Method for measuring hemoglobins
A hemoglobin and measurement method technology, applied in separation methods, chemical instruments and methods, biological testing, etc., can solve the problems of decreased measurement accuracy, chromatogram deformation, and greater influence of pressure values, etc.
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Embodiment 1
[0118] 200 g of tetraethylene glycol dimethacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), 50 g of diethylene glycol dimethacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.), and tetramethylolmethane triacrylate (manufactured by Shin-Nakamura Chemical Industry Co., Ltd.) 1.0 g of benzoyl peroxide (manufactured by NACALAI TESQUE, INC.) was dissolved in 100 g of Nakamura Chemical Industry Co., Ltd. The obtained mixture was dispersed in 2 L of an aqueous solution of 5% by weight of polyvinyl alcohol (manufactured by Nippon Synthetic Chemical Industry Co., Ltd., "Gohsenol GH-20"), and heated under a nitrogen atmosphere while stirring at a rotation speed of 300 rpm using a rotating paddle. The polymerization reaction was carried out at 80° C. for 1 hour. After 1 hour, 200 mL of an aqueous solution in which 80 g of acrylamide-tert-butylsulfonic acid (manufactured by Toagosei Co., Ltd.) was dissolved was added to the reaction system, and polyme...
Embodiment 2~4
[0143]Polymerization was performed by reducing the rotation speed during stirring during the polymerization reaction in Example 1, and a column packing material having a volume average particle diameter different from that in Example 1 was prepared. The volume average particle diameter and the column pressure value were measured by the same method as in Example 1. Table 1 shows the set rotational speed during polymerization, the volume average particle diameter, the size of the column used, the column pressure value, the flow rate during the measurement, and the pressure during the measurement. In addition, these conditions and measurement results in the following examples and comparative examples are also shown in Table 1.
[0144] Using the obtained column, the normal human blood used in Example 1, the sample containing abnormal hemoglobin, and the sample containing hemoglobin A2 were measured. figure 2 , image 3 , Figure 4 Same chromatogram.
[0145] Table 1 shows th...
Embodiment 5、6
[0147] In Examples 5 and 6, a column containing a polymer having a cation exchange group on the surface of the crosslinked polymer particle and satisfying the "regular pressure value of the column" of the present invention was prepared. However, an example is shown in which hemoglobins are measured under conditions that do not comply with the "prescribed pressure value at the time of measurement" of the present invention.
[0148] Polymerization was carried out by increasing the rotation speed during stirring during the polymerization reaction in Example 1 to prepare cation-exchange particles having different volume average particle diameters. The volume average particle diameter and the column pressure value were measured by the same method as in Example 1. Table 1 shows the set rotation speed, volume average particle diameter and column pressure during polymerization.
[0149] Using the obtained column, the normal human blood used in Example 1, the sample containing abnorma...
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