37 results about "Infectious peritonitis" patented technology

The invention provides a method for quickly detecting Chinese papaya leaf curl virus by LAMP (loop mediated isothermal amplification), belonging to the field of plant protection. The method comprises the following steps of: designing an LAMP primer (P-F3, P-B3, P-FIP (feline infectious peritonitis) and P-BIP (bit interleaved parity)) according to the nucleotide sequence of the Chinese papaya leaf curl virus; performing the LAMP reaction after total DNA (deoxyribonucleic acid) is extracted from a sample; and performing the electrophoresis or the fluorescent dye to a reaction product. The method is simple to operate, some special apparatuses such as a PCR apparatus and the like are avoided, and the problems of the conventional methods such as the biology, the serology and the like are overcome, so that the detection method is simple, fast, sensitive and economical.

The present invention relates to a reliable method of prediction of infectious peritonitis in humans, wherein the level of pancreatic stone protein/regenerating protein (PSP/reg) is determined in serum or plasma, and a high level is indicative of the development and the severity of peritonitis, allowing the classification of patients according to risk.

The invention discloses application of FHPI for preparing a medicine for treating feline infectious peritonitis. FHPI has the little toxic effect on normal feline cells, has the obvious inhibition effect on feline coronaviruses, and can obviously lower the virulence of viruses in cells; indirect immunofluorescence light and Western Blot determination results show that FHPI can inhibit proliferation of feline coronaviruses in cells, and has the dose dependence. Accordingly, the FHPI has the activity for resisting feline coronaviruses, can be used for preparing a feline coronavirus inhibitor, can be used for treating feline infectious peritonitis and has the advantages of being good in curative effect, high in safety and the like.

The invention provides an HRM primer pair, kit and method for rapidly identifying feline enteric coronavirus and feline infectious peritonitis viruses, and belongs to the technical field of molecularbiological detection. The high-resolution melting (HRM) primer pair disclosed by the invention is designed according to a sequence between 29000bp and 29500bp of a full-length gene of feline coronavirus (FCOV). The feline enteric coronavirus (FECV) and the feline infectious peritonitis viruses (FIPV) can be identified and distinguished at the same time through melting curves with different shapesby one-time reaction, and technical support is provided for early diagnosis and prevention and control of diseases.

The invention provides a traditional Chinese medicine composition for treating feline infectious peritonitis, which comprises the following traditional Chinese medicine components: radix puerariae, Snphora angustifolia, radix scutellariae, Herba Lycopi, dandelion, radix bupleuri, milk veteh, and radix codonopsitis. According to the method of Chinese veterinary medicine dialectical therapy, radix puerariae has the effects of releasing the exterior and relieving the muscles and skin, removing fever and promoting eruption, promoting the production of body fluid to quench thirst, lifting yang and relieving diarrhea; Snphora angustifolia has the effects of clearing heat and drying damp, dispersing pathogenic wind, killing parasites to relieve itching and promoting urination; radix scutellariae has the effects of clearing heat and drying damp, purging intense heat and detonicating; Herba Lycopi has the effects of promoting blood circulation by removing blood stasis and inducing diuresis for removing edema; dandelion has the effects of clearing away heat and toxic material and removing swelling and stasis; radix bupleuri has the effects of expelling surficial evils and heat-evil, soothing liver-qi stagnation and lifting yang; milk veteh has the effects of invigorating qi for securing the exterior, draining water and reducing swelling, draining pus and expelling toxin and promoting tissue regeneration; and radix codonopsitis has the effects of tonifying middle-Jiao and Qi, strengthening the spleen and replenishing lung. According to combined action of radix puerariae, Snphora angustifolia, radix scutellariae, Herba Lycopi, dandelion, radix bupleuri, milk veteh, and radix codonopsiti, feline infectious peritonitis can be efficiently treated.

The invention discloses a novel method for diagnosing feline infectious peritonitis. A kit adopted in the method comprises a primer shown as SEQ ID NO.1-2 and two amino acid differences shown as SEQ ID NO.3-4. The invention further provides a new thought for identifying feline infectious peritonitis (FIPV) and feline enteric corona virus (FECV). By means of the detection scheme, accurate, specificand sensitive result judgment which cannot be completed through an existing detection technology can be achieved, the detection accuracy on feline infectious peritonitis is remarkably improved, and important technical support is provided for discovering and effectively preventing and controlling feline infectious peritonitis as soon as possible.

The present invention provides compositions comprising granulocyte-macrophage colony-stimulating factor and antimicrobial agents for the treatment, pre-emptive treatment or prevention of infectious peritonitis or intra-abdominal infection by intraperitoneal administration of the compositions.

The invention belongs to the technical field of medicines, and particularly relates to application of a bromophenol-pyrazoline compound in treatment of feline coronavirus diseases. The research finds that the bromophenol-pyrazoline compound can inhibit the activity of feline infectious peritonitis virus main protease (FIPV Mpro) and interfere replication of Feline Infectious Peritonitis Virus (FIPV) in cells. In clinical experiments, the bromophenol-pyrazoline compound can treat feline infectious peritonitis naturally infected by the FIPV, greatly improves the survival rate of sick cats, and can be used for preparing medicines for treating the feline infectious peritonitis.

The invention belongs to the technical field of gene recombination and antibody preparation, and particularly discloses a cat infectious peritonitis egg yolk antibody composition as well as a preparation method and application thereof. The composition is obtained by mixing a feline intestinal coronavirus S protein egg yolk antibody, a type I feline infectious peritonitis virus S protein egg yolk antibody and a type II feline infectious peritonitis virus S protein egg yolk antibody according to the mass ratio of (1-2): (1-2): (1-2) under the same titer, and compared with a single antibody, the two antibodies are mixed to form the composition. And the three antibodies are mixed according to other proportions, so that the effect of treating feline infectious peritonitis is good. The traditional Chinese medicine composition not only can neutralize cat intestinal coronavirus infection without clinical symptoms so as to prevent cat infectious peritonitis virus infection, but also can neutralize infection of cat infectious peritonitis type I and type II with clinical symptoms so as to achieve the effect of treating cat infectious peritonitis. And particularly, the compound has a relatively good treatment effect on cross infection of type I and type II cat infectious peritonitis viruses.

The present invention provides compositions comprising granulocyte-macrophage colony-stimulating factor and antibiotic agents to treat, pre-emptively treat or prevent infectious peritonitis or intra-abdominal infection and reduce the occurrence of local peritonitis or intra-abdominal abscess resulting therefrom by the intraperitoneal administration of the compositions.