40 results about "Endoglucanase Y" patented technology

The present invention relates to a detergent composition comprising an endoglucanase that provides improved detergency performance. The invention also relates to a detergent composition comprising a combination of an endoglucanase and other enzymes. One aspect of the invention relates to a process of washing a fabric or hard surface with the endoglucanase containing detergent.

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.

The invention discloses basophilic streptomyce, neutral endoglucanase produced by basophilic streptomyce and application of basophilic streptomyce. Basophilic streptomyce is a strain which is screened from soil of mangrove in Shenzhen and is capable of producing the neutral endoglucanase. The neutral endoglucanase has excellent enzymatic characteristics, is applicable to industries of washing and papermaking, is wide in pH application range and capable of resisting heat, alkali, metal ions and surfactants and can be applied to industries of washing and papermaking. Basophilic streptomyce belongs to an alkaline-resisting extreme microorganism, can be cultured at high-alkaline conditions and is capable of effectively restraining microbial contamination and beneficial to large-scale fermentation production.

The invention discloses a trichoderma reesei engineering bacterium high in secretion of endoglucanase and beta-glucosidases. The trichoderma reesei engineering bacterium is named as trichoderma reesei(Trichoderma reesei) QMEB5, the genotype of the bacterial strain is QM9414 delta Eegl2 delta Ebgl1, trichoderma reesei QM9414 is used as an original strain, the expression vector pTeg2 of a fusion gene of a gene expression box containing endoglucanase 2 is transformed, then the plasmid pTHB of a gene expression box containing beta-glucosidases is transformed, and screening and verifying are performed, so that the trichoderma reesei engineering bacterium is obtained. The invention further discloses an application of the engineering strain to fermentation production of cellulases. The experiment confirms that the egl2 expression quantity of the engineering strain is 27 times higher than that of the original strain, the bgl1 expression quantity of the engineering strain is 410 times higher than that of the original strain, the activity of the endoglucanase is 117.5IU / mL, and the activity of the beta-glucosidases is 34.5IU / mL. The cellulase system produced through fermentation can be efficiently applied to degradation of corn cobs and other lignocellulolyticenzyme materials, and has favorable industrial development and application prospects.

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.

A novel endoglucanase PPCE derived from Penicillium pinophilum, a cellulase preparation containing the endoglucanase PPCE, and a method of treating a cellulose-containing fabric utilizing the endoglucanase PPCE or the cellulase preparation, are disclosed. The endoglucanase PPCE is highly active to a fabric, and has a low optimum temperature and a strongly acidic optimum pH.

This invention provides a method for enhancing the cellobiase activity of the strain Termitimyces clypeatus using 2-deoxy-D-glucose as glycosylation inhibitor, said process comprises inoculating and growing mycelial culture of (the edible mushroom) Termitimyces clypeatus, in sterilized medium containing 0.05 to 5.0% of 2-deoxy-D-glucose in addition to 0.5 to 2.0% of cellobiose, succinate—0.5%, 2 to 3% of ammonium di hydrogen phosphate and conventional micro-nutrients at pH between 3 to 8 and incubating at temperatures between 20-35° C. under shaking in aerobic conditions, and separating the culture medium by known methods, and using the culture filtrate directly as the source of the enzyme cellobiase and also for endo-glucanase and cellobiohydrolase for use in cellulose hydrolysis.

Described herein is a modified EGII cellulase having the amino acid sequence shown in SEQ ID NO:1, compositions comprising the modified EGII and methods of use.

The invention discloses an endoglucanase and a coding gene of the endoglucanase and an application of the endoglucanase and the coding gene. The endoglucanase is one type of the protein with the following amino acid residue sequence: 1) SEQ ID No.: 2 in the sequence table; 2) the amino acid residue sequence of the SEQ ID No.: 2 in the sequence table forms a protein with the endoglucanase activityby the substitution and / or the deletion and / or the addition of one or a plurality of amino acid residues. The endoglucanase and a coding gene of the endoglucanase has the advantages that: the endoglucanase has very high carboxymethyl cellulose enzyme activity (42229U / g), and the enzyme has a wide PH value range and a wide temperature range. The endoglucanase and the coding gene of the endoglucanase can be widely used in the degradation of cellulose.

Cleaning compositions and methods of cleaning comprising endo-beta-1,3-glucanase enzyme and surfactant. The weight ratio of surfactant to active endo-beta-1,3-glucanase enzyme protein is at least 1000:1. The compositions and methods are particularly for cleaning cotton fabrics.

An object is to identify endoglucanase and β-glucosidase genes by isolating genomic DNA containing cellulase genes, which are classified into endoglucanases or β-glucosidases, from Acremonium cellulolyticus, and sequencing the nucleotide sequences thereof. The inventors intensively compared the amino acid sequences of known endoglucanases and β-glucosidases with each other to find conserved region of amino acid sequences in Acremonium cellulolyticus, and various primers were designed based on the information. PCR was carried out using the various primers thus designed and genomic DNA or cDNA as a template. As a result, gene fragments of endoglucanases and β-glucosidases were obtained. Primers were designed based on the gene fragments, and PCR was carried out to amplify nine genes of endoglucanases and β-glucosidases. The nucleotide sequences thereof were sequenced, and the present invention was completed.

The invention discloses an alkaliphilic streptomyces, a neutral endoglucanase produced by it and an application thereof. The alkaliphilic streptomyces is a neutral endoglucanase-producing strain screened from Shenzhen mangrove soil. The invention also discloses the neutral endoglucanase produced by the above-mentioned alkaliphilic streptomyces. The enzyme has a wide range of pH adaptability, and is suitable for washing and papermaking industries such as heat resistance, alkali, metal ions and surfactants. Enzymatic properties, can be used in industries such as washing and papermaking. The alkaliphilic streptomyces of the present invention belongs to alkali-resistant extremophile microorganisms, can be cultivated under high alkaline conditions, has the characteristics of effectively inhibiting contamination by miscellaneous bacteria, and is beneficial to large-scale fermentation production.

The present invention relates to novel cellulase enzymes, especially novel endoglucanases including endoglucanase fusion proteins, preparations and compositions containing these endoglucanase enzymes and fusion proteins, expression vectors, host cells and methods for their preparation and uses of the cellulases, preparations and compositions in the textile, detergent and pulp and paper industries.