The invention relates to the field of petroleum geology and geotechnical engineering, in particular to a fracture surface roughness anisotropy characterization method. Methods and devices of a rock surface topography instrument, a contact punch instrument, a digital surface roughness measuring instrument, a photograph measurement method, a three-dimensional laser scanner and the like are used forobtaining three-dimensional information data of a fracture surface; by building fracture surface roughness evaluation units of different directions and a standard joint profile database, a fracture surface cross-scale evaluation model is built, and characterization of the fracture surface roughness anisotropy is achieved. According to the fracture surface roughness anisotropy characterization method, by building a corresponding mathematical algorithm, programmed characterization of the fracture surface roughness anisotropy is achieved, the fracture surface roughness anisotropy characterizationmethod has a high application value, moreover, the prediction cost is low, the operability is high, large labor and financial resource expenditure can be reduced, and a prediction result has important significance in multiple aspects of evaluating application of the mechanical property of the fracture surface in engineering practice, petroleum geology and the like.