40 results about "Endoglucanase Y" patented technology

It is an object of the present invention to provide enzymes that have high endoglucanase activity and yet exhibit high activity even under alkaline conditions, and genes encoding the same. The enzyme according to the invention has the following properties: a) exhibiting endoglucanase activity; and b) capable of completely removing fuzz from regenerated cellulose fabrics at a concentration of 1 mg of the protein / L or below. The enzyme of the invention having endoglucanase activity is a protein comprising the amino acid sequence as shown in SEQ ID NO: 1, 3, 5, 7, 9 or 11; a modified protein thereof exhibiting endoglucanase activity; or a homologue of the protein or the modified protein.

A novel endoglucanase derived from Staphylotrichum coccosporum, a polynucleotide encoding the endoglucanase, and a cellulase preparation containing the endoglucanase are disclosed. The endoglucanase or cellulase preparation is available for a washing use or fabric processing, such as a color clarification of a cellulose-containing fabric, a reduction of fuzz, an improvement of the touch feel and appearance of the fabric, providing a localized color change to the fabric, or a reduction of stiffness.

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.

Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing.

The invention relates to a method for preparing microcrystalline cellulose and fiber ethanol by separating furfural residues, which comprises the following steps: (1) ethanol is taken as a solvent, a circulating ultrasonic extraction method is adopted to extract, and lignin is separated from an extraction liquid; (2) extraction residues adopt complex enzyme of endoglucanase and beta-glucosaccharase for enzyme-catalyzed hydrolysis, and a hydrolysate is fermented to prepare the ethanol; and (3) hydrolysis residues are subjected to acid-catalyzed hydrolysis to separate the microcrystalline cellulose. The extraction method has the advantages that the extraction method is green and environment-friendly, the enzyme-catalyzed hydrolysis has mild conditions, the side effect is small, the acid concentration needed by acid hydrolysis is low, the action time is short, and the yield of each step is high; and the method has smart process combination, can greatly reduce the production cost for the microcrystalline cellulose and the fiber ethanol by utilizing a special structure of furfural waste residues, and has double benefits of economization and environmental protection.

The present invention relates to a detergent composition comprising an endo-glucanase that provides I improved detergency performance. The invention also relates to a detergent composition comprising a combination of an endo-glucanase and other enzymes. One aspect of the invention relates to a process of washing a fabric of hard surface with the endo-glucanase detergent.

An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and β-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and β-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.

The invention discloses basophilic streptomyce, neutral endoglucanase produced by basophilic streptomyce and application of basophilic streptomyce. Basophilic streptomyce is a strain which is screened from soil of mangrove in Shenzhen and is capable of producing the neutral endoglucanase. The neutral endoglucanase has excellent enzymatic characteristics, is applicable to industries of washing and papermaking, is wide in pH application range and capable of resisting heat, alkali, metal ions and surfactants and can be applied to industries of washing and papermaking. Basophilic streptomyce belongs to an alkaline-resisting extreme microorganism, can be cultured at high-alkaline conditions and is capable of effectively restraining microbial contamination and beneficial to large-scale fermentation production.

The invention provides a bacillus subtilis strain SB13 which is deposited in the China General Microbiological Culture Collection Center in February 28, 2014 and which has an accession number of CGMCC No.8867, and discloses a composite liquid prepared by the bacillus subtilis strain SB13 and containing endoglucanase and xylanase simultaneously, namely the bacillus subtilis strain SB13 can ferment and grow by adopting bean curd waste water as the only nutrition compound, and produce an enzyme solution containing the endoglucanase and the xylanase, thus providing a route of recovery and reutilization of the bean curd waste water, and providing a new fermentation bacterial source for production of endoglucanase and xylanase preparations in the feed processing industry.

The invention relates to a bacillus licheniformis strain and application thereof. The strain is a bacillus licheniformis strain BL14 (Bacillus licheniformis) which is preserved in CCMCC on 28th, February, 2014, wherein the preservation number is CCMCC NO. 8865. The application of the bacillus licheniformis strain comprises the following steps: collecting bean curd wastewater; adjusting the pH to 7.0; putting at 121 DEG C and autoclaving for 25 minutes; inoculating the bacillus licheniformis strain BL14 to the bean curd wastewater according to an inoculation quantity of 106cuf / mL; aerating; culturing for 3 days at 33 DEG C and fermenting the bean curd wastewater to a compound enzymatic liquid. The bacillus licheniformis can ferment and grow by taking the bean curd wastewater as a unique nutritional substance and produce an enzymatic liquid of endoglucanase and xylanase, so that a novel fermenting bacterial source is provided for producing endoglucanase and xylanase preparations.

The invention discloses a Bacillus megaterium strain and application thereof. The strain is Bacillus megaterium strain BM5, is preserved in the Chinese Culture Collection Committee General Microbiology Center on February 28, 2014, and has a preservation number of CCMCC NO.8866; the application of the Bacillus megaterium strain comprises the following steps: collecting tofu wastewater, adjusting pH to 7.0, placing the tofu wastewater in a fermentation tank to perform autoclaved sterilization for 25 min at 121 DEG C, inoculating 106 cfu / mL of Bacillus megaterium strain BM5 in the fermentation tank to perform aeration, culturing the strain for 3 days at 33 DEG C to obtain a composite enzyme solution through fermentation. According to the invention, the Bacillus megaterium strain can ferment and grow by using the tofu wastewater as the unique nutrient, and can produce the enzyme solutions of endoglucanase and xylanase, so as to provide a new fermentation bacteria source for the production of endoglucanase and xylanase preparations.

The invention discloses an alkaliphilic bacillus, in particular to an endoglucanase strain screened out from the alkaline sullage in the paper mill. The invention also discloses a neutral endoglucanase and an alkaline endoglucanase. The two endoglucanases can be applied in the detergent and textile industries. The alkaliphilic bacillus belongs to alkaliphilic extremophiles. Compared with the prior industrial strains, the invention can be cultured under the condition of high alkalinity, which has the advantages that mixed fungi pollution can be effectively inhibited and large-scale fermentation production is favored.

The invention relates to Penicillium citrinum CR-2CGMCC 3024 and an application thereof. The bacterium is Penicillium fungus, which has short cultivating period and high propagation speed. An abundant of uniform cellulose degradation enzymes can be cultivated at short time for generating fungus culture solution. The invention also provides a method for generating endoglucanase and exoglucanase by fermenting the bacterial strain in the liquid enzyme culture medium, which can produce endoglucanase and exoglucanase with higher vigor at short time, with simple and stable fermenting technique, low cost and high yield.

The invention discloses a trichoderma reesei engineering bacterium high in secretion of endoglucanase and beta-glucosidases. The trichoderma reesei engineering bacterium is named as trichoderma reesei(Trichoderma reesei) QMEB5, the genotype of the bacterial strain is QM9414 delta Eegl2 delta Ebgl1, trichoderma reesei QM9414 is used as an original strain, the expression vector pTeg2 of a fusion gene of a gene expression box containing endoglucanase 2 is transformed, then the plasmid pTHB of a gene expression box containing beta-glucosidases is transformed, and screening and verifying are performed, so that the trichoderma reesei engineering bacterium is obtained. The invention further discloses an application of the engineering strain to fermentation production of cellulases. The experiment confirms that the egl2 expression quantity of the engineering strain is 27 times higher than that of the original strain, the bgl1 expression quantity of the engineering strain is 410 times higher than that of the original strain, the activity of the endoglucanase is 117.5IU / mL, and the activity of the beta-glucosidases is 34.5IU / mL. The cellulase system produced through fermentation can be efficiently applied to degradation of corn cobs and other lignocellulolyticenzyme materials, and has favorable industrial development and application prospects.

There is provided an endoglucanase enzyme, which is useful for reducing fuzz of regenerated cellulose-containing fabrics, improving the touch and appearance, color clarification, localized variation in color, reducing stiffness and using it as components of a detergent, as well as deinking waste paper and improving freeness of paper pulp. A cDNA coding for the endoglucanase enzyme NCE5 was cloned and its DNA sequence and amino acid sequence derived from it were determined.

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.

This invention provides a method for enhancing the cellobiase activity of the strain Termitimyces clypeatus using 2-deoxy-D-glucose as glycosylation inhibitor, said process comprises inoculating and growing mycelial culture of (the edible mushroom) Termitimyces clypeatus, in sterilized medium containing 0.05 to 5.0% of 2-deoxy-D-glucose in addition to 0.5 to 2.0 % of cellobiose, succinate-0.5%, 2 to 3% of ammonium di hydrogen phosphate and conventional micro-nutrients at pH between 3 to 8 and incubating at temperatures between 20-35° C. under shaking in aerobic conditions, and separating the culture medium by known methods, and using the culture filtrate directly as the source of the enzyme cellobiase and also for endo-glucanase and cellobiohydrolase for use in cellulose hydrolysis.

A novel endoglucanase PPCE derived from Penicillium pinophilum, a cellulase preparation containing the endoglucanase PPCE, and a method of treating a cellulose-containing fabric utilizing the endoglucanase PPCE or the cellulase preparation, are disclosed. The endoglucanase PPCE is highly active to a fabric, and has a low optimum temperature and a strongly acidic optimum pH.

This invention provides a method for enhancing the cellobiase activity of the strain Termitimyces clypeatus using 2-deoxy-D-glucose as glycosylation inhibitor, said process comprises inoculating and growing mycelial culture of (the edible mushroom) Termitimyces clypeatus, in sterilized medium containing 0.05 to 5.0% of 2-deoxy-D-glucose in addition to 0.5 to 2.0% of cellobiose, succinate—0.5%, 2 to 3% of ammonium di hydrogen phosphate and conventional micro-nutrients at pH between 3 to 8 and incubating at temperatures between 20-35° C. under shaking in aerobic conditions, and separating the culture medium by known methods, and using the culture filtrate directly as the source of the enzyme cellobiase and also for endo-glucanase and cellobiohydrolase for use in cellulose hydrolysis.

The present invention relates to: an expression cassette which has an increased protein secretion in yeast compared to wild-type and which comprises a polynucleotide for coding a translational fusion partner (TFP) and a polynucleotide for coding a protein selected from the group consisting of: TrXynII, TrBx1, TrEGL2, PaCEL1, PaCel2, TeCBH1, NfCBH1, HgCBH1, CtCBH1, ClCBH2, and CfCex1; an expression vector comprising the same; a transformant in which the expression vector is introduced into a host cell; and a complex strain comprising two or more of the transformants. In addition, the present invention relates to a method for producing hemicellulase, endoglucanase, and exoglucanase, or a method for producing bioethanol, comprising a step for culturing the transformants. Furthermore, the present invention relates to a cellulase cocktail comprising endoglucanase and exoglucanase produced by the method and beta-glucosidase, and a method for saccharifying biomass using the cocktail. Moreover, the prevent invention relates to a method for producing bioenergy or useful biochemicals from cellulosic biomass, using a single strain which produces the cellulase, or two or more strains in combination.

The invention discloses an endoglucanase and a coding gene of the endoglucanase and an application of the endoglucanase and the coding gene. The endoglucanase is one type of the protein with the following amino acid residue sequence: 1) SEQ ID No.: 2 in the sequence table; 2) the amino acid residue sequence of the SEQ ID No.: 2 in the sequence table forms a protein with the endoglucanase activity by the substitution and / or the deletion and / or the addition of one or a plurality of amino acid residues. The endoglucanase and a coding gene of the endoglucanase has the advantages that: the endoglucanase has very high carboxymethyl cellulose enzyme activity (42229U / g), and the enzyme has a wide PH value range and a wide temperature range. The endoglucanase and the coding gene of the endoglucanase can be widely used in the degradation of cellulose.

The invention discloses a method for improving the yield of enzymatic hydrolysis of plant fiber materials. The method comprises the steps of carrying out alkaline pretreatment of the plant fiber materials and preparing monosaccharide by virtue of enzymatic hydrolysis of cellulose and xylanase and is characterized in that when cellulase and xylanase for hydrolyzing the plant fiber materials subjected to alkaline pretreatment are added, 10-50U / g of beta-glucosidase of cellulose is additionally added. Compared with the prior art, the method for improving the yield of enzymatic hydrolysis of the plant fiber materials has the prominent advantages that by excessively adding beta-glucosidase in an enzymatic hydrolysis system of the plant fiber materials subjected to alkaline pretreatment, the concentration of cellobiose in a hydrolysate generated at the initial stage of enzyme hydrolysis is reduced, the inhibition effect of cellobiose generated at the initial stage of enzyme hydrolysis on endoglucanase and exoglucanase is alleviated and thus the enzyme reaction rate and the yield of enzymatic hydrolysis are improved and the production cost for producing biomass sugar from the plant fiber materials subjected to alkaline pretreatment is reduced.

Described herein is a modified EGII cellulase having the amino acid sequence shown in SEQ ID NO:1, compositions comprising the modified EGII and methods of use.

The invention discloses an endoglucanase and a coding gene of the endoglucanase and an application of the endoglucanase and the coding gene. The endoglucanase is one type of the protein with the following amino acid residue sequence: 1) SEQ ID No.: 2 in the sequence table; 2) the amino acid residue sequence of the SEQ ID No.: 2 in the sequence table forms a protein with the endoglucanase activityby the substitution and / or the deletion and / or the addition of one or a plurality of amino acid residues. The endoglucanase and a coding gene of the endoglucanase has the advantages that: the endoglucanase has very high carboxymethyl cellulose enzyme activity (42229U / g), and the enzyme has a wide PH value range and a wide temperature range. The endoglucanase and the coding gene of the endoglucanase can be widely used in the degradation of cellulose.

Cleaning compositions comprising endo-β-1,3-glucanase enzyme and cleaning adjunct. Methods of treating surfaces including fabrics by contacting the surface with an aqueous was liquor having the cleaning composition therein. The compositions and methods are particularly for cleaning cotton fabrics. The compositions and methods are particularly for removal of soils containing callose, curdlan, pachyman, scleroglucan or schizophyllan. The compositions and methods are particularly for improving whiteness of a fabric, improved soil removal from a fabric, for malodour removal from a fabric, for anti-wrinkle benefits and / or for improved drying of a fabric.

Cleaning compositions and methods of cleaning comprising endo-beta-1,3-glucanase enzyme and surfactant. The weight ratio of surfactant to active endo-beta-1,3-glucanase enzyme protein is at least 1000:1. The compositions and methods are particularly for cleaning cotton fabrics.

The invention discloses an alkaliphilic streptomyces, a neutral endoglucanase produced by it and an application thereof. The alkaliphilic streptomyces is a neutral endoglucanase-producing strain screened from Shenzhen mangrove soil. The invention also discloses the neutral endoglucanase produced by the above-mentioned alkaliphilic streptomyces. The enzyme has a wide range of pH adaptability, and is suitable for washing and papermaking industries such as heat resistance, alkali, metal ions and surfactants. Enzymatic properties, can be used in industries such as washing and papermaking. The alkaliphilic streptomyces of the present invention belongs to alkali-resistant extremophile microorganisms, can be cultivated under high alkaline conditions, has the characteristics of effectively inhibiting contamination by miscellaneous bacteria, and is beneficial to large-scale fermentation production.

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.