D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
A kit, lactic acid technology, applied in the field of medical/food testing and determination, can solve problems such as microbial contamination
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Embodiment 1
[0100] The D-lactic acid diagnosis / measurement reagent of this embodiment is a single reagent, including:
[0101] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0102] Stabilizer 500mmol / L
[0103] Reduced coenzyme 0.25mmol / L
[0104] 2 Ferricytochrome c 10mmol / L
[0106] D-lactate dehydrogenase 10000U / L
[0107] Alanine dehydrogenase 10000U / L
[0108] Glycine oxidase 10000U / L
[0109] Peroxidase 30000U / L
[0110] 4-amino antiarsenic 2mmol / L
[0111] Carbolic acid 10mmol / L
[0112] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.
[0113] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the measured D-lactic acid sample to the reagent is 1 / 25, and the reaction direction is...
Embodiment 2
[0116] The D-lactic acid diagnosis / measurement reagent of this embodiment is a double reagent, including:
[0117] Reagent 1
[0118] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0119] Stabilizer 50mmol / L
[0120] Reduced coenzyme 0.25mmol / L
[0121] 2 Ferricytochrome c 10mmol / L
[0123] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L
[0124] Reagent 2
[0125] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0126] Stabilizer 500mmol / L
[0127] D-lactate dehydrogenase 10000U / L
[0128] Alanine dehydrogenase 10000U / L
[0129] Glycine oxidase 10000U / L
[0130] Peroxidase 25000U / L
[0131] 4-amino antiarsenic 0.6mmol / L
[0132] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.
[0133] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 54...
Embodiment 3
[0136] The D-lactic acid diagnosis / measurement reagent of the present embodiment is three reagents, comprising:
[0137] Reagent 1
[0138] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0139] Stabilizer 50mmol / L
[0140] Reduced coenzyme 0.25mmol / L
[0141] 2 Ferricytochrome c 10mmol / L
[0143] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L
[0144] Reagent 2
[0145] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0146] Stabilizer 500mmol / L
[0147] Alanine dehydrogenase 10000U / L
[0148] Glycine oxidase 10000U / L
[0149] Peroxidase 20000U / L
[0150] Dimethylaniline 2mmol / L
[0151] Reagent 3
[0152] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0153]Stabilizer 500mmol / L
[0154] D-lactate dehydrogenase 10000U / L
[0155] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.
...
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