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Method for improving yield of enzymatic hydrolysis of plant fiber materials

A plant fiber and enzymatic hydrolysis technology, applied in the direction of fermentation, etc., can solve the problems of low cellulase hydrolysis reaction speed and hydrolysis yield, etc., and achieve the effect of improving the enzymatic reaction rate and enzymatic hydrolysis yield, and reducing production costs.

Inactive Publication Date: 2015-01-14
NANJING FORESTRY UNIV
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  • Description
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AI Technical Summary

Problems solved by technology

[0004] Cellulase belongs to the hydrolytic enzymes of feedback inhibition. In the process of hydrolyzing cellulose into glucose by cellulase, the production of the final product glucose has an inhibitory effect on the activity of β-glucosidase, and the activity of β-glucosidase is inhibited. Finally, it leads to the accumulation of cellobiose in the hydrolysis process, and the accumulation of cellobiose inhibits endoglucanase and exoglucanase, resulting in low cellulase hydrolysis reaction speed and hydrolysis yield

Method used

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  • Method for improving yield of enzymatic hydrolysis of plant fiber materials
  • Method for improving yield of enzymatic hydrolysis of plant fiber materials
  • Method for improving yield of enzymatic hydrolysis of plant fiber materials

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: the pretreatment of corn stalk

[0026] Crush corn stalks to 2-5cm, use 8% (w / w) alkali, 40% sulfide, and 1:6 (w / v) solid-liquid ratio. After idling at 60°C for 0.5h, raise the temperature To 140°C, keep warm for 1h. The corn stalks pretreated by green liquor are washed with water to remove residual chemicals, refined and squeezed dry, and used as substrates for enzymatic hydrolysis. Analyze the moisture, cellulose, and xylan content of the substrate, and calculate the recovery of cellulose and xylan.

[0027] The results show that the moisture content of corn stalks after green liquor pretreatment, refining, washing, and squeezing is 76.90%, the cellulose content is 55.39% (dry basis), and the xylan content is 25.43% (dry basis). The total recovery rate was 63.31%, the recovery rate of cellulose was 91.95%, and the recovery rate of xylan was 63.42%.

Embodiment 2

[0028] Embodiment 2: Auxiliary addition of a small amount and the influence of excess β-glucosidase on enzymatic hydrolysis yield

[0029]Take by weighing the green liquid pretreatment corn stalk 10.82g (absolute dry weight is 2.50g) of embodiment 1 respectively in 12 250mL conical flasks (enzymolysis substrate concentration 5% (w / v)), in each conical flask Add 2.5mL of 1mol / L citric acid buffer solution to cellulose, add cellulase according to the enzyme dosage of 25FPU / g cellulose, add xylanase according to the enzyme dosage of 120U / g cellulose, respectively, according to 0, 1, 2 , 3, 4, 5, 10, 15, 20, 25, 37.5, 50U / g of cellulose were added to the amount of β-glucosidase, and an appropriate amount of distilled water was added in each Erlenmeyer flask so that the total volume of water in the enzymolysis system was 50 mL, mix the reaction system well with a glass rod, cover it, and enzymolyze it in a constant temperature shaker at 150 rpm and 50°C for 48 hours. After the hyd...

Embodiment 3

[0033] Example 3: Effect of Excessive Auxiliary Addition of β-Glucosidase on Enzymatic Hydrolysis of Corn Stove Pretreated with Different Substrate Concentrations

[0034] Take by weighing respectively the green liquor pretreatment corn stalk 5.41,10.82,16.23,21.65,27.06,32.47,43.28g (absolute dry weight is respectively 1.25,2.50,3.75,5.00,6.25,7.50,10.00g) in 7 In each 250mL Erlenmeyer flask (corresponding to 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, 15%, 20% (w / v) of enzymolysis substrate concentration), add 1mol / L citrate buffer 2.5mL, add cellulase according to the enzyme dosage of 25FPU / g cellulose respectively, add xylanase according to the enzyme dosage of 120U / g cellulose respectively, add β according to the dosage of 20U / g cellulose respectively -Glucosidase, add an appropriate amount of distilled water to each Erlenmeyer flask to make the total volume of water in the enzymolysis system 50mL, mix the reaction system thoroughly with a glass rod, cover the lid, and keep the temp...

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Abstract

The invention discloses a method for improving the yield of enzymatic hydrolysis of plant fiber materials. The method comprises the steps of carrying out alkaline pretreatment of the plant fiber materials and preparing monosaccharide by virtue of enzymatic hydrolysis of cellulose and xylanase and is characterized in that when cellulase and xylanase for hydrolyzing the plant fiber materials subjected to alkaline pretreatment are added, 10-50U / g of beta-glucosidase of cellulose is additionally added. Compared with the prior art, the method for improving the yield of enzymatic hydrolysis of the plant fiber materials has the prominent advantages that by excessively adding beta-glucosidase in an enzymatic hydrolysis system of the plant fiber materials subjected to alkaline pretreatment, the concentration of cellobiose in a hydrolysate generated at the initial stage of enzyme hydrolysis is reduced, the inhibition effect of cellobiose generated at the initial stage of enzyme hydrolysis on endoglucanase and exoglucanase is alleviated and thus the enzyme reaction rate and the yield of enzymatic hydrolysis are improved and the production cost for producing biomass sugar from the plant fiber materials subjected to alkaline pretreatment is reduced.

Description

technical field [0001] The invention relates to the technical field of enzymatic hydrolysis of plant fiber raw materials, in particular to a method for improving the yield of enzymatic hydrolysis of plant fiber raw materials. Background technique [0002] With the increasing demand and over-exploitation of fossil resources represented by human society, petroleum resources will inevitably face the fate of depletion; at the same time, the greenhouse effect caused by the use of a large number of fossil resources may evolve into a catastrophe for human beings. Ecological disaster. How will human beings deal with the future trends of resource and energy shortages, environmental pollution and global warming? It has become an international consensus to take the road of sustainable development. The production of bioenergy, biobased chemicals and biobased materials from renewable plant fiber resources is an important part of sustainable development strategies. my country has abund...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
Inventor 勇强储秋露余世袁李鑫徐勇欧阳嘉
Owner NANJING FORESTRY UNIV
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