The invention discloses a multi-degree-of-freedom micromanipulator which is driven by a multi-polarization mode piezoelectric actuator. The micromanipulator comprises a substrate, a nested x and y axis translation micromotion mechanism, a Z axis translation micromotion mechanism, a Z axis turning micromotion mechanism and an end executive mechanism, wherein three-dimensional translation is realized by using a piezoelectric pile actuator with a piezoelectric strain constant d33 driving a flexure hinge; Z axis turning is realized by using a piezoelectric turning actuator with a piezoelectric strain constant d15 driving a reverse arm; and the end executive mechanism is a micro clamp holder consisting of a piezoelectric double-chip cantilever beam based on a piezoelectric strain constant d31 or a micro injection probe. High accurate micromotions such as the three-dimensional translation, turning around with a Z axis and swing around with an X axis are realized by the drive of the piezoelectric actuator in different polarization modes, and operations such as clamping, transport, probing, injection and the like are realized by different end executive arms. The micromanipulator has the advantages of freedom decoupling, compact structure, flexible operation, convenient control, high motion precision and the like, and the micromanipulator can be applied in the fields of biomedicine, micro electro mechanical engineering and the like.