The invention discloses an iris positioning method and device. Among them, the method includes: obtaining the image information of the target iris, and roughly positioning the pupil in the image information; constructing a tripartite map of the target object according to the rough positioning result of the pupil, and using a preset algorithm to obtain extract the target object, wherein the target object includes: the pupil, eyelid and iris outer circle of the target iris; determine the positioning information of the target iris according to the extracted pupil, eyelid and iris outer circle, wherein the positioning information includes at least one of the following: pupil border, eyelid border, and iris outer border. The invention solves the technical problem of inaccurate iris positioning results in the prior art when the iris image is a non-ideal iris image such as non-circular pupils, uneven illumination, over- or under-exposure, and the like.