46 results about "Haemoglobin A1c" patented technology

Provided is a device and a method, said device enabling the measurement of both of blood glucose level and hemoglobin A1c level, being small-sized and thus portable, allowing the measurement of a small amount of a specimen and, therefore, being usable in POC. The device for measuring the blood glucose level and hemoglobin A1c level of a blood specimen comprises: a test piece-mounting section (1) for detachably mounting test pieces (200) on which the blood specimen is to be spotted; a light-emitting section (2) for emitting light for irradiating the test pieces (200); a light-receiving section (3) for receiving reflected lights from the test pieces (200); and an operating section (4) for calculating the blood glucose level and hemoglobin A1c level of the blood specimen on the basis of photometric values obtained from the light-receiving section (3). As a test piece (200) for measuring the blood glucose level of the blood specimen, a test piece (A), which carries a composition (a) reacting with glucose and showing color development, is mounted. As a test piece (200) for measuring the hemoglobin A1c level of the blood specimen, a test piece (B), which carries a composition (b) reacting with glycated hemoglobin and showing color development, is mounted.

The purpose of the present invention is to provide: a method for measuring hemoglobins, which can measure hemoglobins within a short time and with high accuracy; and a method for measuring hemoglobin A1c, a method for measuring hemoglobin A1c and hemoglobin F simultaneously, a method for measuring hemoglobin A1c and hemoglobin A2 simultaneously and a method for measuring hemoglobin A1c and an abnormal hemoglobin simultaneously, each of which utilizes the above-mentioned method for measuring hemoglobins. The present invention is a method for measuring hemoglobins by liquid chromatography using a column which has cation-exchangeable particles filled therein as a column filler and has a pressure value of 9.8 plus103 to 29.4 plus105 Pa inclusive when an eluent to be used for the measurement is fed therethrough at a flow rate of 1.0 mL / minute, wherein the cation-exchangeable particles comprise crosslinked polymer particles and a polymer containing a cation-exchange group and provided on the surfaces of the crosslinked polymer particles.

Means that enables an immunoassay of hemoglobin A1c in a whole blood sample without pretreatment of the sample is disclosed. The immunoassay of hemoglobin A1c in a sample according to the present invention comprises bringing latex particles into contact with a non-pretreated whole blood sample in a hypotonic solution, and then bringing hemoglobin A1c adsorbed on the latex particles into contact with an anti-hemoglobin A1c antibody. The immunoassay is preferably carried out by an agglutination method. The hypotonic solution is, for example, a buffer containing a Good's buffer at a concentration of 0.02 to 0.2 mol / L, which Good's buffer has the maximum buffer capacity in the neutral to alkaline region.

Provided is an amadoriase characterized in acting on the beta-chains of hemoglobin A1c (HbA1c) to generate hydrogen peroxide. A HbA1c measurement method and measurement reagent kit using same are also provided. A HbA1c measurement method uses an amadoriase characterized in having a specific activity of 0.1 U / mg or more with respect to alphaF6P and in oxidizing the amino terminus of the beta-chains of HbA1c to generate hydrogen peroxide, and a measurement reagent kit comprises said amadoriase. The present invention provides a measurement method and measurement kit that are able to quantify HbA1c rapidly, easily and with good precision.

Provided is a novel means for suitably maintaining the dispersion state of a latex reagent in an immunoassay kit and preventing the occurrence of coagulation and sedimentation due to changes in temperature so that a reagent is protected from deterioration in measurement performance, said means also being applicable to immunoagglutination measurement kits in which a small amount of a latex reagent is dispensed. An immunoassay reagent according to the present invention comprises unsensitized latex particles and trimethyl glycine in a solvent. The concentration of trimethyl glycine in the reagent is preferably 5-10 w / v%. The reagent can be suitably used as an immunoassay reagent for hemoglobin A1c, for example.

An object of the present invention is to provide a method for measuring hemoglobin that enables short-time, high-accuracy measurement of hemoglobin, in particular stable hemoglobin A1c, which is used as a diagnostic indicator of diabetes mellitus, and an electrophoresis apparatus that is suitably used in this measurement method. The present invention provides a method for measuring hemoglobins using electrophoresis, which includes: using a migration path having an inner surface coated with a cationic substance to be immobilized on the inner surface or a migration path having an inner surface made of a cationic material; and using a buffer solution containing a water-soluble polymer having an anionic group as an electrophoresis buffer solution.

The disclosure also relates to novel compositions and methods for the single, direct detection of Hemoglobin A1c percentage in a sample, using conversion of functional groups attached to a transitional metal complex, resulting in quantifiable electrochemical signal at two unique potentials, Eo1 and Eo2.