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D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration

A technology of concentration determination and kit, which is applied in the field of medical/food inspection and determination, and can solve problems such as microbial contamination

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If D-lactic acid is present, microbial contamination may be the cause

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The D-lactic acid diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0029] Glycine-sodium hydroxide buffer 100mmol / L

[0030] Stabilizer 500mmol / L

[0031] Coenzyme 3mmol / L

[0032] D-lactate dehydrogenase 12000U / L

[0033] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0034] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured D-lactic acid sample to the reagent is 1 / 25 , the reaction direction is forward reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0035] After adding the samples and reagents, let them mix and react, and finally put the reactants under the biochemical analyzer to d...

Embodiment 2

[0037] The D-lactic acid diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0038] Reagent 1

[0039] Glycine-sodium hydroxide buffer 100mmol / L

[0040] Stabilizer 50mmol / L

[0041] Coenzyme 3mmol / L

[0042] Reagent 2

[0043] Glycine-sodium hydroxide buffer 100mmol / L

[0044] Stabilizer 500mmol / L

[0045] D-lactate dehydrogenase 12000U / L

[0046] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0047] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured D-lactic acid sample to reagent 1 and reagent 2 It is 2 / 20 / 5, the reaction direction is positive reaction (rising reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0048] After adding the samples and rea...

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Abstract

The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technology of the enzymic colorimetric method based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, D-lactate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the D-lactic acid.

Description

technical field [0001] The invention relates to a D-lactic acid diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of D-lactic acid, which belongs to the technical field of medical / food inspection and determination. Background technique [0002] L-lactic acid is an organic acid that has attracted much attention and developed rapidly in recent years. At the same time, L-lactic acid has application prospects in many fields such as food, feed, medicine, plastics, feed, pesticide, daily chemical industry, papermaking and electronic industry. [0003] L-lactic acid is the end product of the metabolism of most organisms. Only a few microorganisms (such as Lactobacillus, Leuconostoc) can form D-lactic acid. Great care is taken in the production of yogurt to detect lactate to assess whether the microbes are producing L-lactic acid, D-lactic acid or both. If D-lactic acid is present, microbial contamination may b...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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