D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration
A kit, lactic acid technology, applied in the field of medical/food testing and determination, can solve problems such as microbial contamination
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0042] The D-lactic acid diagnostic / measurement reagent in this embodiment is a single reagent, including:
[0043] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0044] Stabilizer 500mmol / L
[0045] Reduced coenzyme 0.25mmol / L
[0046] 2 high iron cytochrome c 10mmol / L
[0047] Carbon dioxide 20mmol / L
[0048] Lactate dehydrogenase 10000U / L
[0049] Malate dehydrogenase 16000U / L
[0050] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.
[0051] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested D-lactic acid sample to reagent is 1 / 25. The reaction direction is negative reaction (declining reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes....
Embodiment 2
[0054] The D-lactic acid diagnostic / measurement reagent in this embodiment is a dual reagent, including:
[0055] Reagent 1
[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0057] Stabilizer 50mmol / L
[0058] Reduced coenzyme 0.25mmol / L
[0059] 2 high iron cytochrome c 10mmol / L
[0060] Carbon dioxide 20mmol / L
[0061] Reagent 2
[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0063] Stabilizer 500mmol / L
[0064]Lactate dehydrogenase 10000U / L
[0065] Malate dehydrogenase 16000U / L
[0066] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.
[0067] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, volume of D-lactic acid sample and reagent 1, reagent 2 The ratio is 2 / 20 / 5, the reaction direction is negative reaction ...
Embodiment 3
[0070] The D-lactic acid diagnostic / determination reagent in this embodiment is three reagents, including:
[0071] Reagent 1
[0072] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0073] Stabilizer 50mmol / L
[0074] Reduced coenzyme 0.25mmol / L
[0075] 2 high iron cytochrome c 10mmol / L
[0076] Carbon dioxide 20mmol / L
[0077] Reagent 2
[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0079] Stabilizer 500mmol / L
[0080] Malate dehydrogenase 16000U / L
[0081] Reagent 3
[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0083] Stabilizer 500mmol / L
[0084] Lactate dehydrogenase 10000U / L
[0085] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.
[0086] When determining the concentration of D-lactic acid, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1....
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com