum freeze drying and sterilization to obtain the slow-release antibacterial acellular matrix biological material.
Owner:浙江德普斯医疗科技股份有限公司
Fetal lactic acid monitor
InactiveUS20120143087A1Prevent blood clottingAccurate and reliable indicationDiagnostic recording/measuringSensorsObstetricsScalp
A device and method for automatically and repeatedly measuring fetal Lactic Acid (LA) concentration in the blood, for monitoring fetal stress. The device is connected by a probe to the scalp of the fetus and measures the Lactic Acid concentration in the blood at preset intervals. The device is optionally provided with an alarm which is activated when lactic acid concentration is outside a preset normal range.
Owner:GANOR ZEEV +2
Lactate sensor
ActiveCN103018291AEfficient determinationMicrobiological testing/measurementBiological material analysisLactate oxidaseD lactate
The invention relates to a lactate sensor, and specifically a lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.
Owner:ARKRAY INC
Fermented soy-based beverage
InactiveCN101932250AReduce concentrationIncrease concentrationFood ingredientsVegetable proteins working-upLactic acid bacteriumPresent method
The present invention relates to a method of producing a beverage by: - providing a pasteurised or sterilised aqueous liquid containing 0.5-8 wt. % of dissolved soy protein and 0-0.2 wt.% of dairy protein; - inoculating the pasteurised or sterilised liquid with a thermophilic lactic acid bacterium containing starter culture; - fermenting the inoculated aqueous liquid by incubation at a temperature in the range of 40-48 DEG C for 0.5-24 hours to obtain a fermented product having a viscosity at a temperature of 7 DEG C of less than 50 mPa.s at 100 s-1; wherein in total less than 6% disaccharides by weight of the fermented product are added before, during or after fermentation and wherein during fermentation the following changes in concentrations of flavour compounds occur: lactate concentration increases with at least 500 ppm; diacetyl concentration increases with at least 0.3 ppm and / or acetaldehyde concentration increases with at least 0.05 ppm; concentration of n-hexanal decreases by at least 60%; at least two of n-pentanal concentration, n-heptanal concentration, n-octanal concentration and n-nonanal concentration decrease by at least 50%. The present method enables the effective removal of soy off-notes as well as the preparation of fermented substrates with a desirable flavour profile that is similar to that of fermented dairy products.
Owner:UNILEVER NV
Method and device for microdialysis sampling
ActiveUS20100152555A1Method stableReliable and safe and efficientVaccination/ovulation diagnosticsCatheterHeart operationsMetabolic state
Owner:MD BIOMEDICAL
Lactate sensor
ActiveUS20130075277A1Accurate measurementShort timeImmobilised enzymesBioreactor/fermenter combinationsLactate oxidaseReagent
A lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, it mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.
Owner:ARKRAY INC
Methods, media, and apparatus for optimizing physical training based on real-time blood lactate monitoring
Some embodiments described herein relate to receiving lactate concentration data from a non-invasive sensor. A ratio of aerobic to anaerobic exertion can be determined based on the concentration of lactate, and real-time feedback can be provided based on the ratio of aerobic to anaerobic exertion. For example, the user can be encouraged to maintain a level of intensity in which he or she approaches (but does not exceed) maximal oxygen consumption.
Owner:POTOMAC HEALTH SOLUTIONS
Method for monitoring a childbrith process
ActiveUS20060180161A1Prevent unnecessary agonizing and drawn out effortsSurgeryPerson identificationObstetricsAmniotic fluid
The method is for monitoring a childbirth process of a pregnant woman. In a measuring step, a lactate concentration of vaginal fluids is measured. In a comparison step, it is determined if the measured lactate concentration is greater than a predetermined lactate concentration that indicates that amniotic fluid has passed from an amnion of the pregnant woman. In a measuring step, the lactate concentration is measured. In a comparison step, it is determined if the measured lactate concentration is greater than a lactate threshold interval. When the lactate concentration is less than the lactate threshold interval the pregnant woman is stimulated in a stimulating step to give birth.
Owner:OBSTECARE AB
Full blood lactic biological sensor modified by oriented carbon nano tube
InactiveCN1648650AQuick storageImprove responsivenessMaterial electrochemical variablesReaction layerLactate oxidase
The full blood lactic acid biosensor modified with oriented carbon nanotube features that one layer of Co particles as the catalyst for oriented growth of carbon nanotube is deposited on the substrate and that the reaction layer has base layer comprising oriented carbon nanotube as the second electric intermediate and the upper layer comprising L-lactic oxidase and the mixture of reagent, stabilizer and buffering liquid as the first electric intermediate. Compared with available the technology, the present invention has the advantages of obviously raised responding sensitivity on lactic acid concentration of the sensor, work voltage of the sensor lowered from 500 mv to 300 mv, no need of pre-treatment of the blood sample and blood sample amount as less as 2 microliter.
Owner:ZHEJIANG UNIV
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324561AMicrobiological testing/measurementColor/spectral properties measurementsFerricytochrome cPeroxidase
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by utilizing a visible light analyzer and further reflecting the concentration of the D-lactic acid directly.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for determining the stress capacity of a person
The invention relates to a method for determining the stress capacity of a person taking into consideration the individual anaerobic threshold by measuring lactate concentrations in relation to physical effort. To make it possible to deduce from the changes in lactate concentration other performance data characteristic of the person tested, the invention provides for the following process steps: measurement of time-dependent lactate concentration changes above the individual anaerobic threshold; adjustment of the measurement cure, in which lactate concentration is recorded in relation to time, to the measurement values obtained in this way; determination of a first rise in the measurement curve at a time tIAT which corresponds to the individual anaerobic threshold: determination of at least one other rise in the measurement curve at a time tx where tx>tIAT and subtraction of the second rise from the first rise to determine a difference ΔA.
Owner:STEGMANN HEINER
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324560AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsFerricytochrome cAlanine dehydrogenase
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the D-lactic acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Chimeric receptor polypeptides in combination with trans metabolism molecules modulating intracellular lactate concentrations and therapeutic uses thereof
PendingCN112888786APeptide/protein ingredientsAntibody mimetics/scaffoldsHematopoietic cellAntiendomysial antibodies
Disclosed herein are genetically engineered hematopoietic cells, which express one or more lactate-modulating factors (e.g., polypeptides), and optionally a chimeric receptor polypeptide (e.g., an antibody-coupled T cell receptor (ACTR) polypeptide or a chimeric antigen receptor (CAR) polypeptide) capable of binding to a target antigen of interest. Also disclosed herein are uses of the engineered hematopoietic cells for inhibiting cells expressing a target antigen in a subject in need thereof.
Owner:ソティオリミティドライアビリティカンパニー
Method for sterilization of campylobacter on whole-chicken slaughtering processing production line
InactiveCN104255902AQuality is not affectedReduce in quantityMeat/fish preservation using acidsBiotechnologyAnimal science
The invention provides a method for sterilization of campylobacter on a whole-chicken slaughtering processing production line. The method can safely and effectively lower the quantity of the campylobacter on the surface of chicken, but not reduce the quality of the chicken. According to the method disclosed by the invention, lactic acid is adopted to process the whole chicken, wherein the optimal anti-bacterial conditions are as follows: the concentration of lactic acid is 0.19%, the soaking processing time is 17 minutes, and the soaking processing temperature is 20.9 DEG C. By adopting the method disclosed by the invention, the quantity of the campylobacter on the surface of the chicken processed by lactic acid reaches a lower level, and meanwhile the quality of the chicken is not influenced. Compared with the traditional disinfection method, the method is safer, more efficient and lower in influence on the chicken quality.
Owner:YANGZHOU UNIV
Process and system for propagating cell cultures while preventing lactate accumulation
PendingCN111630148ABioreactor/fermenter combinationsBiological substance pretreatmentsData miningBioreactor
A predictive model is described that can predict the parameter concentrations in the future based on initial, measured concentrations and historical data. A plurality of multivariate techniques can beused to construct the predictive model capable of forecasting concentrations over multiple and diverse cell lines. The predictive model is also scalable. In one embodiment, a future lactate concentration trajectory is determined, and at least one condition within a bioreactor is changed or modified to maintain lactate concentration within desired ranges.
Owner:LONZA LTD
Frequently pole-reversing electroosmosis method for extracting and concentrating sodium lactate from garbage fermentation liquid
InactiveCN100409926CReduce pollutionExtended service lifeElectrodialysisCarboxylic compound separation/purificationElectricitySodium lactate
The present invention relates to sodium lactate extracting and concentrating method and apparatus, and aims at eliminating membrane contamination in sodium lactate extracting and concentrating electroosmosis process. The frequently pole-reversing electroosmosis process for extracting and concentrating sodium lactate from garbage fermenting liquid includes the following steps: 1. adding solution essential for extracting sodium lactate; 2. installing additional apparatus; 3. starting electroosmosis and reversing the polarity of the first electrode and the second electrode once every 10-30 min with the pole-reversing circuit; and 4. regulating the 4 two-position-three-way direction controlling valves to alter the work positions after each polarity reversing operation. The present invention has lowered membrane contamination, long service life of membrane, saving in cost, high lactate recovering rate up to 90 %, and concentrated lactate concentration up to 18 %.
Owner:HARBIN INST OF TECH
Enterococcus faecalis strain and application thereof
The invention relates to an enterococcus faecalis strain for producing L-lactic acid and an application of the enterococcus faecalis strain. The enterococcus faecalis strain is classified and named asEnterococcus faecalis, is preserved in China General Microbiological Culture Collection Center on May 28,2018, and has a preservation number of CGMCC No.15821. The strain has high yield of L-lactic acid, can utilize a plurality of carbon sources, and has a little product inhibition effect. The lactic acid concentration is high, and particularly, optical purity of the L-lactic acid can reach 99 percent or above.
Owner:CHINA PETROLEUM & CHEM CORP +1
D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
InactiveCN101324555AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsEnzyme catalysisAbsorbance
The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technology of the enzymic colorimetric method based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, D-lactate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the D-lactic acid.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Lactate sensor
ActiveUS8999126B2Accurate measurementShort timeImmobilised enzymesBioreactor/fermenter combinationsLactate oxidaseReagent
A lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, it mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.
Owner:ARKRAY INC
Method for monitoring a childbirth process
ActiveUS7318809B2Prevent unnecessary agonizing and drawn out effortsSurgeryPerson identificationObstetricsAmniotic fluid
The method is for monitoring a childbirth process of a pregnant woman. In a measuring step, a lactate concentration of vaginal fluids is measured. In a comparison step, it is determined if the measured lactate concentration is greater than a predetermined lactate concentration that indicates that amniotic fluid has passed from an amnion of the pregnant woman. In a measuring step, the lactate concentration is measured. In a comparison step, it is determined if the measured lactate concentration is greater than a lactate threshold interval. When the lactate concentration is less than the lactate threshold interval the pregnant woman is stimulated in a stimulating step to give birth.
Owner:OBSTECARE AB
Lactate measuring device and method for training adjustment in sports
ActiveUS10034626B2Painless and minimally-invasiveComplete efficientlyCatheterSensorsMeasurement deviceAthletic training
The invention relates to a method for training adjustment in sports. The method may measure lactate concentrations in the body of a person by a lactate measuring device comprising a transdermal microneedles sensor. The method for training adjustment in sports includes steps of: measuring a lactate concentration value in the body of a user by the transdermal microneedles sensor; comparing the lactate concentration value with a predetermined value; and informing the user to reduce exercise intensity if the lactate concentration value is higher than the predetermined value, otherwise informing the user to increase exercise intensity if the lactate concentration value is lower than the predetermined value. The invention also relates to a lactate measuring device.
Owner:RICHHEALTH TECH CORP +1
Full blood lactic biological sensor modified by oriented carbon nano tube
InactiveCN100483122CQuick storageImprove responsivenessMaterial electrochemical variablesReaction layerLactate oxidase
The full blood lactic acid biosensor modified with oriented carbon nanotube features that one layer of Co particles as the catalyst for oriented growth of carbon nanotube is deposited on the substrate and that the reaction layer has base layer comprising oriented carbon nanotube as the second electric intermediate and the upper layer comprising L-lactic oxidase and the mixture of reagent, stabilizer and buffering liquid as the first electric intermediate. Compared with available the technology, the present invention has the advantages of obviously raised responding sensitivity on lactic acid concentration of the sensor, work voltage of the sensor lowered from 500 mv to 300 mv, no need of pre-treatment of the blood sample and blood sample amount as less as 2 microliter.
Owner:ZHEJIANG UNIV
Blood testing method
ActiveUS7276382B2Accurate diagnosisWithdrawing sample devicesDead animal preservationConcentrations glucoseWhite blood cell
A blood test method including (a) centrifuging a mammalian blood sample into a plasma and blood cells; (b) removing buffy coats from the blood cells to obtain red blood cells containing solutes confined therewithin; (c) washing the red blood cells with a buffered physiological saline solution and isolating the washed blood cells; (d) mixing the washed red blood cells with a buffered physiological saline solution to obtain a suspended liquid; (e) centrifuging the suspended liquid to remove a supernatant and to obtain a red blood layer; (f) mixing the red blood layer with a hypertonic solution and maintaining the resulting suspension at a temperature of 25 to 40° C. for a period of time sufficient for the solutes confined in the red blood cells to penetrate into the hypertonic solution; (g) centrifuging the suspension to obtain a supernatant containing the solutes; and (h) measuring the supernatant for at least one factor selected from a glucose concentration, a pyruvic acid concentration, a lactic acid concentration and an oxidation-reduction potential.
Owner:RELTEC MEDICAL DEVICES CORPORATION
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