Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Full blood lactic biological sensor modified by oriented carbon nano tube

A biosensor, carbon nanotube technology, applied in the field of biosensors, can solve the problems of interference of changes in oxygen content, poor anti-interference ability, poor redox characteristics, etc., to improve enzyme activity, improve response sensitivity, avoid Effects of changes in blood volume

Inactive Publication Date: 2005-08-03
ZHEJIANG UNIV
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defect of this sensor is: the voltage added to the electrode leads is relatively high, which is 0.5v, and the anti-interference ability is not good. better response current
Chinese patent CN1219676A introduces a disposable dual-electron mediator dual-enzyme biosensor for detecting cholesterol, glucose, etc. in the blood, but this sensor requires the participation of oxygen and is easily disturbed by changes in oxygen content in the blood

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Full blood lactic biological sensor modified by oriented carbon nano tube
  • Full blood lactic biological sensor modified by oriented carbon nano tube
  • Full blood lactic biological sensor modified by oriented carbon nano tube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] A whole blood lactic acid biosensor modified by oriented carbon nanotubes, the bottom plate 1 is anodized to form Al 2 o 2 The aluminum plate of the insulating layer, and the electrochemical method is used in the Al 2 o 3 Co particles that act as catalysts for the growth of oriented carbon nanotubes are deposited in the longitudinal micropores of the insulating layer; the electrode 2 is an Au electrode formed on the bottom plate 1 by vacuum sputtering, and the cavity 3 is covered with a plastic film around the electrode 2 Surrounded by a capillary hole 3a for sampling on one of its side walls, the reaction layer 4 is arranged in the cavity 3, and its bottom layer is an aligned carbon nanotube layer 4a used as a second electron mediator. The oriented carbon nanotube layer is obtained by chemical vapor deposition. The oriented growth time is 5 minutes, and the ultrasonic cut-off time is 4 hours. L-lactate oxidase and reagents, stabilizers, and buffers as the first elect...

Embodiment 2

[0013] A whole blood lactic acid biosensor modified by oriented carbon nanotubes, the bottom plate 1 is anodized to form Al 2 o 2 The aluminum plate of the insulating layer, and the electrochemical method is used in the Al 2 o 3 Co particles that act as catalysts for the growth of oriented carbon nanotubes are deposited in the longitudinal micropores of the insulating layer; the electrode 2 is an Au electrode formed on the bottom plate 1 by vacuum sputtering, and the cavity 3 is covered with a plastic film around the electrode 2 Surrounded by a capillary hole 3a for sampling on one of its side walls, the reaction layer 4 is arranged in the cavity 3, and its bottom layer is an aligned carbon nanotube layer 4a used as a second electron mediator. The oriented carbon nanotube layer is obtained by chemical vapor deposition. The oriented growth time is 5 minutes, and the ultrasonic cut-off time is 8 hours. The upper layer is dripped with L-lactate oxidase and reagents, stabilizers...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The full blood lactic acid biosensor modified with oriented carbon nanotube features that one layer of Co particles as the catalyst for oriented growth of carbon nanotube is deposited on the substrate and that the reaction layer has base layer comprising oriented carbon nanotube as the second electric intermediate and the upper layer comprising L-lactic oxidase and the mixture of reagent, stabilizer and buffering liquid as the first electric intermediate. Compared with available the technology, the present invention has the advantages of obviously raised responding sensitivity on lactic acid concentration of the sensor, work voltage of the sensor lowered from 500 mv to 300 mv, no need of pre-treatment of the blood sample and blood sample amount as less as 2 microliter.

Description

technical field [0001] The invention relates to a biosensor, in particular to a whole blood lactic acid biosensor modified by directional carbon nanotubes. Background technique [0002] Whole blood lactic acid biosensor modified by oriented carbon nanotubes is a biosensor that uses biomolecules as sensitive elements to quickly, accurately and conveniently measure lactic acid content in blood and other liquids. This sensor is used to measure lactic acid content in human blood. It is used to reveal the severity of certain diseases, such as guiding diabetic patients to take Jiangtangling appropriately. Excessive use of Jiangtangling will cause glycolysis, increase the lactic acid content, cause acidosis, and endanger the patient's life; it is used to measure the concentration of lactic acid in athletes, It can scientifically control the training intensity of athletes to achieve the best training effect; in food science, the freshness of food can be determined by detecting the c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/26G01N27/327G01N27/403
Inventor 陈裕泉潘敏陈文菊陈金霞
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com